FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002487865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences724027
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN83371.1514763952173055No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT28130.3885214225436344No Hit
CTTTTATATAAGATACCCGCCTTCCACAGAGTGTGTAAATAATGTCTCGT23700.32733585902183204No Hit
CCCTTACAGTAAATGAAAACAGAAGACTAAAATTAATTATGCCTGCTAGG18160.2508193755205262No Hit
TCCACAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA17480.24142746057812758No Hit
GTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGG16690.23051626527739988No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT15690.2167046256562255No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA15300.21131808620396755No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC13520.1867333676782772No Hit
CGTTAATATGGGCCTAAAAATTAGACAACTATTGTGGTTTCACATTTCCT12530.17305984445331457No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA10720.14806077673898901No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA10660.14723207836171856No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT10010.1382545126079552No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT9980.13784016341931998No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT9950.13742581423068476No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC9880.13645899945720258No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG9780.13507783549508512No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT9540.13176304198600328No Hit
ATATGTTCCTGTGGCAGTGTGCCCCAACTCCCAATTACATATCCCATGAA9100.12568592055268657No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG8820.12181866145875775No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA8810.12168054506254602No Hit
CTTCTACACCTGTTGTACCGACCGACCACGGGGCGCACCTCTCTTTACGC8570.11836575155346417No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA8520.11767516957240545No Hit
CTTTATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGC8510.1175370531761937No Hit
ATGATTAACTACATGCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGG8380.11574154002544103No Hit
ATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAACCTTAT8290.11449849245953536No Hit
ACTACATGCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATA8170.11284109570499443No Hit
GCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA8080.11159804813908873No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC8040.11104558255424177No Hit
ATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGCATAA7980.11021688417697129No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC7930.10952630219591258No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCGTCTGACTTCTTTCCTTCTATTCG7870.10869760381864212No Hit
CTCTTTTATATAAGATACCCGCCTTCCACAGAGTGTGTAAATAATGTCTC7800.10773078904515992No Hit
ATATAAGATACCCGCCTTCCACAGAGTGTGTAAATAATGTCTCGTCTGGA7760.10717832346031295No Hit
GGACATTATTGATAGATGTAATCAATTTGTGGGGCCCCTTACAGTAAATG7480.10331106436638413No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG7430.1026204823853254No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA7360.1016536676118432No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT7300.10082496923457275No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)