FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002487889

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487889
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences667446
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA47430.7106192860546021No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC23580.3532870074882462No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20650.3093883250480189No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAAC19440.2912595176238977No Hit
CCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG18420.2759773824399277No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA18060.27058368766911484No Hit
CTCTATGGAAGGCGGGTATCTTATATAAGAGAGAAACAACACATAGCGCC17470.2617440212391714No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT17200.25769875016106175No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT16460.2466117109099463No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT16250.24346538896030542No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT15640.2343260728208724No Hit
GGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTT14960.22413798269822577No Hit
ATGTTGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCC14020.21005444635221426No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC13630.20421127701716696No Hit
CACTAATATGGGCTTAAAGTTCAGGCAACTTTTGTGGTTTCACATTTCTT13450.20151442963176044No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA13320.19956670652007802No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT13010.1949221360229891No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC12830.19222528863758268No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12340.1848838707550873No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACG12280.18398492162661848No Hit
CTCTTATATAAGATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT11990.17964000083901918No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC11890.17814175229157117No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT11580.17349718179448223No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCT11030.16525681478351806No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTA11020.16510698992877326No Hit
CATTTACGCCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT10990.16465751536453885No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG10970.16435786565504926No Hit
ATATTAGTGTTGACATAACTAACAACTAGGTCCCTAGATGCTGGATCTTC10930.16375856623607005No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT10890.1631592668170908No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC10830.162260317688622No Hit
CTTACAGTCAATGAAAAACGAAGACTGCAATTAATTATGCCTGCTAGGTT10820.16211049283387718No Hit
GTCTCCACCTTTGAGAAACACTCATCCTCAGGCCATGCAGTGGAATTCCA10800.1618108431243876No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT10690.16016276972219476No Hit
AATATACATTTACGCCAAGACATTATCAAAAAATGTGAACAATTTGTAGG10480.15701644777255389No Hit
CTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTTTTGCCTTCT10320.15461925009663702No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATT10270.153870125822913No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG10130.15177257785648576No Hit
GTCCCACGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAGACGGGCAAC10130.15177257785648576No Hit
CACTTACAGTCAATGAAAAACGAAGACTGCAATTAATTATGCCTGCTAGG10120.15162275300174097No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA10110.15147292814699614No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA9950.1490757304710793No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGACGTTC9890.14817678134261048No Hit
TCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGT9880.14802695648786568No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC9600.14383186055501118No Hit
ATCCAAAGGTTACCAAATATTTGCCATTAGATAAGGGTATTAAACCTTAT9500.14233361200756317No Hit
CATCCACATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACT9460.14173431258858393No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGACGTTCACGGTGGTCTCCATGC9450.14158448773383914No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAATCTCCTCC9200.13783886636521905No Hit
CCTTTGGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTTTCA9190.13768904151047426No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT8940.13394342014185417No Hit
CTTATATAAGATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT8860.13274482130389575No Hit
TCCTCAGGCCATGCAGTGGAATTCCACAACCTTCCACCAAACTCTGCAAG8800.13184587217542693No Hit
GGTAGAGACAGCGGGGTAGGCTGCCTTCCTGACTGGCGATTGGTAGAGGC8770.13139639761119254No Hit
GAACCACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC8710.13049744848272368No Hit
CTAGTAAACTGAGCCATGAGAAACGGGCTGAGGCCCAATCCCATAGGAAT8660.12974832420899968No Hit
CTTCTAGATACCGCCTCAGCTCTCTATCGGGAAGCCTTAGAGTCTCCTGA8550.12810025080680684No Hit
CTCCGAAAGACACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGA8510.12750095138782763No Hit
CTCTAAGGCTTCCCGATAGAGAGCTGAGGCGGTATCTAGAAGATCTCGTA8490.127201301678338No Hit
ATCTCGGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTA8460.1267518271141036No Hit
CTCATAAGGTGGGAAACTTTACAGGGCTTTATTCTTCTACTGTACCTGTC8430.12630235254986918No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACAGGGCTTTATTCTTCTACTG8410.1260027028403796No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC8310.12450445429293157No Hit
CTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACGCC8280.12405497972869715No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA8170.1224069063265043No Hit
GCGTGGGACCATGCAGAACCTGCACGACTACTGTTCAAGGAACCTCTATG8150.12210725661701471No Hit
GGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAA8020.12015953350533227No Hit
GGTTTGTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT7850.11761251097467061No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7820.1171630364104362No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA7810.1170132115556914No Hit
ATTAATTGCAGTCTTCGTTTTTCATTGACTGTAAGTGGGCCTACAAATTG7790.11671356184620178No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC7780.11656373699145699No Hit
GGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTTTCATTGAC7710.11551496300824335No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGACTCTA7690.11521531329875376No Hit
GTTTGGGACTCTCTCGTCCCCTTCTCCGTCTGCCGTTTCGACCGACCACG7600.11386688960605053No Hit
CTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAGACC7580.11356723989656091No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA7580.11356723989656091No Hit
GCAATTAATTATGCCTGCTAGGTTTTATCCAAAGGTTACCAAATATTTGC7570.11341741504181611No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT7550.11311776533232652No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGATGTGAT7510.1125184659133473No Hit
ATATAAGATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA7480.11206899134911288No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG7430.11131986707538888No Hit
GTGTTGTTTCTCTCTTATATAAGATACCCGCCTTCCATAGAGTGTGTAAA7430.11131986707538888No Hit
GCTTTGGGGCATGGACATCGACCCTTATAAAGAATTTGGAGCTACTGTGG7300.10937214396370644No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC7300.10937214396370644No Hit
GACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAATCT7250.1086230196899824No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT7210.10802372027100318No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG7140.10697494628778959No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG7090.10622582201406557No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC7080.10607599715932074No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC6990.10472757346661751No Hit
CTCTTTTCCTAATATACATTTACGCCAAGACATTATCAAAAAATGTGAAC6900.1033791497739143No Hit
GTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA6890.10322932491916949No Hit
TGCCTAGATTGAATACAGGCATACAAGGGCATTAAAGCAGGATAACCACA6880.10307950006442469No Hit
GTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTGCGGT6860.10277985035493507No Hit
GGCATAATTAATTGCAGTCTTCGTTTTTCATTGACTGTAAGTGGGCCTAC6850.10263002550019028No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACAGGGCTTTATTC6780.10158125151697665No Hit
CTCTCGTCCCCTTCTCCGTCTGCCGTTTCGACCGACCACGGGGCGCACCT6750.10113177695274225No Hit
ATATACATTTACGCCAAGACATTATCAAAAAATGTGAACAATTTGTAGGC6740.10098195209799743No Hit
CTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCT6740.10098195209799743No Hit
TCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGA6730.10083212724325263No Hit
GTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACA6720.10068230238850784No Hit
CTGTAAGTGGGCCTACAAATTGTTCACATTTTTTGATAATGTCTTGGCGT6700.10038265267901822No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)