FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487876

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487876
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences466720
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT18640.3993829276654096No Hit
GGATAATACGGTTTGATCCCTTTATCCAAGGGCAAATATTTAGTAACATT15310.3280339389784025No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCCAAGGGCAAATA14950.32032053479602335No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT12550.26889784024682895No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12470.26718375042852244No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCGGAAAGTCCAA11880.25454233801851217No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG11120.23825848474460062No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA11050.23675865615358244No Hit
CATGTATACAAGCCAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC10910.23375899897154612No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA10850.23247343160781625No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC10720.22968803565306825No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCCAAGGGCAAATATTTAGTA10520.225402811107302No Hit
GGAACATATTGTACAAAAGATCAAAATGTGTTTTAGGAAACTTCCTGTAA10510.2251885498800137No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10480.2245457661981488No Hit
ATTCTTTGACATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTCCT10410.22304593760713062No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT10070.21576105587932806No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT9910.21233287624271513No Hit
GGAAATAATGATTAACTACATACTCTGGATAATACGGTTTGATCCCTTTA9560.20483373328762425No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA9020.19326362701405553No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT8850.18962118615015428No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG8730.18705005142269454No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC8620.18469317792252316No Hit
CTATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGT8520.18255056564964006No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC8070.17290881042166611No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATCTTTTGTACAATATGTT8050.17248028796708947No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAATCCCCCATG7740.16583818992115187No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTGTTGTGGTTTCACATTTCCT7610.16305279396640382No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA7290.15619643469317793No Hit
ATATTGTACAAAAGATCAAAATGTGTTTTAGGAAACTTCCTGTAAACAGG7220.15469660610215977No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA7150.15319677751114158No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT7150.15319677751114158No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT7130.15276825505656497No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATCTTTTGT7060.15126842646554678No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA6970.149340075419952No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC6970.149340075419952No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG6890.14762598560164553No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACACATAAGGTG6770.14505485087418582No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA6740.1444120671923209No Hit
GCGTAAAAGGGGCGGCAAATCCTAAAAGACCCACAATTCGTTGACATACT6710.14376928351045595No Hit
CAGTCTCTCTCCCAAAAGTAAGACAGGAAATGTGAAACCACAACAGTTGT6640.1422694549194378No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGAGACTA6590.14119814878299622No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC6520.13969832019197806No Hit
GTACAATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCC6370.13648440178265342No Hit
CCTTTATCCAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGG6290.13477031196434694No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG6260.134127528282482No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA6220.13327048337332875No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGTACGACAGAAT6220.13327048337332875No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA6150.1317706547823106No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAG6080.1302708261912924No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC6030.12919952005485089No Hit
GCTTACATCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAA5970.12791395269112102No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAG5890.12619986287281454No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT5890.12619986287281454No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG5860.1255570791909496No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA5820.12470003428179638No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG5810.12448577305450806No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC5800.12427151182721975No Hit
GATGTATACTTGCGGGAGAGTACGACAGAATTGTCAGTCCCGATGAGTTT5670.12148611587247173No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT5580.11955776482687694No Hit
GCGTGAAAGGGGCGGCAAATCCTAAAAGACCCACAATTCGTTGACATACT5550.118914981145012No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5470.11720089132670553No Hit
CTTTCACGCAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA5460.11698663009941722No Hit
ATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAA5450.11677236887212891No Hit
CAGTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTG5430.11634384641755229No Hit
CACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTTGC5360.11484401782653413No Hit
GTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTGAG5360.11484401782653413No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG5340.1144154953719575No Hit
TACCTTGACAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5290.11334418923551594No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA5260.112701405553651No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAGGTTCCCGTGGGCGTTC5250.1124871443263627No Hit
ACCTTGCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCTGACATTCATT5160.11055879328076791No Hit
CTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCTGAC5160.11055879328076791No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTGGATAAAGGGATCAAAC5050.1082019197805965No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC5030.10777339732601987No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG4960.10627356873500171No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA4940.1058450462804251No Hit
CTACATACTCTGGATAATACGGTTTGATCCCTTTATCCAAGGGCAAATAT4930.10563078505313678No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAGTCTCCTGTTTTCATTTAC4920.10541652382584847No Hit
GGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGCATAATTAATTT4910.10520226259856016No Hit
ATCCCAATGTTACTAAATATTTGCCCTTGGATAAAGGGATCAAACCGTAT4900.10498800137127184No Hit
TGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGA4880.10455947891669524No Hit
CATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTTGCTT4870.10434521768940694No Hit
GAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGGAAGATCCAGC4830.10348817278025368No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA4820.10327391155296538No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG4730.10134556050737059No Hit
CAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTAT4680.10027425437092903No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)