FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487885

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487885
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences338398
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG12580.37175160609696273No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT11830.3495883545411025No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10930.3229924526740702No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG10240.30260226124267875No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA9990.29521451072405863No Hit
ATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCCATGAA9960.29432798066182425No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT9660.28546268003948017No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA9490.2804390096868185No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT8980.26536799862883353No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT8880.2624128984213855No Hit
ATACAATATCATCCACATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA7810.2307933262016915No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATAATTAACCT7790.23020230616020187No Hit
TCTTTTGTCTCTGGGTATACATTTAAACCCTAACAAAACAAAAAGATGGG7510.22192802557934738No Hit
CTTTCGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC7180.21217619489476888No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT7100.20981211472881045No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT7090.20951660470806566No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7060.20863007464583125No Hit
CTCCTAGACACCGCCTCAGCTCTGTATCGAGAAGCCTTAGAGTCTCCTGA6990.2065615045006176No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTCAG6790.20065130408572154No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC6740.19917375398199752No Hit
CTTACAAGGCCTTTCTAAGTAAACAGTACATGAACCTTTACCCCGTTGCT6600.19503661369157027No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG6510.19237702350486705No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG6380.18853539323518462No Hit
TTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAG6350.1876488631729502No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTTGTGTGGGT6130.1811476427165645No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG5980.17671499240539248No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC5810.1716913220527308No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5630.16637214167932435No Hit
CTCCGAAAGAGACCAAATATTCAAGTACAGTCTCTCTTCCAAAAGTAAGG5520.1631215314511315No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT5490.1622350013888971No Hit
GTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG5420.16016643124368346No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA5390.15927990118144905No Hit
ATTCTATCCTACCCACACAAAATATTTGCCCTTAGACAAAGGAATTAAAC5300.15662031099474585No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGATCTGCGTCTGCGAGGCGA5270.15573378093251142No Hit
GTACAATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCC5090.15041460055910497No Hit
GTGTTTGATCTTTTGTACAATATGATCCTGTGGCAATGTTCCCCAACTTC5080.15011909053836014No Hit
ATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGG5080.15011909053836014No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTAT5080.15011909053836014No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA5080.15011909053836014No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACTCATAAGGTG5030.14864154043463612No Hit
GTCTAAGGGCAAATATTTTGTGTGGGTAGGATAGAATCTAGCAGGCATAA4950.1462774602686777No Hit
CTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC4900.1447999101649537No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTCCGTGAGAGGACGACAGAAT4840.14302685004048488No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTTCGCGGGACGTCCTTTGTTTA4730.13977623981229204No Hit
GTCTTACATAAGAGGACTCTTGGACTCCCAGCAATGTCAACGACCGACCT4680.138298689708568No Hit
CTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAAA4660.1377076696670784No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAAGTAGAGGACAAACGGGCAAC4630.136821139604844No Hit
ATTCCAGTACAGTCTCTCTTCCAAAAGTAAGGCAAGATATATGAAACCAC4550.13445705943888556No Hit
GTTTTAGAAAACTTCCTGTTAACAGGCCTATTGATTGGAAAGTATGTCAA4540.13416154941814076No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG4490.13268399931441677No Hit
GTAAAAGCCTGTTTAGCTTGTATACATGCATACAAAGGCATTAAGGCAGG4410.13031991914845834No Hit
ATCTAAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACTCATAAGGTG4390.12972889910696872No Hit
TTCTAAAACAGTGTTTGATCTTTTGTACAATATGATCCTGTGGCAATGTT4340.1282513490032447No Hit
GGGTAAAGGTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA4270.12618277885803109No Hit
CTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCATTTACAAG4250.12559175881654147No Hit
GGATATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAAT4190.12381869869207264No Hit
ATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATATT4130.12204563856760382No Hit
TTCAAGTACAGTCTCTCTTCCAAAAGTAAGGCAAGATATATGAAACCACA4130.12204563856760382No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTGTTTGATCTTTTGT4120.12175012854685903No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC4050.1196815584016454No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC4040.1193860483809006No Hit
CTTATAAGGCCTTTCTAAGTAAACAGTACATGAACCTTTACCCCGTTGCT4030.1190905383601558No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTGCACTCAGGCA4020.118795028339411No Hit
GTCTAGGAGATCTCTGACGGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA4000.11820400829792138No Hit
GTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA3980.11761298825643178No Hit
GTATCGAGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTG3970.11731747823568697No Hit
ATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAAC3940.11643094817345256No Hit
TTCCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTC3910.11554441811121816No Hit
GTACAGACTTGGCCCCCAATACAATATCATCCACATAGCTGAAAGCCAAA3880.11465788804898373No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC3840.11347584796600452No Hit
GTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAACAG3820.11288482792451492No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA3800.1122938078830253No Hit
CTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTT3790.11199829786228051No Hit
GGTCCTAGGAATCCTGATGTGATGTTCTCCATGTTCGTCACAGGGTCCCC3720.1099297277170669No Hit
CTCTAAGGCTTCTCGATACAGAGCTGAGGCGGTGTCTAGGAGATCTCTGA3670.10845217761334287No Hit
ACATTGACCCTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTT3630.10727013753036366No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT3610.10667911748887406No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATT3580.10579258742663963No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCCTCTACAG3560.10520156738515003No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG3550.10490605736440523No Hit
GGCCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGGGCTTT3490.10313299723993641No Hit
GGGATCTAGTAGTCAATTATGTTAATACTAACATGGGTTTAAAGATCAGG3490.10313299723993641No Hit
GTACTGTAGAGGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAA3460.10224646717770199No Hit
ATATTGTATTGGGGGCCAAGTCTGTACAGCATCGTGAGGCCCTTTATACC3430.10135993711546758No Hit
TCTCGATACAGAGCTGAGGCGGTGTCTAGGAGATCTCTGACGGAAGGAAA3430.10135993711546758No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC3420.10106442709472277No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)