FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002487869

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487869
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences527198
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18160.34446261177015086No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG14570.2763667540468666No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT12960.24582794320160548No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA12180.23103274291632367No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT11770.22325577866380372No Hit
GTGCTGGTGAACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC11590.21984150167489255No Hit
GGATAATATGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT10710.20314948084021564No Hit
ATCTTAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG10080.1911995113790265No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC9880.18740587028023625No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9620.182474136851809No Hit
GCACCGGACCATGCAAAACCTGCACAACGCCTGCTCAAGGAACCTCTATG9420.17868049575301878No Hit
GAAAACAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTATCCCAATGT8790.16673052629182963No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA8720.16540275190725306No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA8290.1572464235448541No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT8180.1551599209405195No Hit
GACACAAAGGTTCCACGCATGCGCTGATGGCCTATGGCCAAGCCCCAACC7940.15060755162197126No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT7930.15041786956703174No Hit
TGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTATCCCAATG7810.14814168490775761No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA7790.1477623207978786No Hit
AATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATTGGGA7760.14719327463306006No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC7680.145675818193544No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT7610.1443480438089674No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT7320.1388472642157216No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA7270.13789885394102405No Hit
GAAAAAAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTATCCCAATGT7230.13714012572126602No Hit
GCATCTAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTAC7200.13657107955644748No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG6980.13239807434777826No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG6670.12651793064465344No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG6530.12386238187550029No Hit
ATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAA6520.12367269982056078No Hit
CATTTAAATCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT6450.12234492543598419No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA6360.1206377869415286No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG6340.1202584228316496No Hit
CTCCAAACGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA6340.1202584228316496No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG6240.11836160228225448No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA6220.11798223817237546No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC6180.11722350995261742No Hit
CTTTATGTCTCCTCTGCCGATCCATACTGCGGAACTCCTAGCCGCTTGTT6160.1168441458427384No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6060.1149473252933433No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT5980.11342986885382722No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC5970.11324018679888771No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG5940.11267114063406916No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC5760.10925686364515799No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5750.10906718159021847No Hit
CTTTAGGGCATGGACATTGACACGTATAAAGAATTTGGAGCTTCTGTGGA5670.1075497251507024No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC5660.10736004309576289No Hit
CTATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGT5650.10717036104082338No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG5620.10660131487600483No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC5550.10527354049142826No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTATTCG5430.10299735583215414No Hit
CCTTCAAATGAATGTCAGGAAAAGAAGGAGTTTGCCATTTAGGATTAAAG5370.10185926350251708No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG5300.1005314891179405No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA5300.1005314891179405No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5290.100341807063001No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)