FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487861

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487861
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences365440
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22200.6074868651488616No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAATTTCAT11600.31742556917688264No Hit
GTCCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGTTTACAGGA11460.31359457092819615No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC9900.2709063047285464No Hit
CACTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAATTTCAT9480.25941330998248685No Hit
GGATAATAGGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTAACATT8550.23396453590192645No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGT8040.22000875656742555No Hit
GTCCTACAATTCGTTGACATACTTTCCAATCAATAGGCCTGTTTACAGGA7680.21015761821366027No Hit
ATATGTTCCTGTGGTAAGGAGCCCCAACTCCCAATTACATATCCCATGAA7450.2038638353765324No Hit
GTTTACAGGAAGTTTCCTAAAACATGATTTGATTTTTTGTACAATATGTT7370.20167469352014009No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7070.193465411558669No Hit
TCCAAAGAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA7060.19319176882661998No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTACTGAAAGTCCAA6920.18936077057793346No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA6780.18552977232924694No Hit
GTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAATTAATTTTAGTC6660.1822460595446585No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC6630.18142513134851138No Hit
GTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGG6390.17485770577933452No Hit
TTGTAGGACTTTTGGGGTTCGCTGCCCCTTTTACACAATGTGGATATCCT6260.17130035026269702No Hit
ATCTAAGGGCAAGTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAA6100.16692206654991243No Hit
ACTCACAACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT6010.1644592819614711No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTTTGTATGCATGTATA5940.16254378283712784No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG5900.1614492119089317No Hit
CTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT5900.1614492119089317No Hit
ATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGTCTGGA5710.15625No Hit
GTATACATGCATACAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAA5580.15269264448336253No Hit
CCTACTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5560.15214535901926443No Hit
GTTGATAGGTGTAAGCAATTTGTGGGACCCCTAACAATAAATGAAAACAG5530.15132443082311733No Hit
CCTTCGTTTACGTCCCGTCGGCGCTGAATCCTGCGGACGACCCCTCCCGG5340.14612521891418565No Hit
GGAACATATTGTACAAAAAATCAAATCATGTTTTAGGAAACTTCCTGTAA5330.1458515761821366No Hit
CCTTACCACAGGAACATATTGTACAAAAAATCAAATCATGTTTTAGGAAA5250.14366243432574433No Hit
ACCAAATACTTGCCCTTAGATAAAGGAATCAAACCCTATTATCCAGAGCA5090.1392841506129597No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5060.1384632224168126No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA4930.13490586690017514No Hit
ATGTAATTGGGAGTTGGGGCTCCTTACCACAGGAACATATTGTACAAAAA4870.13326401050788092No Hit
GGTGTAAGCAATTTGTGGGACCCCTAACAATAAATGAAAACAGGAGACTA4840.13244308231173382No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGGCCCCCATG4750.12998029772329248No Hit
CCCTTAGATAAAGGAATCAAACCCTATTATCCAGAGCATGTAGTTAATCA4730.1294330122591944No Hit
GTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTG4710.12888572679509633No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA4700.12861208406304728No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT4640.12697022767075308No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA4620.12642294220665498No Hit
GTGGTAAGGAGCCCCAACTCCCAATTACATATCCCATGAAATTAAGGGAA4600.1258756567425569No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC4520.12368651488616463No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACATGATTTGATTTTTTGT4520.12368651488616463No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA4470.12231830122591944No Hit
TCCTCGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCGGTGCTGGTGGTTG4420.12095008756567426No Hit
ATGTGGATATCCTGCTTTAATGCCTTTGTATGCATGTATACAAGCTAAAC4410.12067644483362522No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG4370.11958187390542908No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4350.119034588441331No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG4160.1138353765323993No Hit
CAATAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGATTTTAT4130.1130144483362522No Hit
ATCCCGGGAATTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTAAAAA4130.1130144483362522No Hit
TCTATATGCAGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATT4050.1108253064798599No Hit
CAGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAACA4020.11000437828371278No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGT3970.10863616462346762No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG3970.10863616462346762No Hit
GGTCTATGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCTTGGC3960.10836252189141855No Hit
GCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGA3950.10808887915936952No Hit
CTTTAATCCTGATTGGCAAACTCCTTCTTTTCCTGACATTCATTTGAAGG3940.1078152364273205No Hit
GCTCTGGATAATAGGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTA3930.10754159369527146No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGATTA3900.10672066549912433No Hit
GTACAATATGTTCCTGTGGTAAGGAGCCCCAACTCCCAATTACATATCCC3870.10589973730297723No Hit
TTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA3850.10535245183887916No Hit
GTCTTACATAAGAGGACTCTTGGACTTTCAGTAATGTCAACGACCGACCT3780.10343695271453592No Hit
GGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA3760.10288966725043783No Hit
GTGTTGTCTCTCTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAA3760.10288966725043783No Hit
CCTTTATCTAAGGGCAAGTATTTGGTAACATTGGGATAAAATCTAGCAGG3750.10261602451838879No Hit
GTAATTGGGAGTTGGGGCTCCTTACCACAGGAACATATTGTACAAAAAAT3700.10124781085814359No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC3690.10097416812609457No Hit
GCTTTATTCTTCTACGGTACCTAACTTTAATCCTGATTGGCAAACTCCTT3670.1004268826619965No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)