FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002487862

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487862
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences658973
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCGTGTTCAGG40420.613378696851009No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30030.4557091109954429No Hit
CTTATATAGAATGCCAGCTTTCCACAGAGTATGTAAATAATGCCTAGTTT27530.41777128956725085No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCGTAT24070.3652653447106331No Hit
ATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAGTTAATTATG21370.3242924975681856No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAACTTTAATCTCCTTTTTTCA19730.2994052867112917No Hit
CTATTAATAATGTCCTCTTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG17240.2616192165688124No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAGTTAATTATGCCTGCTAGG16410.2490238598546526No Hit
GTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCC15930.24173979814043975No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT15600.2367320057119184No Hit
CTCTTATATAGAATGCCAGCTTTCCACAGAGTATGTAAATAATGCCTAGT15290.23202771585482257No Hit
CAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAGTTAAT15230.23111720814054598No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT15140.22975144656913107No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA15060.22853743628342893No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG14560.22094987199779048No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGT13830.20987202814075845No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA13520.2051677382836626No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC13270.2013739561408434No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA13010.19742842271231142No Hit
GGATAATACGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT12550.1904478635695241No Hit
GTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTTAGGATAGAAC12380.18786809171240704No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA12310.18680583271241766No Hit
CAATTTGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAGTTAAT12200.18513656856957722No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT12080.183315553141024No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTATTCCCTTAATTTCAT11990.18194979156960908No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC11960.18149453771247076No Hit
TTTCAGTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACAT11900.18058402999819417No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCGTGTTCA11820.17937001971249203No Hit
CACATATTGTTGACATCTATTAATAATGTCCTCTTGTAAATGAATGTGAG11580.17572798885538557No Hit
CAATCCTTGAGTTTCAGTACAATATGTTCCTGCGGTAAAGTACCCCAACT11390.172844714426843No Hit
CACAAATTGTTGACATCTATTAATAATGTCCTCTTGTAAATGAATGTGAG11030.16738166814118333No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAAG10160.1541793062841725No Hit
GGGACTTAGTAGTCAGCCATGTCAATGTTAATATGGGCCTAAAAATCAGA10140.15387580371274695No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA9920.15053727542706605No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACACAGTCTTTGA9890.15008202156992775No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC9840.1493232651413639No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC9460.1435567162842787No Hit
GTTTTGAAGTAATGATTAACTGCGTGTTCAGGATAATACGGTTTAATGCC9340.14173570085572548No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT9340.14173570085572548No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC9330.14158394957001275No Hit
CTCATAAGGTGGGAAACTTTACTGGTCTTTATTCTTCTACTGTACCTGTC9260.14052169057002334No Hit
GGATAGAACCTAGCAGGCATAATTAACTTTAATCTCCTTTTTTCATTAAC9260.14052169057002334No Hit
GTATTATCCTGAACACGCAGTTAATCATTACTTCAAAACTAGGCATTATT9220.13991468542717228No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG9200.13961118285574675No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA9200.13961118285574675No Hit
CAAGCATTGTTTTCGAAAACTGCCTGTAAATCGACCTATTGATTGGAAAG9200.13961118285574675No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGACCAGTAAAGTTTCCCAC9080.13779016742719352No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9050.13733491357005523No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCGCCATGC9000.13657615714149138No Hit
GCAGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAAC8910.13521039557007647No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8890.13490689299865094No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC8670.13156836471297004No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTGGAGGTTCCTGG8600.13050610571298066No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA8540.12959559799870404No Hit
GGCCCACATATTGTTGACATCTATTAATAATGTCCTCTTGTAAATGAATG8400.12747107999872528No Hit
GCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA8390.12731932871301252No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA8270.12549831328445932No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG8100.12291854142734225No Hit
TTGACATACTTTCCAATCAATAGGTCGATTTACAGGCAGTTTTCGAAAAC8090.12276679014162947No Hit
ATGATTAACTGCGTGTTCAGGATAATACGGTTTAATGCCTTTGTCCAAGG8000.12140102857021456No Hit
AAGCAGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGA7980.12109752599878901No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC7820.11866950542738473No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA7660.11624148485598046No Hit
CTTATATAGAATGCCCGCTTTCCACAGAGTATGTAAATAATGCCTAGTTT7660.11624148485598046No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT7640.1159379822845549No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG7640.1159379822845549No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA7570.11487572328456554No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7510.11396521557028892No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA7490.11366171299886339No Hit
ATTTACATACTCTGTGGAAAGCTGGCATTCTATATAAGAGAGAAACTACA7420.11259945399887401No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA7420.11259945399887401No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGTCTTTATTC7400.11229595142744847No Hit
GTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTATTCCCTTAAT7320.11108194114174633No Hit
CAGCCATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGT7310.11093018985603356No Hit
ATCTAAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGT7250.11001968214175695No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA7180.10895742314176757No Hit
AAAACTCAGACAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAA7140.1083504179989165No Hit
GCATTAAACCGTATTATCCTGAACACGCAGTTAATCATTACTTCAAAACT7060.10713640771321435No Hit
ATATTGTACTGAAACTCAAGCATTGTTTTCGAAAACTGCCTGTAAATCGA7060.10713640771321435No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGTCTTTATTCTTCTACTG7040.10683290514178882No Hit
GCTCCGAACGCAGGGTCCAACTGGTGATCGGGAAAGAATCCCAGAGGATT7000.10622589999893775No Hit
ATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAGTTAATTATG6990.10607414871322497No Hit
GATTAACTGCGTGTTCAGGATAATACGGTTTAATGCCTTTGTCCAAGGGC6910.10486013842752283No Hit
GTTCAGGATAATACGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTA6910.10486013842752283No Hit
CATGTATACAAGCTAAGCGGGCTTTCACTTTCTCGCCAACTTACAAGGCC6860.10410138199895898No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6850.10394963071324623No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA6810.10334262557039516No Hit
TACATATCCCATGAAATTAAGGGAATAGCCCCAACGTTTGGTTTTATTAG6790.1030391229989696No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT6770.10273562042754407No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG6620.10045935114185253No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)