FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002487882

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487882
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences689549
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN58890.8540364789159292No Hit
CAATTACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTTTG33440.4849546587697176No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGT23430.339787310256414No Hit
CAATGACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTTTG20520.29758581333596307No Hit
GGATAATAGGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTAACATT19370.2809082458244447No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA18080.2622003657463067No Hit
TCCAAAGAGTATGTAAATAATGTCTCGTTTGGAAGTAATGATTAACTACA17210.2495834233680275No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA15920.23087554328988946No Hit
ATATGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC15040.2181135785854232No Hit
ATGTCATTGGGAGTTGGGGCTCCTTGCCACAGGAACATATTGTACAAAAA14320.20767197109995084No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA13860.2010009440953435No Hit
CTGTTTACGCAGAAAGGCCTTATAAGTTGGCGAGAAAGTAAAAGCCTGTT13690.1985355645501625No Hit
ATGTAATTGGGAGTTGGGGCTCCTTGCCACAGGAACATATTGTACAAAAA13480.19549009570023307No Hit
CTATTATCCAGAGCATGTAGTTAATCATTACTTCCAAACGAGACATTATT13060.18939915800037416No Hit
GTTGATAGGTGTAAGCAATTTGTGGGACCCCTAACAGTAAATGAAAACAG12900.18707880078138028No Hit
ATCCTAATGTTACCAAATACTTGCCCTTAGATAAAGGAATCAAACCCTAT12340.17895755051490178No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT12270.17794239423159194No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAAGGGGCAGCG12120.17576705933878523No Hit
GGAACATATTGTACAAAAAATCAAATTATGTTTTAGAAAACTTCCTGTAA11830.17156141187935883No Hit
ACCAAATACTTGCCCTTAGATAAAGGAATCAAACCCTATTATCCAGAGCA11690.1695310993127392No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAATTTCAT11550.16750078674611957No Hit
ATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGTTTGGA11510.1669206974413711No Hit
GTATGTAAATAATGTCTCGTTTGGAAGTAATGATTAACTACATGCTCTGG11440.16590554115806128No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA11220.16271504998194472No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTTTATATGCGTGTATA11170.16198993835100914No Hit
CCCTAACAGTAAATGAAAACAGGAGATTGAAATTAATTATGCCTGCTAGA11120.16126482672007356No Hit
ATCTAAGGGCAAGTATTTGGTAACATTAGGATAAAATCTAGCAGGCATAA10640.15430375506309196No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC10630.15415873273690484No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA10610.1538686880845306No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA10610.1538686880845306No Hit
ACTCACGACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT10310.14951801829891712No Hit
GGTGTAAGCAATTTGTGGGACCCCTAACAGTAAATGAAAACAGGAGATTG10140.14705263875373614No Hit
GTGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAATTCGTTGACATACT10000.1450223261871165No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG9780.14183183501099994No Hit
ATGTAAGACCTTGGGCAGGTTGCGGTGGGCGTTCACGGTGGTCTCCATGC9600.13922143313963184No Hit
AATACGGGCCTAAAGATCAGACAACTATTGTGGTTTCACATTTCCTGTCT9380.13603094196351528No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT9240.13400062939689567No Hit
TTCTTCTACTGTACCTAACTTTAATCCTGATTGGCAAACTCCTTCTTTTC9160.13284045078739873No Hit
CAATAGGCCTGTTTACAGGAAGTTTTCTAAAACATAATTTGATTTTTTGT9080.1316802721779018No Hit
GTATTTGGTAACATTAGGATAAAATCTAGCAGGCATAATTAATTTCAATC8940.12964995961128217No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA8640.12529928982566865No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC8400.12181875399717786No Hit
CCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT8390.12167373167099076No Hit
ATGTTAATATGGGCCTAAAGATCAGACAACTATTGTGGTTTCACATTTCC8380.12152870934480364No Hit
CATTAGGATAAAATCTAGCAGGCATAATTAATTTCAATCTCCTGTTTTCA8280.12007848608293246No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCGTGTATACAAGCTAAAC8250.11964341910437111No Hit
CAGTAAATGAAAACAGGAGATTGAAATTAATTATGCCTGCTAGATTTTAT8090.11732306188537726No Hit
GCTTACACCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAA7950.11529274931875763No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG7950.11529274931875763No Hit
CCCTTAGATAAAGGAATCAAACCCTATTATCCAGAGCATGTAGTTAATCA7810.11326243675213798No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT7740.11224728046882818No Hit
CTTTAATCCTGATTGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAGG7710.11181221349026683No Hit
GTTTACAGGAAGTTTTCTAAAACATAATTTGATTTTTTGTACAATATGTT7630.1106520348807699No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG7590.11007194557602143No Hit
GTATACACGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAA7580.10992692324983432No Hit
CCTTTATCTAAGGGCAAGTATTTGGTAACATTAGGATAAAATCTAGCAGG7500.10876674464033738No Hit
GCCTAGCAGCCATGGAAACGATGTATACTTGCGGGAGAGTACGACAGAAT7490.10862172231415027No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA7460.10818665533558892No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT7430.10775158835702756No Hit
CGTGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTATAAGGCC7370.10688145439990487No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA7320.10615634276896929No Hit
ATACCATATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA7210.104561097180911No Hit
GCTTTATTCTTCTACTGTACCTAACTTTAATCCTGATTGGCAAACTCCTT7180.10412603020234965No Hit
TTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA7170.10398100787616255No Hit
ATATGTTCCTGTGGCAAGGAGCCCCAACTCCCAATTACATATCCCATGAA7140.10354594089760118No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCGTGTATACAAGCTAAACAG7140.10354594089760118No Hit
GTCGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAAGCCCCCATG7120.10325589624522696No Hit
GTGTTGTCTCTCTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAA7010.10166065065716867No Hit
ACCCCTAACAGTAAATGAAAACAGGAGATTGAAATTAATTATGCCTGCTA6990.10137060600479443No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA6980.10122558367860733No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)