FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002487864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences724027
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN83801.1574154002544104No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT26620.36766584671566116No Hit
CTTTTATATAAGATACCCGCCTTCCACAGAGTGTGTAAATAATGTCTCGT24250.33493226081347793No Hit
CCCTTACAGTAAATGAAAACAGAAGACTAAAATTAATTATGCCTGCTAGG20940.28921573366739084No Hit
TCCACAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA17340.2394938310311632No Hit
GTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGG15990.22084811754257785No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA15920.21988130276909562No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT14250.19681586460173445No Hit
CGTTAATATGGGCCTAAAAATTAGACAACTATTGTGGTTTCACATTTCCT13610.18797641524418288No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA12820.17706521994345514No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC12550.17333607724573807No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA12290.16974505094423273No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC11130.1537235489836705No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT10480.14474598322990717No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT9980.13784016341931998No Hit
ATATGTTCCTGTGGCAGTGTGCCCCAACTCCCAATTACATATCCCATGAA9570.13217739117463853No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG9430.1302437616276741No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT9420.13010564523146237No Hit
TTATTGATAGATGTAATCAATTTGTGGGGCCCCTTACAGTAAATGAAAAC9090.12554780415647485No Hit
CTTTATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGC8810.12168054506254602No Hit
ATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAACCTTAT8770.12112807947769905No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG8740.12071373028906379No Hit
ATGATTAACTACATGCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGG8710.12029938110042857No Hit
GGACATTATTGATAGATGTAATCAATTTGTGGGGCCCCTTACAGTAAATG8660.11960879911936985No Hit
CAGTAAATGAAAACAGAAGACTAAAATTAATTATGCCTGCTAGGTTTTAT8480.11712270398755847No Hit
GATGTAATCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGAAGACTA8340.11518907444059406No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC8310.11477472525195884No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA8260.1140841432709001No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA8200.11325544489362965No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC8020.11076934976181826No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT7850.10842137102621864No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT7850.10842137102621864No Hit
ATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGCATAA7830.10814513823379514No Hit
CTCTTTTATATAAGATACCCGCCTTCCACAGAGTGTGTAAATAATGTCTC7810.10786890544137166No Hit
ACTACATGCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATA7810.10786890544137166No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG7700.10634962508304249No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA7450.1028967151777489No Hit
ACATTATTGATAGATGTAATCAATTTGTGGGGCCCCTTACAGTAAATGAA7450.1028967151777489No Hit
GCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA7400.10220613319669018No Hit
CTTCTACACCTGTTGTACCGACCGACCACGGGGCGCACCTCTCTTTACGC7350.10151555121563145No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA7340.10137743481941973No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG7330.10123931842320798No Hit
ATATAAGATACCCGCCTTCCACAGAGTGTGTAAATAATGTCTCGTCTGGA7300.10082496923457275No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)