FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002487871

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487871
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences520844
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN27550.5289491671210573No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA24300.4665504450468854No Hit
GTATACATGCATATAAAGGCATCAAAGCAGGATATCCACATTGTGTAAAA22960.44082297194553455No Hit
CATGTATACAATCCAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGTCC16940.32524133905737607No Hit
ATATATAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTACAAA16820.32293738624232976No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT13940.2676425186812174No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT13440.25804271528519096No Hit
ATTACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATT13160.2526668253834161No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAGTAAT12760.244986982666595No Hit
CCTCCCGCTCACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11790.22636336407830365No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGCGAGAGGACAACAGAGT10950.21023569437297923No Hit
CTCCAAAGCTGACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTT10940.2100436983050587No Hit
CTTTTACACAATGTGGATATCCTGCTTTGATGCCTTTATATGCATGTATA10210.1960279853468601No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGACTTGTAAGTTGGCGA9620.1847002173395489No Hit
CTATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGACGGAAATTGCACC9440.18124428811697937No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC9130.17529241001144297No Hit
TATCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT9060.17394843753599926No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG9000.17279646112847608No Hit
CCCTTAACTTCATGGGATATATAATTGGAAGTTGGGGTACTTTACCACAG8920.17126049258511186No Hit
CATATAAAGGCATCAAAGCAGGATATCCACATTGTGTAAAAGGGGCAGCA8850.16991652010966815No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAA8640.16588460268333705No Hit
GGAACATATTGTACAAAAAATCAAGCAATGTTTTCGAAAATTGCCTGTAA8500.16319665773244965No Hit
CTACCGTCCCCTTCTTCATCTGCCGTTCCGGCCGACCACGGGGCGTACCT8470.16262066952868803No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG8450.162236677392847No Hit
GTCTTACATAAGCGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT8360.16050871278156223No Hit
GGATATCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT8310.15954873244195958No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC8240.1582047599665159No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA8140.1562847992873106No Hit
GGTCTATTTACAGGCAATTTTCGAAAACATTGCTTGATTTTTTGTACAAT8130.15609280321939006No Hit
ATTCTACGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA8070.15494082681186688No Hit
CGGTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGT7950.15263687399682055No Hit
GGCCAACCACGGGGCGTACCTCTCTTTACGCGGTCTCCCCGTCTGTGCCT7810.14994892904593313No Hit
ATGTGGATATCCTGCTTTGATGCCTTTATATGCATGTATACAATCCAAGC7530.1445730391441583No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTATATATCCCATGAA7470.14342106273663516No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA7320.1405411217178272No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG7120.13670120035941663No Hit
ATATAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTACAAAAA7010.13458924361229083No Hit
GGTTCAGATATTGTTTACACAGAAAGGACTTGTAAGTTGGCGAGAAAGTG7010.13458924361229083No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA6960.13362926327268818No Hit
GCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGCAGAGACCTGACC6960.13362926327268818No Hit
ATACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA6700.12863736550675442No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6630.1272933930313107No Hit
CTAGTAAACTGAGCCAAGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT6620.12710139696339018No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC6620.12710139696339018No Hit
GTGGATATCCTGCTTTGATGCCTTTATATGCATGTATACAATCCAAGCAG6590.12652540875962862No Hit
AAATAGACCTGTTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGG6570.12614141662378756No Hit
GTCCAAGAGTCCGCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC6560.12594942055586703No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA6450.1238374638087412No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG6430.12345347167290015No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG6400.12287748346913854No Hit
GTCTTTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6390.12268548740121803No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT6290.12076552672201273No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6280.12057353065409221No Hit
CAAGTAATGATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAA6230.11961355031448956No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6190.11884556604280744No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCGCTTATGT6110.11730959749944321No Hit
ATTATATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATT6040.11596562502399951No Hit
GTCGTTGACATTGCTGAGAGTCCAAGAGTCCGCTTATGTAAGACCTTGGG5920.11366167220895317No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA5920.11366167220895317No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA5880.11289368793727103No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5860.11250969580143No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGACGGAAATTGC5860.11250969580143No Hit
GCACGGGACCCTGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG5840.11212570366558894No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTAC5750.11039773905430417No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA5740.11020574298638364No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5710.10962975478262206No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5530.10617382556005253No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC5520.10598182949213199No Hit
CTTTACCACAGGAACATATTGTACAAAAAATCAAGCAATGTTTTCGAAAA5510.10578983342421147No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA5470.10502184915252935No Hit
CTTTATATGCATGTATACAATCCAAGCAGGCTTTCACTTTCTCGCCAACT5440.10444586094876776No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5390.10348588060916511No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA5370.10310188847332408No Hit
GTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTGTGTTTAAAGACT5360.10290989240540353No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG5350.10271789633748302No Hit
ATATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTACAAA5350.10271789633748302No Hit
GCTTTGGAGTGTGGATTCGCACTCCTCCCGCTCACAGACCACCAAATGCC5320.10214190813372143No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCAGGGTCCCGTG5270.10118192779411878No Hit
ATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCC5260.10098993172619825No Hit
TTGACATACTTTCCAATCAACAGGTCTATTTACAGGCAATTTTCGAAAAC5240.1006059395903572No Hit
CTCTTGGACTCTCAGCAATGTCAACGACCGACCTTGAGGCATACTTCAAA5220.10022194745451614No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)