FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487873

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487873
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435191
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG21370.49104875790170294No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA18240.41912631465264677No Hit
CTTATATAGAATGCCAGCTTTCCACAGAGTATGTAAATAATGCCTAGTTT14710.3380125048541905No Hit
ATCCTAACCTTACCAAATATTTGCCTTTGGACAAAGGCATTAAACCGTAT12530.28791955715995965No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG12200.2803366797567045No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11760.27022617655236436No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCGTTGACAGACT11530.26494114078645925No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10780.24770732850633398No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA10250.23552876782837878No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA9790.22495869629656864No Hit
CTCTTATATAGAATGCCAGCTTTCCACAGAGTATGTAAATAATGCCTAGT9560.21967366053066353No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA9230.21209078312740842No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT9130.2097929414900584No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC8950.2056568265428283No Hit
ATCACACATCATACAAAAAATCAAAGATTGTTTTAGAAAACTCCCTGTTA8860.2035887690692133No Hit
TTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCT8700.19991222244945323No Hit
CATTTAAACCCTAACAAAACTAAGAGATGGGGTTATTCTCTACATTTCAT8700.19991222244945323No Hit
GCCTAGCAGCCATGGAAATGATGTATATTTGCGGGCGAGAACAACAGAGT8660.1989930857945132No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8360.1920995608824631No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA8240.18934215091764306No Hit
CTCCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG7810.1794614318770379No Hit
GGATAATACGGTTTAATGCCTTTGTCCAAAGGCAAATATTTGGTAAGGTT7730.17762315856715785No Hit
ATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTAT7310.16797222369028772No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG6980.1603893462870326No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT6950.15969999379582758No Hit
TATCCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCG6900.15855107297715257No Hit
GAATAACCCCATCTCTTAGTTTTGTTAGGGTTTAAATGTATACCCAAAGA6900.15855107297715257No Hit
CAATAGGCCTGTTAACAGGGAGTTTTCTAAAACAATCTTTGATTTTTTGT6880.15809150464968255No Hit
GTTTACACAGAAAGGCCTTGTAAGTCGGTGAGAATGTGAAAGCCCGTTTG6860.15763193632221253No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCTTTGGACAAAGGCATTAAAC6800.1562532313398025No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6680.1534958213749825No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACAC6570.15096819557389743No Hit
GTTCAGGATAATACGGTTTAATGCCTTTGTCCAAAGGCAAATATTTGGTA6540.1502788430826924No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA6520.14981927475522244No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6480.1489001381002824No Hit
CTAGTAAACTGAGCCAAGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT6430.14775121728160737No Hit
GGGTTATTCTCTACATTTCATGGGCTATGTCATTGGGGGTTATGGGTCCT6410.14729164895413738No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG6180.1420066131882323No Hit
CTTTTACACAATGTGGATATCCTGCCTTAATGCCCTTGTATGCATGTATT5950.1367215774223272No Hit
ATGTGTGATTGTGGCAAGGACCCATAACCCCCAATGACATAGCCCATGAA5930.1362620090948572No Hit
GTAGAGAATAACCCCATCTCTTAGTTTTGTTAGGGTTTAAATGTATACCC5900.1355726566036522No Hit
GGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGGCCGACCACGGGGCG5740.13189610998389212No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5720.13143654165642213No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATACGGTTTAATGCC5660.1300578366740121No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA5630.12936848418280708No Hit
GAATACATGCATACAAGGGCATTAAGGCAGGATATCCACATTGTGTAAAA5520.12684085838172204No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA5380.123623880089432No Hit
GTCTTTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5380.123623880089432No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5380.123623880089432No Hit
GACATAGCCCATGAAATGTAGAGAATAACCCCATCTCTTAGTTTTGTTAG5300.12178560677955197No Hit
ATTCTCTACATTTCATGGGCTATGTCATTGGGGGTTATGGGTCCTTGCCA5240.12040690179714193No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA5220.11994733346967193No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC5220.11994733346967193No Hit
GTTTAATGCCTTTGTCCAAAGGCAAATATTTGGTAAGGTTAGGATAGAAC5180.11902819681473194No Hit
GTAGAGTCCCAAGCGGCCCCGAGAAGGGTCGTCCGCGGGATTCAGCGCCG5150.11833884432352691No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5100.1171899235048519No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC5050.11604100268617688No Hit
ACCTGACCCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTG4980.11443251354003184No Hit
GGATATCCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT4970.11420272937629684No Hit
GTTAACAGGGAGTTTTCTAAAACAATCTTTGATTTTTTGTATGATGTGTG4960.11397294521256184No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGGAAAATTCCTATGGGAG4940.11351337688509182No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT4870.1119048877389468No Hit
ATGTAACACCTTGGGCAACACCAGGTGGGCGTTCACGGTGGTTTCCATGC4800.11029639859280178No Hit
CAAATATACATCATTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCC4790.11006661442906679No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCAGGGTCCCGTG4720.10845812528292174No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGAATTGTGGGTCTTTTGGGTTTT4690.10776877279171675No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4690.10776877279171675No Hit
CTCCTCGACACCGCCTCTGCTCTGTATCGGGAGGCCTTAGAGTCTCCGGA4670.10730920446424673No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT4660.10707942030051172No Hit
GTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCTTCTCGGGGCCG4650.10684963613677673No Hit
GCTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGGCCGACCACG4570.10501136282689669No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC4560.10478157866316169No Hit
GAACAAATGGCACTAGTAAACTGAGCCAAGAGAAACGGGCTGAGGCCCAC4540.10432201033569169No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC4540.10432201033569169No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4510.10363265784448668No Hit
GTGTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT4470.10271352118954666No Hit
CAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGT4460.10248373702581165No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG4450.10225395286207666No Hit
CATGTATTCAATCCAAACGGGCTTTCACATTCTCACCGACTTACAAGGCC4430.10179438453460665No Hit
ATGTGGATATCCTGCCTTAATGCCCTTGTATGCATGTATTCAATCCAAAC4370.10041567955219662No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)