FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002487883

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487883
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences689549
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN58570.8493957644779414No Hit
CAATTACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTTTG23520.34109251119209805No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGT23260.337321930711233No Hit
GGATAATAGGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTAACATT19410.28148833512919313No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA18700.2711917499699079No Hit
ATATGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC17800.2581397406130674No Hit
CAATGACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTTTG15870.2301504316589539No Hit
ATGTCATTGGGAGTTGGGGCTCCTTGCCACAGGAACATATTGTACAAAAA15300.22188415906628822No Hit
TCCAAAGAGTATGTAAATAATGTCTCGTTTGGAAGTAATGATTAACTACA14900.2160832660188036No Hit
ATGTAATTGGGAGTTGGGGCTCCTTGCCACAGGAACATATTGTACAAAAA14650.2124577078641257No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT13440.1949100063954846No Hit
GTATGTAAATAATGTCTCGTTTGGAAGTAATGATTAACTACATGCTCTGG13120.19026929195749687No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA13120.19026929195749687No Hit
CTATTATCCAGAGCATGTAGTTAATCATTACTTCCAAACGAGACATTATT12320.17866750586252753No Hit
GTTGATAGGTGTAAGCAATTTGTGGGACCCCTAACAGTAAATGAAAACAG12230.1773623049268435No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTTTATATGCGTGTATA11900.17257656816266864No Hit
GGAACATATTGTACAAAAAATCAAATTATGTTTTAGAAAACTTCCTGTAA11880.17228652351029441No Hit
ATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGTTTGGA11790.17098132257461035No Hit
ATCCTAATGTTACCAAATACTTGCCCTTAGATAAAGGAATCAAACCCTAT11770.17069127792223612No Hit
GTGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAATTCGTTGACATACT10890.15792931321776987No Hit
GTATTTGGTAACATTAGGATAAAATCTAGCAGGCATAATTAATTTCAATC10860.15749424623920852No Hit
CCCTAACAGTAAATGAAAACAGGAGATTGAAATTAATTATGCCTGCTAGA10830.1570591792606472No Hit
CTGTTTACGCAGAAAGGCCTTATAAGTTGGCGAGAAAGTAAAAGCCTGTT10740.15575397832496313No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA10700.15517388902021464No Hit
ATCTAAGGGCAAGTATTTGGTAACATTAGGATAAAATCTAGCAGGCATAA10640.15430375506309196No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAATTTCAT10530.15270850947503367No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC10120.1467625941013619No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC10080.14618250479661343No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA9990.1448773038609294No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA9770.14168681268481284No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG9650.13994654477056742No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA9450.1370460982468251No Hit
TTCTTCTACTGTACCTAACTTTAATCCTGATTGGCAAACTCCTTCTTTTC9240.13400062939689567No Hit
ACTCACGACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT8920.12935991495890792No Hit
ACCAAATACTTGCCCTTAGATAAAGGAATCAAACCCTATTATCCAGAGCA8780.1273296023922883No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGT8690.12602440145660423No Hit
GGTGTAAGCAATTTGTGGGACCCCTAACAGTAAATGAAAACAGGAGATTG8650.12544431215185578No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA8520.12355902191142326No Hit
ATGTTAATATGGGCCTAAAGATCAGACAACTATTGTGGTTTCACATTTCC8390.12167373167099076No Hit
GTCTTACATAAGAGGACTCTTGGACTTTCAGCAATGTCAACGACCGACCT8180.1186282628210613No Hit
GTTTACAGGAAGTTTTCTAAAACATAATTTGATTTTTTGTACAATATGTT8090.11732306188537726No Hit
GTGTTGTCTCTCTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAA8010.11616288327588031No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG7960.11543777164494473No Hit
CTTTAATCCTGATTGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAGG7830.11355248140451223No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCGTGTATACAAGCTAAAC7820.1134074590783251No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAAGGGGCAGCG7780.11282736977357664No Hit
AATACGGGCCTAAAGATCAGACAACTATTGTGGTTTCACATTTCCTGTCT7770.11268234744738952No Hit
CCCTTAGATAAAGGAATCAAACCCTATTATCCAGAGCATGTAGTTAATCA7730.11210225814264106No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT7620.11050701255458277No Hit
CCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT7560.10963687859746009No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG7510.1089117669665245No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCGTGTATACAAGCTAAACAG7340.10644638742134352No Hit
GTATCAGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG7330.10630136509515639No Hit
CAGTAAATGAAAACAGGAGATTGAAATTAATTATGCCTGCTAGATTTTAT7320.10615634276896929No Hit
GCTTACACCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAA7230.10485114183328523No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA7180.10412603020234965No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT7180.10412603020234965No Hit
CAATAGGCCTGTTTACAGGAAGTTTTCTAAAACATAATTTGATTTTTTGT7160.10383598554997542No Hit
GAGTATGTAAATAATGTCTCGTTTGGAAGTAATGATTAACTACATGCTCT7150.1036909632237883No Hit
CCTTTATCTAAGGGCAAGTATTTGGTAACATTAGGATAAAATCTAGCAGG7110.10311087391903984No Hit
CGTGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTATAAGGCC7080.10267580694047848No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA7050.10224073996191714No Hit
GTTATATGGATGATATGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG6940.10064549437385885No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG6900.1000654050691104No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)