FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002487888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences667446
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA50650.7588628892824288No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC23550.3528375329240119No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20760.3110363984502117No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT18050.27043386281437No Hit
CCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG17170.2572492755968273No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT16770.25125628140703515No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAAC16260.2436152138150502No Hit
CTCTATGGAAGGCGGGTATCTTATATAAGAGAGAAACAACACATAGCGCC16210.24286608954132619No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC14910.22338885842450176No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT14750.2209916607485849No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA14620.21904393763690247No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT14500.2172460393799648No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA14060.21065374577119347No Hit
GGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTT14040.21035409606170388No Hit
CACTAATATGGGCTTAAAGTTCAGGCAACTTTTGTGGTTTCACATTTCTT13920.20855619780476625No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13490.20211372905073968No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT13090.19612073486094755No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC12960.1941730117492651No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT12860.1926747632018171No Hit
CTTACAGTCAATGAAAAACGAAGACTGCAATTAATTATGCCTGCTAGGTT12830.19222528863758268No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGACGTTCACGGTGGTCTCCATGC12700.19027756552590022No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG12590.1886294921237074No Hit
ATGTTGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCC12270.18383509677187368No Hit
CATTTACGCCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT12240.18338562220763927No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT12180.18248667307917046No Hit
AATATACATTTACGCCAAGACATTATCAAAAAATGTGAACAATTTGTAGG12100.18128807424121202No Hit
CACTTACAGTCAATGAAAAACGAAGACTGCAATTAATTATGCCTGCTAGG11780.1764936788893783No Hit
CTCTTATATAAGATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT11700.1752950800514199No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGACGTTC11480.17199893324703422No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATT11110.16645541362147648No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC10980.16450769050979405No Hit
TCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGT10920.16360874138132525No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCT10710.16046241943168438No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT10580.15851469632000192No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACG10530.1577655720462779No Hit
CATCCACATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACT10410.15596767378934026No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC10260.15372030096816822No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA10250.15357047611342342No Hit
CTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTTTTGCCTTCT10180.15252170213020977No Hit
ATCCAAAGGTTACCAAATATTTGCCATTAGATAAGGGTATTAAACCTTAT10160.15222205242072018No Hit
CTAGTAAACTGAGCCATGAGAAACGGGCTGAGGCCCAATCCCATAGGAAT10050.15057397901852734No Hit
GTCTCCACCTTTGAGAAACACTCATCCTCAGGCCATGCAGTGGAATTCCA9890.14817678134261048No Hit
ATATTAGTGTTGACATAACTAACAACTAGGTCCCTAGATGCTGGATCTTC9850.14757748192363127No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTA9820.14712800735939688No Hit
GTCCCACGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAGACGGGCAAC9810.14697818250465206No Hit
CTTATATAAGATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT9620.1441315102645008No Hit
CTCATAAGGTGGGAAACTTTACAGGGCTTTATTCTTCTACTGTACCTGTC9500.14233361200756317No Hit
CCTTTGGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTTTCA9410.14098518831485993No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT9280.1390374652031775No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAATCTCCTCC9130.13679009238200543No Hit
GGTAGAGACAGCGGGGTAGGCTGCCTTCCTGACTGGCGATTGGTAGAGGC9100.13634061781777101No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC9040.1354416686893022No Hit
CTTCTAGATACCGCCTCAGCTCTCTATCGGGAAGCCTTAGAGTCTCCTGA8990.13469254441557818No Hit
CTCTAAGGCTTCCCGATAGAGAGCTGAGGCGGTATCTAGAAGATCTCGTA8960.13424306985134377No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACAGGGCTTTATTCTTCTACTG8940.13394342014185417No Hit
CTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACGCC8900.13334412072287496No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA8880.13304447101338535No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGACTCTA8740.1309469230469581No Hit
GCGTGGGACCATGCAGAACCTGCACGACTACTGTTCAAGGAACCTCTATG8690.1301977987732341No Hit
TCCTCAGGCCATGCAGTGGAATTCCACAACCTTCCACCAAACTCTGCAAG8680.13004797391848927No Hit
ATCTCGGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTA8620.12914902479002047No Hit
GGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTTTCATTGAC8570.12839990051629646No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC8380.12555322827614518No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG8290.12420480458344195No Hit
GCAATTAATTATGCCTGCTAGGTTTTATCCAAAGGTTACCAAATATTTGC8270.12390515487395236No Hit
ATATAAGATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA8120.1216577820527803No Hit
CGGTAGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTC8100.1213581323432907No Hit
GAACCACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC8090.12120830748854589No Hit
ATTAATTGCAGTCTTCGTTTTTCATTGACTGTAAGTGGGCCTACAAATTG8090.12120830748854589No Hit
TCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGA7980.11956023408635305No Hit
TGCCTAGATTGAATACAGGCATACAAGGGCATTAAAGCAGGATAACCACA7880.11806198553890501No Hit
GTGTTGTTTCTCTCTTATATAAGATACCCGCCTTCCATAGAGTGTGTAAA7880.11806198553890501No Hit
CTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCT7870.11791216068416022No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG7860.11776233582941542No Hit
GACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAATCT7680.11506548844400896No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7650.11461601387977455No Hit
GGTTTGTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT7610.11401671446079534No Hit
GGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAA7560.11326759018707132No Hit
GTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACA7490.1122188162038577No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA7420.11117004222064406No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGATGTGAT7400.11087039251115446No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC7390.11072056765640967No Hit
ATATACATTTACGCCAAGACATTATCAAAAAATGTGAACAATTTGTAGGC7380.11057074280166486No Hit
GTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA7380.11057074280166486No Hit
CTCTTTTCCTAATATACATTTACGCCAAGACATTATCAAAAAATGTGAAC7350.11012126823743044No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT7310.10952196881845123No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA7310.10952196881845123No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA7280.10907249425421682No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT7270.10892266939947202No Hit
CGCCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTTACAGT7260.10877284454472722No Hit
GGCATAATTAATTGCAGTCTTCGTTTTTCATTGACTGTAAGTGGGCCTAC7260.10877284454472722No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG7210.10802372027100318No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACAGGGCTTTATTC7210.10802372027100318No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG7190.10772407056151359No Hit
GCTTTGGGGCATGGACATCGACCCTTATAAAGAATTTGGAGCTACTGTGG7170.107424420852024No Hit
ACATAGAGGTTCCTTGAACAGTAGTCGTGCAGGTTCTGCATGGTCCCACG7140.10697494628778959No Hit
CCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG7050.10562652259508636No Hit
CTGTAAGTGGGCCTACAAATTGTTCACATTTTTTGATAATGTCTTGGCGT7050.10562652259508636No Hit
CCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGA6980.10457774861187272No Hit
AATTATGCCTGCTAGGTTTTATCCAAAGGTTACCAAATATTTGCCATTAG6950.1041282740476383No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC6900.1033791497739143No Hit
CTCCGAAAGACACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGA6860.10277985035493507No Hit
GAATAAAGCCCTGTAAAGTTTCCCACCTTATGAGTCCAAGGAATACTAAC6840.10248020064544548No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA6840.10248020064544548No Hit
CTAATATACATTTACGCCAAGACATTATCAAAAAATGTGAACAATTTGTA6830.10233037579070066No Hit
AATATGGGCTTAAAGTTCAGGCAACTTTTGTGGTTTCACATTTCTTGTCT6780.10158125151697665No Hit
CACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGT6750.10113177695274225No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAT6710.10053247753376304No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT6710.10053247753376304No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGATATTGTTTACAC6700.10038265267901822No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG6700.10038265267901822No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)