FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002487863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences658973
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCGTGTTCAGG40380.612771691708158No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN29910.4538880955668897No Hit
CTTATATAGAATGCCAGCTTTCCACAGAGTATGTAAATAATGCCTAGTTT23290.35342874442503713No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCGTAT21790.3306660515681219No Hit
ATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAGTTAATTATG20480.31078663313974925No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAACTTTAATCTCCTTTTTTCA19630.297887773854164No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT18660.2831678991400255No Hit
CTATTAATAATGTCCTCTTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG17230.2614674652830996No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAGTTAATTATGCCTGCTAGG16660.2528176419974718No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA16080.24401606742613127No Hit
GTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCC15220.23096545685483322No Hit
CTCTTATATAGAATGCCAGCTTTCCACAGAGTATGTAAATAATGCCTAGT15180.23035845171198213No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG14950.22686817214058846No Hit
CAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAGTTAAT14100.21396931285500315No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC13350.20258796642654556No Hit
GTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTTAGGATAGAAC13310.20198096128369447No Hit
CAATTTGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAGTTAAT13080.1984906817123008No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT12510.189840858426673No Hit
GGATAATACGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT12220.18544007114100275No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAAG12100.18361905571244952No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGT12090.18346730442673675No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA11830.17952177099820477No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA11750.17830776071250262No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC11630.17648674528394942No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCGTGTTCA11400.17299646571255575No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTATTCCCTTAATTTCAT10910.16556065271263012No Hit
CAATCCTTGAGTTTCAGTACAATATGTTCCTGCGGTAAAGTACCCCAACT10540.1599458551412577No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC10520.15964235256983214No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC10260.15569681914130018No Hit
GTATTATCCTGAACACGCAGTTAATCATTACTTCAAAACTAGGCATTATT10130.15372405242703419No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA10060.1526617934270448No Hit
TTTCAGTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACAT9970.1512960318556299No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA9900.15023377285564052No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT9840.1493232651413639No Hit
CTCATAAGGTGGGAAACTTTACTGGTCTTTATTCTTCTACTGTACCTGTC9670.14674349328424685No Hit
CACATATTGTTGACATCTATTAATAATGTCCTCTTGTAAATGAATGTGAG9580.14537773171283194No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9560.14507422914140639No Hit
GTTTTGAAGTAATGATTAACTGCGTGTTCAGGATAATACGGTTTAATGCC9450.14340496499856595No Hit
CACAAATTGTTGACATCTATTAATAATGTCCTCTTGTAAATGAATGTGAG9390.14249445728428933No Hit
GCAGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAAC9300.14112869571287442No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC9250.1403699392843106No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA9160.13900417771289567No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG9130.13854892385575737No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC9030.13703141099862967No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGACCAGTAAAGTTTCCCAC9000.13657615714149138No Hit
GGATAGAACCTAGCAGGCATAATTAACTTTAATCTCCTTTTTTCATTAAC8800.13354113142723603No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG8710.1321753698558211No Hit
GGCCCACATATTGTTGACATCTATTAATAATGTCCTCTTGTAAATGAATG8600.13050610571298066No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT8490.1288368415701402No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGTCTTTATTCTTCTACTG8390.12731932871301252No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG8360.12686407485587423No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA8300.1259535671415976No Hit
GGGACTTAGTAGTCAGCCATGTCAATGTTAATATGGGCCTAAAAATCAGA8240.125043059427321No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8090.12276679014162947No Hit
ATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAGTTAATTATG8050.1221597849987784No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA7980.12109752599878901No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA7820.11866950542738473No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7770.11791074899882088No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA7670.11639323614169321No Hit
CAAGCATTGTTTTCGAAAACTGCCTGTAAATCGACCTATTGATTGGAAAG7650.11608973357026767No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA7540.11442046942742722No Hit
ATTTTGTGGTCTGTAAGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA7530.11426871814171446No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA7460.11320645914172509No Hit
AAGCAGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGA7420.11259945399887401No Hit
GCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA7310.11093018985603356No Hit
ATTTACATACTCTGTGGAAAGCTGGCATTCTATATAAGAGAGAAACTACA7190.10910917442748035No Hit
GCTCCGAACGCAGGGTCCAACTGGTGATCGGGAAAGAATCCCAGAGGATT7180.10895742314176757No Hit
ATATTGTACTGAAACTCAAGCATTGTTTTCGAAAACTGCCTGTAAATCGA7180.10895742314176757No Hit
GATTAACTGCGTGTTCAGGATAATACGGTTTAATGCCTTTGTCCAAGGGC7100.10774341285606542No Hit
GTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTATTCCCTTAAT7090.10759166157035265No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGTCTTTATTC7050.10698465642750157No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC7030.10668115385607604No Hit
ATGATTAACTGCGTGTTCAGGATAATACGGTTTAATGCCTTTGTCCAAGG7020.10652940257036328No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCGAT7010.1063776512846505No Hit
TTGACATACTTTCCAATCAATAGGTCGATTTACAGGCAGTTTTCGAAAAC6960.10561889485608666No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTGGAGGTTCCTGG6920.10501188971323559No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACACAGTCTTTGA6890.1045566358560973No Hit
GTCCAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATAA6870.10425313328467176No Hit
GCATTAAACCGTATTATCCTGAACACGCAGTTAATCATTACTTCAAAACT6860.10410138199895898No Hit
GTTCAGGATAATACGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTA6820.1034943768561079No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT6760.1025838691418313No Hit
CTTATATAGAATGCCCGCTTTCCACAGAGTATGTAAATAATGCCTAGTTT6750.10243211785611853No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA6710.10182511271326745No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA6630.10061110242756532No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT6590.10000409728471425No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)