FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498220
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN39210.7870017261450766No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA38900.7807795752880254No Hit
CTCTATGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA19470.39079121673156436No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT14540.29183894665007426No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT14020.2814017903737305No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG13400.26895748865962826No Hit
CTCTACGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA13330.2675524868531974No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT13000.2609289069085946No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC12020.24125888161856207No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT11820.23724459074304524No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG11340.2276102926418048No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT11250.22580386174782227No Hit
CACTAATATGGGCCTAAAGTTTAGGCAACTATTGTGGTTTCACATTTCTT11110.22299385813496048No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT10570.212155272771065No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT10540.21155312913973745No Hit
ATCTTAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG9850.19770382561920435No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTAC9830.19730239653165269No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT9610.19288667656858416No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA9190.1844566657299988No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC9010.18084380394203364No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC8620.1730159367347758No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT8600.1726145076472241No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT8580.17221307855967244No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT8510.17080807675324156No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8240.1653887840712938No Hit
GAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCCAAATGTTA8130.16318092408975954No Hit
CTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAAACCCTAACAAA8060.16177592228332865No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG7870.15796234595158765No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT7440.14933162056922644No Hit
GTTATGTTAACACTAATATGGGCCTAAAGTTTAGGCAACTATTGTGGTTT7360.1477259042190197No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG7160.14371161334350285No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT7060.14170446790574445No Hit
CTCACAGTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT7020.1409016097306411No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACTACACATAGCGCC7000.1405001806430894No Hit
CTGTAACCAATTTTCTTTTGTCTCTGGGTATACATTTAAACCCTAACAAA6990.14029946609931354No Hit
TTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC6940.13929589338043435No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT6880.1380916061177793No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT6780.13608446068002086No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT6780.13608446068002086No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6720.13488017341736583No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6660.13367588615471077No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT6360.1276544498414355No Hit
ATCCAATATCCAGGGACCTAGTAGTCAGTTATGTTAACACTAATATGGGC6330.127052306210108No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCCATGTCAACGACCGACCT6280.12604873349122878No Hit
GATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTGG6250.12544658985990123No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA6220.12484444622857373No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATGGCAGAGAGTCCAA6160.12364015896591868No Hit
GTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCC6130.12303801533459113No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA6040.12123158444060857No Hit
CTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTA5940.11922443900285015No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG5900.11842158082774677No Hit
ATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCCATGAA5900.11842158082774677No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGATAATAAGGTTTAATAC5870.11781943719641925No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATTTACACACTCTATGGA5740.11521014812733331No Hit
GTGTAGTTTCTCTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAA5710.11460800449600579No Hit
AGATCACATCGTACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTA5690.1142065754084541No Hit
GTGCTGGTGCGCACACCAATTTATGCCTACAGCCTCCTAATACAAACAAC5600.11240014451447153No Hit
ACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTT5590.11219942997069568No Hit
GTTTTATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAAC5570.11179800088314398No Hit
TTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAAC5450.10938942635783389No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAA5440.10918871181405804No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG5420.10878728272650637No Hit
TCCAATATCCAGGGACCTAGTAGTCAGTTATGTTAACACTAATATGGGCC5400.10838585363895467No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTACGATGTGAT5380.10798442455140299No Hit
CTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC5360.10758299546385132No Hit
GTTATGGGTCATTGCCACAAGATCACATCGTACAGAAAATCAAAGAATGT5350.10738228092007547No Hit
ACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCA5260.1055758500260929No Hit
GGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAA5240.10517442093854121No Hit
GTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACAT5160.10356870458833446No Hit
ATATTTGCCATTGGATAAGGGTATTAAACCTTATTATCCAGAACATCTAG5160.10356870458833446No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG5050.1013608446068002No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)