FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487870

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487870
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences520844
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN27820.5341330609549116No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA26710.5128214974157329No Hit
GTATACATGCATATAAAGGCATCAAAGCAGGATATCCACATTGTGTAAAA23420.4496547910698789No Hit
CATGTATACAATCCAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGTCC19550.37535231278463416No Hit
ATATATAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTACAAA16420.31525754352550855No Hit
ATTACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATT15900.3052737479936411No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT14170.27205842824338955No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAGTAAT13370.25669874280974725No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGCGAGAGGACAACAGAGT13270.25477878213054195No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT13020.24997888043252875No Hit
CTCCAAAGCTGACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTT11360.21810753315772094No Hit
CCTCCCGCTCACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10900.2092757140333766No Hit
CGGTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGT10700.20543579267496603No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC10370.19909992243358857No Hit
TATCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT10370.19909992243358857No Hit
CATATAAAGGCATCAAAGCAGGATATCCACATTGTGTAAAAGGGGCAGCA10340.19852393422982698No Hit
CTTTTACACAATGTGGATATCCTGCTTTGATGCCTTTATATGCATGTATA10030.19257205612429057No Hit
ATTCTACGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA9880.18969211510548264No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG9810.18834814263003893No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGACTTGTAAGTTGGCGA9710.18642818195083363No Hit
GGAACATATTGTACAAAAAATCAAGCAATGTTTTCGAAAATTGCCTGTAA9620.1847002173395489No Hit
GGATATCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT9380.1800923117094562No Hit
CTATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGACGGAAATTGCACC9360.17970831957361513No Hit
CCCTTAACTTCATGGGATATATAATTGGAAGTTGGGGTACTTTACCACAG9310.17874833923401248No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAA9180.17625239035104562No Hit
CAAGTAATGATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAA8920.17126049258511186No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC8820.16934053190590656No Hit
ATGTGGATATCCTGCTTTGATGCCTTTATATGCATGTATACAATCCAAGC8590.1649246223437344No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG8330.15993272457780064No Hit
GTCTTACATAAGCGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT8220.15782076783067484No Hit
CTAGTAAACTGAGCCAAGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT7950.15263687399682055No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA7920.15206088579305896No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG7830.15033292118177422No Hit
GGTCTATTTACAGGCAATTTTCGAAAACATTGCTTGATTTTTTGTACAAT7720.14822096443464836No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT7620.1463010037554431No Hit
ATACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA7610.14610900768752255No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA7590.1457250155516815No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA7530.1445730391441583No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC7450.1430370706007941No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCGCTTATGT7340.1409251138536683No Hit
GCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGCAGAGACCTGACC7330.14073311778574774No Hit
GTCCAAGAGTCCGCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC7310.1403491256499067No Hit
GTGGATATCCTGCTTTGATGCCTTTATATGCATGTATACAATCCAAGCAG7300.14015712958198615No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTATATATCCCATGAA7140.1370851924952577No Hit
GGCCAACCACGGGGCGTACCTCTCTTTACGCGGTCTCCCCGTCTGTGCCT7120.13670120035941663No Hit
CTACCGTCCCCTTCTTCATCTGCCGTTCCGGCCGACCACGGGGCGTACCT7070.13574122001981398No Hit
GTCGTTGACATTGCTGAGAGTCCAAGAGTCCGCTTATGTAAGACCTTGGG7030.1349732357481319No Hit
AAGGTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACACAGTCTTT7000.1343972475443703No Hit
ATTATATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATT6980.13401325540852924No Hit
GGTTCAGATATTGTTTACACAGAAAGGACTTGTAAGTTGGCGAGAAAGTG6900.132477286865165No Hit
CCTAGTACAAGTAATGATTAACCTAATCTCCTCCCCCAACTCCTCCCAGT6850.13151730652556237No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG6680.12825337337091336No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA6640.12748538909923127No Hit
GTCTTTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6490.12460544808042331No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTAC6480.1244134520125028No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA6460.12402945987666172No Hit
GTGTTGCTCCAGACCGGCTGCGAGCAAAACAAGCTGCTAGGAGTTCCGCA6410.12306947953705909No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC6310.12114951885785379No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA6310.12114951885785379No Hit
TTGACATACTTTCCAATCAACAGGTCTATTTACAGGCAATTTTCGAAAAC6300.12095752278993327No Hit
ATATAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTACAAAAA6290.12076552672201273No Hit
AAATAGACCTGTTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGG6270.12038153458617167No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6270.12038153458617167No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA6220.11942155424656901No Hit
ACCTTGAGGCATACTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTG6190.11884556604280744No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG6150.11807758177112533No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGACGGAAATTGC6130.11769358963528427No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT6110.11730959749944321No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG6070.1165416132277611No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCAGGGTCCCGTG6030.11577362895607898No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG5980.11481364861647633No Hit
GCACGGGACCCTGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG5960.11442965648063527No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5920.11366167220895317No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA5900.1132776800731121No Hit
GTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTGTGTTTAAAGACT5840.11212570366558894No Hit
CTTTATATGCATGTATACAATCCAAGCAGGCTTTCACTTTCTCGCCAACT5810.11154971546182735No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC5790.11116572332598629No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG5650.10847777837509887No Hit
CCTCTGCACGTCGCATGGAAACCACCGTGAACGCCCACCAGGTCTTGCCC5630.10809378623925783No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5600.10751779803549624No Hit
ATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCC5590.1073258019675757No Hit
GCTTTGGAGTGTGGATTCGCACTCCTCCCGCTCACAGACCACCAAATGCC5550.10655781769589359No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA5530.10617382556005253No Hit
ACGAACCACTGAACAAATGGCACTAGTAAACTGAGCCAAGAGAAACGGGC5530.10617382556005253No Hit
ATATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTACAAA5500.10559783735629096No Hit
GGATATATAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTACA5460.10482985308460882No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAGTAATGAT5400.10367787667708565No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5390.10348588060916511No Hit
CTTTACCACAGGAACATATTGTACAAAAAATCAAGCAATGTTTTCGAAAA5250.10079793565827772No Hit
GTCTATTTACAGGCAATTTTCGAAAACATTGCTTGATTTTTTGTACAATA5250.10079793565827772No Hit
GCCGAACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAAACCAC5230.10041394352243665No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG5220.10022194745451614No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)