FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487868

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487868
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences527198
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18310.3473078425942436No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG13840.2625199640362824No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA12700.24089620977317822No Hit
GTGCTGGTGAACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC12590.23880970716884362No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT12390.23501606607005338No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT11940.22648037359777543No Hit
GCACCGGACCATGCAAAACCTGCACAACGCCTGCTCAAGGAACCTCTATG10950.20770185015876388No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10240.19423442425805865No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC10150.19252728576360306No Hit
GGATAATATGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT9540.1809566804122929No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT9140.1733693982147125No Hit
ATCTTAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG9090.17242098794001495No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA9050.1716622597202569No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT8910.16900671095110376No Hit
AATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATTGGGA8750.1659717980720716No Hit
GACACAAAGGTTCCACGCATGCGCTGATGGCCTATGGCCAAGCCCCAACC8650.1640749775226765No Hit
GAAAACAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTATCCCAATGT8480.1608503825887048No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA8350.15838451587449118No Hit
TGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTATCCCAATG8210.15572896710533804No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC8140.15440119272076147No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA8040.15250437217136634No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT7960.15098691573185027No Hit
GCATCTAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTAC7770.14738295668799958No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT7730.14662422846824155No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA7680.145675818193544No Hit
GAAAAAAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTATCCCAATGT7320.1388472642157216No Hit
ATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAA6960.1320187102378992No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT6740.12784570502923No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG6710.12727665886441147No Hit
CTCCAAACGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA6540.1240520639304398No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA6470.12272428954586323No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT6450.12234492543598419No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG6350.12044810488658911No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC6320.11987905872177056No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG6270.11893064844707302No Hit
CATTTAAATCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT6260.1187409663921335No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG6210.11779255611743596No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC6150.1166544637877989No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTATTCG6140.11646478173285939No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG6140.11646478173285939No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG6130.11627509967791987No Hit
CTTTAGGGCATGGACATTGACACGTATAAAGAATTTGGAGCTTCTGTGGA6070.1151370073482828No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6030.11437827912852477No Hit
CTATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGT5910.11210209446925064No Hit
GACATAAAGGTTCCACGCATGCGCTGATGGCCTATGGCCAAGCCCCAACC5850.11096400213961358No Hit
ATCCCCTGGGATTCTTCCCCGATCATCAGTTGGACCCTGCATTCAAAGCC5830.11058463802973456No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA5830.11058463802973456No Hit
GGATATCCACATTGCGTGAAAGGGGCAGCAAACCCCAAAAGACCCACAAT5750.10906718159021847No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG5700.10811877131552093No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5640.10698067898588387No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG5580.10584258665624681No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG5490.1041354481617912No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC5470.10375608405191218No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCAGCA5380.1020489455574566No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC5370.10185926350251708No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)