FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences466720
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT18580.39809736030167986No Hit
GGATAATACGGTTTGATCCCTTTATCCAAGGGCAAATATTTAGTAACATT14140.3029653753856702No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCCAAGGGCAAATA13300.28496743229345217No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12410.26589818306479257No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCGGAAAGTCCAA12110.2594703462461433No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT11960.2562564278368186No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA11200.23997257456290708No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA11080.23740143983544737No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG11070.23718717860815908No Hit
GGAACATATTGTACAAAAGATCAAAATGTGTTTTAGGAAACTTCCTGTAA10280.22026054165238257No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG9840.21083304765169694No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9820.21040452519712033No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC9750.20890469660610217No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT9660.20697634556050737No Hit
ATTCTTTGACATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTCCT9610.20590503942406582No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT9500.20354816592389444No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC9450.20247685978745286No Hit
CATGTATACAAGCCAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC9350.20033424751456974No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT9210.19733459033253342No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC9150.19604902296880355No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCCAAGGGCAAATATTTAGTA9090.19476345560507372No Hit
GGAAATAATGATTAACTACATACTCTGGATAATACGGTTTGATCCCTTTA8740.18726431264998286No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA8550.18319334933150497No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATCTTTTGTACAATATGTT8130.17419437778539595No Hit
CTATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGT7950.17033767569420638No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA7930.16990915323962977No Hit
ATATTGTACAAAAGATCAAAATGTGTTTTAGGAAACTTCCTGTAAACAGG7620.16326705519369214No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAATCCCCCATG7380.1581247857387727No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA7270.15576791223860129No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTGTTGTGGTTTCACATTTCCT7230.15491086732944806No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG7160.1534110387384299No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA7140.15298251628385326No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC7020.15041138155639355No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACACATAAGGTG6960.14912581419266369No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGAGACTA6880.14741172437435723No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT6790.14548337332876243No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA6760.1448405896468975No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC6670.1429122386013027No Hit
CAGTCTCTCTCCCAAAAGTAAGACAGGAAATGTGAAACCACAACAGTTGT6560.14055536510113129No Hit
GCGTAAAAGGGGCGGCAAATCCTAAAAGACCCACAATTCGTTGACATACT6500.13926979773740145No Hit
GTACAATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCC6480.13884127528282483No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAG6480.13884127528282483No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA6360.1362701405553651No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG6310.13519883441892355No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC6310.13519883441892355No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC6240.1336990058279054No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT6180.13241343846417553No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA6110.13091360987315734No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT6110.13091360987315734No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGTACGACAGAAT6010.12877099760027425No Hit
GCTTACATCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAA5850.12534281796366128No Hit
GTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTGAG5830.12491429550908469No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATCTTTTGT5770.12362872814535482No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA5760.1234144669180665No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTGGATAAAGGGATCAAAC5700.12212889955433666No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG5590.11977202605416525No Hit
CAGTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTG5580.11955776482687694No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5530.11848645869043538No Hit
CCTTTATCCAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGG5400.11570106273568735No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG5380.11527254028111072No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAG5290.11334418923551594No Hit
GCGTGAAAGGGGCGGCAAATCCTAAAAGACCCACAATTCGTTGACATACT5210.11163009941720947No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT5180.11098731573534454No Hit
CTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCTGAC5170.11077305450805622No Hit
ATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAA5170.11077305450805622No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA5140.11013027082619128No Hit
CTTTCACGCAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA5110.10948748714432636No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA5070.10863044223517312No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAGTCTCCTGTTTTCATTTAC5020.10755913609873156No Hit
GTACAACAGGCGGAGCAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG4960.10627356873500171No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAGGTTCCCGTGGGCGTTC4930.10563078505313678No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC4890.10477374014398354No Hit
CACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTTGC4830.10348817278025368No Hit
GCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAG4820.10327391155296538No Hit
TACCTTGACAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4790.10263112787110044No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA4750.10177408296194722No Hit
CTACATACTCTGGATAATACGGTTTGATCCCTTTATCCAAGGGCAAATAT4740.1015598217346589No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCCAAACAG4740.1015598217346589No Hit
GGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGCATAATTAATTT4740.1015598217346589No Hit
GTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG4710.10091703805279396No Hit
ACCTTGCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCTGACATTCATT4670.10005999314364072No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)