FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487874

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487874
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498220
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA40550.8138974750110393No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN39240.787603869776404No Hit
CTCTATGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA18750.3763397695797038No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT15940.31993898277869215No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT14450.2900325157560917No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG13630.27357392316647267No Hit
CTCTACGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA12870.25831961783950863No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT12450.24988960700092328No Hit
CACTAATATGGGCCTAAAGTTTAGGCAACTATTGTGGTTTCACATTTCTT12350.24788246156316487No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT11520.23122315442976998No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT11320.22720886355425315No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT11130.22339528722251215No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT10890.21857813817189195No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC10560.21195455822728915No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG10290.2065352655453414No Hit
ATCTTAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG9990.20051382923206618No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTAC9990.20051382923206618No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC9880.19830596925053187No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT9660.19389024928746337No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT9470.1900766729557224No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA9220.18505880936132632No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT9210.18485809481755047No Hit
GAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCCAAATGTTA8500.1706073622094657No Hit
GTTATGTTAACACTAATATGGGCCTAAAGTTTAGGCAACTATTGTGGTTT8370.16799807314037976No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8140.1633816386335354No Hit
CTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAAACCCTAACAAA7980.16017020593312192No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC7910.158765204126691No Hit
TTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC7810.1567580586889326No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT7660.15374734053229497No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT7420.14893019148167477No Hit
CTCACAGTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT7370.14792661876279556No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT7140.14331018425595118No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT7070.1419051824495203No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT6950.1394966079242102No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT6940.13929589338043435No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG6930.1390951788366585No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACTACACATAGCGCC6750.13548231704869335No Hit
GTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCC6670.13387660069848661No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATGGCAGAGAGTCCAA6610.13267231343583155No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6610.13267231343583155No Hit
AGATCACATCGTACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTA6600.13247159889205573No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA6510.13066516799807315No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG6430.1290594516478664No Hit
GATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTGG6410.12865802256031472No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC6330.127052306210108No Hit
CTGTAACCAATTTTCTTTTGTCTCTGGGTATACATTTAAACCCTAACAAA6310.1266508771225563No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG6260.12564730440367708No Hit
ACCCATAACATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCAT6110.12263658624703946No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATTTACACACTCTATGGA6000.1204287262655052No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG5970.11982658263417767No Hit
ATCCAATATCCAGGGACCTAGTAGTCAGTTATGTTAACACTAATATGGGC5960.11962586809040182No Hit
TTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAAC5920.11882300991529846No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTACGATGTGAT5910.11862229537152262No Hit
GGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAA5880.1180201517401951No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT5850.11741800810886756No Hit
TCCAATATCCAGGGACCTAGTAGTCAGTTATGTTAACACTAATATGGGCC5770.11581229175866084No Hit
GTGCTGGTGCGCACACCAATTTATGCCTACAGCCTCCTAATACAAACAAC5760.11561157721488499No Hit
GTTTTATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAAC5760.11561157721488499No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5700.11440728995222994No Hit
ATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCCATGAA5690.1142065754084541No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGATAATAAGGTTTAATAC5620.1128015736020232No Hit
ACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCA5510.11059371362048893No Hit
GACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAG5480.10999156998916142No Hit
GTGTAGTTTCTCTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAA5460.10959014090160973No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA5450.10938942635783389No Hit
CTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC5440.10918871181405804No Hit
GTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACAT5440.10918871181405804No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCGTACAGAAA5300.10637870820119626No Hit
GTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCC5300.10637870820119626No Hit
CTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTA5290.10617799365742042No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5260.1055758500260929No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCCATGTCAACGACCGACCT5240.10517442093854121No Hit
ATATTTGCCATTGGATAAGGGTATTAAACCTTATTATCCAGAACATCTAG5170.10376941913211031No Hit
GCTTTATTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCT5110.10256513186945526No Hit
ATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATAC5080.10196298823812774No Hit
CCAATATCCAGGGACCTAGTAGTCAGTTATGTTAACACTAATATGGGCCT5020.10075870097547268No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)