FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487880

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487880
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences590800
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN38130.6453960731211916No Hit
CATTTAAACACTACTAAAACTAAACGTTGGGGCTACTCACTTAACTTCAT25000.42315504400812454No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT21620.36594448205822616No Hit
ATTTACAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT20390.3451252538930264No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGTGTGTTCAGG18100.3063642518618822No Hit
CCCTTTACACAATGTGGTTACCCAGCCTTAATGCCTTTGTATGCATGTAT15840.26811103588354773No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA15020.2542315504400813No Hit
GTTTAAATGTATACCCAAAGACAGAAGAAAATTGGTAACAGCGGTAAAAA14730.24932295192958703No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT14420.24407582938388625No Hit
GTGTAAAGGGAGCGGCAAAGCCTAGAAGCCCCACAATTCGTTGACACACT14000.23696682464454977No Hit
CCTATAAATAGACCTATTGATTGGAAAGTGTGTCAACGAATTGTGGGGCT13010.22020988490182802No Hit
CCCAGTACAAAGATCATTAATCTAGTCTCCTCCCCCAACTCCTCCCAGTCTGTAAA12620.21360866621530128No Hit
CTACTAAAACTAAACGTTGGGGCTACTCACTTAACTTCATGGGATATGTA12160.20582261340555177No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTCTGTC11980.2027758970886933No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA11760.1990521327014218No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG11700.1980365605958023No Hit
GTAGTACGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT11630.19685172647257954No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA11330.19177386594448206No Hit
TTTACAGACTGGGAGGAGTTGGGGGAGGAGACTAGATTAATGATCTTTGTACTGGG11080.18754231550440081No Hit
GTTTTAGTAGTGTTTAAATGTATACCCAAAGACAGAAGAAAATTGGTAAC10130.1714624238320921No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC10100.17095463777928233No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC9740.16486120514556535No Hit
ATGTAAGACCTTGGGCAAGACCTGTCGGGCGTTCACGGTGGTCTCCATGC9670.1636763710223426No Hit
AGACTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT9080.15368991198375084No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGA8940.15132024373730535No Hit
GTCTTTGGGTATACATTTAAACACTACTAAAACTAAACGTTGGGGCTACT8830.1494583615436696No Hit
GTTCTATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC8450.1430264048747461No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAAT8300.14048747461069738No Hit
ATATGTTCCTGTGGTAAGGTCCCCCAACTTCCAATTACATATCCCATGAA8030.13591740013540962No Hit
GTATACATGCATACAAAGGCATTAAGGCTGGGTAACCACATTGTGTAAAG8000.13540961408259986No Hit
ATGTGGTATTGGGGGCCAAGACTGTACAACATCTTGAATCCCTTTTTACC7940.13439404197698035No Hit
ATTTTGTGGTCTGTAAGCAGGGGGAGTGCGAATCCACACTCCAAAAGACA7930.13422477995937712No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7900.13371699390656736No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGGTT7890.1335477318889641No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATG7890.1335477318889641No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA7850.13287068381855113No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG7790.13185511171293163No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT7770.13151658767772512No Hit
GCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAAC7680.12999322951929587No Hit
CTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTCTG7660.12965470548408936No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAAAGGACAACAGAGT7600.12863913337846986No Hit
GGACATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG7520.12728503723764387No Hit
ATGATTAACTGTGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGG7460.1262694651320244No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT7450.12610020311442113No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGACTGTACAACATCTTG7330.12406905890318212No Hit
GCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTTCAAAACCTTCGGA7260.12288422477995938No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC7220.12220717670954637No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG7160.12119160460392688No Hit
TCCTCGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTG7080.11983750846310089No Hit
CTTTCCAATCAATAGGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTG7060.11949898442789439No Hit
CAATAGGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGT7020.11882193635748138No Hit
TTTAAACACTACTAAAACTAAACGTTGGGGCTACTCACTTAACTTCATGG6930.11729857819905212No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC6890.11662153012863914No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGTG6890.11662153012863914No Hit
GGCCAAGACTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATT6700.11340555179417738No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTTGGGGGAGGAGACTA6680.11306702775897089No Hit
GATTAACTGTGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC6660.11272850372376439No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGACTGTACAACATCTTGAA6660.11272850372376439No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA6630.11222071767095464No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTGTTCG6470.10951252538930263No Hit
TATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAA6440.10900473933649289No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC6430.10883547731888965No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT6320.1069735951252539No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT6160.1042654028436019No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATGTCCTCTTTC6120.1035883547731889No Hit
GCACTAAGGCAAGCCATTCTATGTTGGGTTGAGTTGATGAATCTGGCCAC6110.10341909275558565No Hit
AGTTAAGTGAGTAGCCCCAACGTTTAGTTTTAGTAGTGTTTAAATGTATA6090.10308056872037916No Hit
GCCTTAATGCCTTTGTATGCATGTATACAAGCTAAACAGGCTTTCACTTT6070.10274204468517265No Hit
CAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGA6060.1025727826675694No Hit
CACTTAACTTCATGGGATATGTAATTGGAAGTTGGGGGACCTTACCACAG6050.10240352064996615No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5970.10104942450914015No Hit
CCCAAAGACAGAAGAAAATTGGTAACAGCGGTAAAAAGGGATTCAAGATG5940.1005416384563304No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT5910.10003385240352064No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)