FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487867

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487867
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences480032
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGTGTGTTCAGG14300.29789680687954134No Hit
ATGATTAACTGTGTGTTCAGGATAATAAGGTTTAATTCCTTTATCTAAGG13050.2718568762082528No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG12670.26394073728418105No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG12360.2574828344777015No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA11440.23831744550363307No Hit
CCCGCGTGTCCTGGCCAAAATTTGCAGTCCCCAACCTCCAATCACTCACC11390.23727584827678153No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT11360.2366508899406706No Hit
ATTTACAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT10970.22852643157122857No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10110.21061095926938203No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTATAAA10070.2097776814879008No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT9410.19602859809346043No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9380.1954036397573495No Hit
ATACCTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA9170.19102893140457303No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA8640.17998800079994667No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATG8400.17498833411105927No Hit
GTATAAAGGGATTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC8190.17061362575828276No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGA8120.1691553896406906No Hit
CCTTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT7980.16623891740550628No Hit
ATCAACAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAG7840.16332244517032196No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC7130.14853176454903005No Hit
GGATAATAAGGTTTAATTCCTTTATCTAAGGGCAAATATTTGGTACGGTT7130.14853176454903005No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG7110.14811512565828944No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7110.14811512565828944No Hit
ATGAGTAACTGTGTGTTCAGGATAATAAGGTTTAATTCCTTTATCTAAGG7080.1474901673221785No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC7020.14624025064995666No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG6900.14374041730551296No Hit
AGTCTGTACAACATCTTGAATCCCTTTATACCGCTGTTACCAATTTTCTT6900.14374041730551296No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG6790.14144890340643956No Hit
GTTTAATTCCTTTATCTAAGGGCAAATATTTGGTACGGTTAGGAAAGAAC6780.14124058396106925No Hit
CATTTAAACCCTGCTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT6760.14082394507032864No Hit
ATACTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA6610.137699153389774No Hit
GTCTGTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6570.13686587560829278No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAA6380.1329078061462569No Hit
CTCCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG6320.13165788947403506No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA6320.13165788947403506No Hit
ACCACGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC6210.12936637557496167No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTATACC5960.12415838944070397No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATAGCTGAGAGTCCAA5960.12415838944070397No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5840.12165855609626024No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC5840.12165855609626024No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5740.11957536164255715No Hit
GATTAACTGTGTGTTCAGGATAATAAGGTTTAATTCCTTTATCTAAGGGC5660.11790880607959468No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5510.11478401439904005No Hit
GCCTAGTCATCTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGC5500.11457569495366975No Hit
GCCTAATCATCTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGC5490.11436737550829944No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA5440.1133257782814479No Hit
TACCGTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5380.11207586160922604No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA5350.11145090327311512No Hit
CCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATG5340.11124258382774481No Hit
CAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGA5260.10957602826478234No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC5260.10957602826478234No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5190.10811779214719018No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5130.10686787547496833No Hit
GGAAAGTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGT5040.10499300046663555No Hit
ATCTAAGGGCAAATATTTGGTACGGTTAGGAAAGAACCTAGCAGGCATAA5020.10457636157589494No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC4990.10395140323978401No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC4960.10332644490367308No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC4940.10290980601293247No Hit
ATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAA4940.10290980601293247No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG4920.10249316712219185No Hit
TTAGCAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGC4890.10186820878608092No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACC4880.10165988934071062No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)