FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435191
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA17970.4129221422318016No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG17930.41200300557686165No Hit
CTTATATAGAATGCCAGCTTTCCACAGAGTATGTAAATAATGCCTAGTTT16580.38098214347263615No Hit
ATCCTAACCTTACCAAATATTTGCCTTTGGACAAAGGCATTAAACCGTAT14740.3387018573453955No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13680.3143447359894851No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG12150.2791877589380295No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10750.24701797601512898No Hit
TTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCT10700.24586905519645397No Hit
CATTTAAACCCTAACAAAACTAAGAGATGGGGTTATTCTCTACATTTCAT10680.24540948686898398No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCGTTGACAGACT10340.23759682530199383No Hit
ATCACACATCATACAAAAAATCAAAGATTGTTTTAGAAAACTCCCTGTTA9970.2290948112437987No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA9680.2224310704954836No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA9300.21369927227355345No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9170.21071207814499843No Hit
CTCTTATATAGAATGCCAGCTTTCCACAGAGTATGTAAATAATGCCTAGT8980.20634617903403335No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT8840.20312920074174326No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC8680.19945265412198324No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8660.1989930857945132No Hit
GCCTAGCAGCCATGGAAATGATGTATATTTGCGGGCGAGAACAACAGAGT8350.1918697767187281No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACAC8220.18888258259017304No Hit
ATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTAT7970.18313797849679797No Hit
TATCCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCG7850.18038056853197793No Hit
CTCCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG7840.18015078436824292No Hit
GGATAATACGGTTTAATGCCTTTGTCCAAAGGCAAATATTTGGTAAGGTT7800.1792316477133029No Hit
GTTTACACAGAAAGGCCTTGTAAGTCGGTGAGAATGTGAAAGCCCGTTTG7520.17279769112872279No Hit
CAATAGGCCTGTTAACAGGGAGTTTTCTAAAACAATCTTTGATTTTTTGT7430.17072963365510777No Hit
GAATAACCCCATCTCTTAGTTTTGTTAGGGTTTAAATGTATACCCAAAGA7350.16889136034522773No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA7160.16452546123426265No Hit
CTAGTAAACTGAGCCAAGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT7080.1626871879243826No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6980.1603893462870326No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCTTTGGACAAAGGCATTAAAC6960.1599297779595626No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT6840.15717236799474255No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC6710.1541851738661875No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG6510.1495894905914874No Hit
GTTCAGGATAATACGGTTTAATGCCTTTGTCCAAAGGCAAATATTTGGTA6350.14591294397172735No Hit
GTAGAGAATAACCCCATCTCTTAGTTTTGTTAGGGTTTAAATGTATACCC6200.1424661815157023No Hit
ATGTAACACCTTGGGCAACACCAGGTGGGCGTTCACGGTGGTTTCCATGC6140.1410874765332923No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6120.14062790820582227No Hit
GACATAGCCCATGAAATGTAGAGAATAACCCCATCTCTTAGTTTTGTTAG5890.1353428724399172No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5890.1353428724399172No Hit
GGGTTATTCTCTACATTTCATGGGCTATGTCATTGGGGGTTATGGGTCCT5770.13258546247509714No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC5730.13166632582015714No Hit
CTTTTACACAATGTGGATATCCTGCCTTAATGCCCTTGTATGCATGTATT5710.13120675749268712No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5690.1307471891652171No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA5660.1300578366740121No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG5630.12936848418280708No Hit
ATGTGTGATTGTGGCAAGGACCCATAACCCCCAATGACATAGCCCATGAA5610.12890891585533706No Hit
GAATACATGCATACAAGGGCATTAAGGCAGGATATCCACATTGTGTAAAA5550.12753021087292707No Hit
GTTAACAGGGAGTTTTCTAAAACAATCTTTGATTTTTTGTATGATGTGTG5540.12730042670919206No Hit
ATTCTCTACATTTCATGGGCTATGTCATTGGGGGTTATGGGTCCTTGCCA5500.12638129005425203No Hit
GTCCAAGAGTCCTCTTATGTAACACCTTGGGCAACACCAGGTGGGCGTTC5360.12316431176196199No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCAGGGTCCCGTG5340.12270474343449199No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5310.12201539094328698No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATACGGTTTAATGCC5290.12155582261581697No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5270.12109625428834696No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGGAAAATTCCTATGGGAG5220.11994733346967193No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT5180.11902819681473194No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC5110.11741970766858689No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5040.11581121852244187No Hit
CAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGT4990.11466229770376686No Hit
GGATATCCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT4950.11374316104882684No Hit
GTTTAATGCCTTTGTCCAAAGGCAAATATTTGGTAAGGTTAGGATAGAAC4950.11374316104882684No Hit
GTCTTTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA4890.11236445606641682No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT4830.11098575108400678No Hit
CGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGGCAGACGAAGAAGG4820.11075596692027179No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA4790.11006661442906679No Hit
GGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGGCCGACCACGGGGCG4770.10960704610159677No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA4760.10937726193786176No Hit
GTAGAGTCCCAAGCGGCCCCGAGAAGGGTCGTCCGCGGGATTCAGCGCCG4740.10891769361039176No Hit
GTATATTTGCGGGCGAGAACAACAGAGTTGTCAGTCCCGAGAATGTTTGC4730.10868790944665677No Hit
ACCTGACCCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTG4710.10822834111918675No Hit
CTTTTGGAGTGTGGATTCGCACTCCTACCGCTTACAGACCACCAAATGCC4700.10799855695545174No Hit
CAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTT4650.10684963613677673No Hit
GAAATGTAGAGAATAACCCCATCTCTTAGTTTTGTTAGGGTTTAAATGTA4630.10639006780930671No Hit
GTGTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT4620.10616028364557173No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGAATTGTGGGTCTTTTGGGTTTT4580.10524114699063171No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGG4570.10501136282689669No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4550.10455179449942668No Hit
CTCCTCGACACCGCCTCTGCTCTGTATCGGGAGGCCTTAGAGTCTCCGGA4500.10340287368075167No Hit
ATTTACATACTCTGTGGAAAGCTGGCATTCTATATAAGAGAGAAACTACA4470.10271352118954666No Hit
GATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACAC4460.10248373702581165No Hit
GATTAACTGCATGTTCAGGATAATACGGTTTAATGCCTTTGTCCAAAGGC4450.10225395286207666No Hit
GTGGATATCCTGCCTTAATGCCCTTGTATGCATGTATTCAATCCAAACGG4440.10202416869834165No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4430.10179438453460665No Hit
CAAATATACATCATTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCC4420.10156460037087163No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC4410.10133481620713664No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)