FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002487891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences497571
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT75461.5165674848413593No Hit
GTTCAACTGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA44420.8927369159376249No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT43360.8714334235717113No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA36650.7365782973686168No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT35860.7207011662657189No Hit
ACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCA32010.643325274181976No Hit
CTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA31840.6399086763497068No Hit
GGTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT31300.6290559538236754No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA28600.5747923411935181No Hit
GTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAA28380.5703708616458757No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACAGTTGAA27890.5605230208352175No Hit
GTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTTGTACAATA26200.5265580188556005No Hit
CCCTTAACTTCATGGGATATGTCATTGGAAGTTGGGGTACTTTACCGCAG26200.5265580188556005No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATGACATATCCCATGAA24440.49118618247446094No Hit
GCTTAGAGTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT22960.4614416836994118No Hit
GTACAAAACCTTCGGACGGAAACTGCACTTGTATTCCCATCCCATCATCC20320.4083839291277024No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA19940.4007468280908654No Hit
GGTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTTGTACAAT19390.3896931292217593No Hit
CTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACAGTTGAAC19370.38929117653560996No Hit
GTTCAACTGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTATAAAG19330.3884872711633114No Hit
CAATAGGTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTTGT19170.38527164967411687No Hit
GCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAGCCAGGAGAAA19120.3842667679587436No Hit
TCGTCGGGCTTTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGG18330.3683896368558457No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA18300.3677867078266217No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAATTTTCGAAAAC17440.35050274232220124No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG17280.3472871208330067No Hit
CTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACAGTTGAA16950.34065490151154304No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG16620.3340226821900794No Hit
GAGTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGT16190.3253806994378691No Hit
GTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATGACATATCCC15820.31794457474410687No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAATTTTCG15790.3173416457148829No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT15740.3163367639995096No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT15050.3024693963273583No Hit
CAAGCTTCCTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTA14880.29905279849508914No Hit
GGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTGCAGTTT14860.2986508458089398No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCGCG14770.29684205872126795No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC14660.2946313189474467No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT14120.28377859642141523No Hit
GACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAG13900.2793571168737728No Hit
GGAACATATTGTACAAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAA13300.26729853628929334No Hit
ATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTTGTACAATATGTT12810.25745069547863525No Hit
GGATATGTCATTGGAAGTTGGGGTACTTTACCGCAGGAACATATTGTACA12640.2540340976463661No Hit
GGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG12250.24619602026645446No Hit
GCACGGGACCATGCAAGACCTGCACAATTCCTGCTCAAGGAACCTCTATG11900.23916184825884143No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC11730.2357452504265723No Hit
TAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT11590.2329315816235271No Hit
TGCTTAGAGTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACA11420.22951498379125795No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT11240.2258974096159141No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC10940.2198681193236744No Hit
GTTTTTGTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATGACA10750.21604956880525594No Hit
GCACAACTCCTGCTCAAGGAACCTCTATGTTTCCATCTTGTTGCTGTACA10670.21444175806065868No Hit
GCACAATTCCTGCTCAAGGAACCTCTATGTTTCCATCTTGTTGCTGTACA10430.20961832582686693No Hit
GGTTCAACTGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAA10320.2074075860530457No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC10260.20620172799459777No Hit
TCGTAGGGCTTTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGG10130.20358903553462723No Hit
ATATTGTACAAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAAATAGA9890.19876560330083548No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9600.19293728935167043No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9550.19193240763629713No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA9530.1915304549501478No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9360.18811385711787867No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG9240.18570214100098276No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG9220.18530018831483347No Hit
GTTCAACTGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTATAAA9220.18530018831483347No Hit
CTTTCCAATCAATAGGTCTATTTACAGGCAATTTTCGAAAACATTGCTTG9130.18349140122716157No Hit
ATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCAA9020.18128066145334032No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA8910.1790699216795191No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT8760.17605527653339925No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC8740.17565332384724994No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA8690.17464844213187666No Hit
GGAATTGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG8580.17243770235805544No Hit
CCTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACAGTTGA8430.1694230572119356No Hit
TGCAAGACCTGCACAATTCCTGCTCAAGGAACCTCTATGTTTCCATCTTG8330.16741329378118902No Hit
GTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA8240.16560450669351712No Hit
GCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGCAGAGACCTGACC8240.16560450669351712No Hit
CCTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA8040.16158497983202397No Hit
CAGTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT7840.15756545297053084No Hit
GCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTT7810.15696252394130686No Hit
ATCATATTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT7790.15656057125515754No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG7780.1563595949120829No Hit
GTACAAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAAATAGACCTAT7720.15515373685363495No Hit
GTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTC7690.154550807824411No Hit
CTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTGCC7680.15434983148133632No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGG7590.15254104439366442No Hit
ATGTTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG7560.15193811536444043No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAACTGTATA7500.15073225730599252No Hit
TCCTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTG7430.1493254229044699No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG7250.1457078487291261No Hit
CCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATG7240.14550687238605145No Hit
GTAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTT7230.14530589604297678No Hit
GTTTTATACAGCAACAAGATGGAAACATAGAGGTTCCTTGAGCAGGAATT7220.14510491969990214No Hit
GTAAAGTACCCCAACTTCCAATGACATATCCCATGAAGTTAAGGGAGTAG7090.1424922272399316No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA7020.14108539283840899No Hit
CTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTG6920.13907562940766244No Hit
CCTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA6910.13887465306458777No Hit
GTATAAAACCTTCGGACGGAAACTGCACTTGTATTCCCATCCCATCATCC6870.13807074769228914No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGATGGA6860.1378697713492145No Hit
GCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGC6830.1372668423199905No Hit
CCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAAT6830.1372668423199905No Hit
TTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCT6800.13666391329076655No Hit
ATCAATAGGTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTT6790.1364629369476919No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG6740.1354580552323186No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6680.13425219717387066No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC6680.13425219717387066No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG6660.13385024448772134No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA6540.13143852837082548No Hit
CCAATCAATAGGTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTT6500.13063462299852682No Hit
GTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGG6480.13023267031237754No Hit
CAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTAGAGTGTA6460.12983071762622822No Hit
GTGAAAGCCTGCTTAGAGTGTATACATGCATATAAAGGCATCAAGGCAGG6460.12983071762622822No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT6450.12962974128315355No Hit
CACTAAACCCTGTTCCGACTACTGCCTCTCCCACATCGTCAATCTTCTCG6430.12922778859700423No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA6400.12862485956778028No Hit
CATGTATACACTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6400.12862485956778028No Hit
GACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGGGCTTT6380.12822290688163096No Hit
TGTATACACTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTT6190.1244043563632125No Hit
ACCCTGTTCCGACTACTGCCTCTCCCACATCGTCAATCTTCTCGAGGACT6160.12380142733398852No Hit
GTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTA6100.12259556927554056No Hit
CCCTCATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATG6030.12118873487401798No Hit
CCTCTATGTTTCCATCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC6010.12078678218786867No Hit
CATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGC5980.12018385315864469No Hit
GACCTGACCGTTGCCGGGCAACGGGGTAAAGGTTCAGATATTGTTTACAC5900.11857604241404744No Hit
TAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATGTCA5750.11556139726792759No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5680.11415456286640499No Hit
CAATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAATTTT5640.11335065749410637No Hit
GTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTA5620.11294870480795705No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG5600.11254675212180774No Hit
CTTCTGGACTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC5570.11194382309258376No Hit
ATCCAGTGGGGGTTGCGTCAGCAAACACTTGGCAGAGACCTGACCGTTGC5460.10973308331876255No Hit
TCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTAC5420.10892917794646391No Hit
AGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCC5400.10852722526031461No Hit
ACCCTGCACCGAACATGGAGAACACAACATCAGGATTCCTAGGACCCCTG5390.10832624891723996No Hit
GCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACA5360.10772331988801599No Hit
GCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTACCAAGGTATGTTGC5350.10752234354494132No Hit
GTTTTGTACAGCAACAAGATGGAAACATAGAGGTTCCTTGAGCAGGAATT5300.10651746182956803No Hit
GGATGATGGGATGGGAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTAC5300.10651746182956803No Hit
ACCCACGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCAC5280.10611550914341873No Hit
GTTTTATCATATTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTG5190.10430672205574681No Hit
GTACCCCAACTTCCAATGACATATCCCATGAAGTTAAGGGAGTAGCCCCA5180.10410574571267217No Hit
TTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA5170.1039047693695975No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAACTGTATAC5120.10289988765422421No Hit
CCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCA5110.10269891131114957No Hit
GTATACAGTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAC5110.10269891131114957No Hit
ATGTTCCTGCGGTAAAGTACCCCAACTTCCAATGACATATCCCATGAAGT5070.10189500593885094No Hit
CTCCCTTAACTTCATGGGATATGTCATTGGAAGTTGGGGTACTTTACCGC5060.1016940295957763No Hit
GACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG5060.1016940295957763No Hit
ACACTAAACCCTGTTCCGACTACTGCCTCTCCCACATCGTCAATCTTCTC5020.10089012422347765No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)