FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487894

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487894
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459637
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAAAAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAA16140.3511466657383979No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC15790.3435319610910349No Hit
TACCCTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG15550.3383104493328431No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCGCCGTG15010.32656204787691157No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14760.3211229731287951No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC13840.30110717805572657No Hit
ATTAACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT12240.2662970996677813No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA11990.26085802491966487No Hit
CAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGT11190.24345298572569224No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG11080.241059792836521No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA10390.22604794653171958No Hit
TATTAACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAG10370.2256128205518703No Hit
CTGTAAGAGGGCCCACAAATTGTTGACATCTATCAATAATGTCCTCCTGT10250.2230020646727744No Hit
GATTTGCCTCTGGCCAGTGATCCTTGTTGGGGTTGAAGTCCCAATCTGGA10090.21952105683397988No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT9980.2171278639448086No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT9920.21582248600526066No Hit
GTGTAAAAGGTGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9770.2125590411563908No Hit
ATATCTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA9630.2095131592974456No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9620.20929559630752095No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT9250.20124576568030858No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9200.20015795073068532No Hit
CCTAATCTCCTCCCCCGACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA9080.19754719485158942No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG8830.19210812010347295No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG8670.18862711226467843No Hit
GATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACGAGCAGGGGTCCTA8170.17774896276844554No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC8000.17405039193972635No Hit
GACAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACT7520.16360736842334278No Hit
GTATCGGGAAGCCTTAGAGTCCCCGGAACATTGTTCACCTCACCATACAG7410.16121417553417153No Hit
CCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGAAGTATGCCTCA7060.1535994708868085No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG7050.15338190789688386No Hit
ATGTAAGACCTTAGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC6790.14772527015884274No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6720.14620232922937015No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6710.14598476623944548No Hit
GGAAAATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGT6530.14206863242080164No Hit
CCTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTAT6520.14185106943087697No Hit
CCTTCTTTGATTGAATACATGCATATAAAGGCATCAAGGCAGGATAGCCA6490.141198380461103No Hit
GTTGTTAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCGCCGTG6450.1403281285014044No Hit
ATATTTGTCTGAGTTTTAGGCCCATATTAACATTGACATAGCTGACTACT6410.13945787654170574No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG6370.1385876245820071No Hit
GCCTTAGAGTCCCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA6250.1359768687029112No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6250.1359768687029112No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6200.1348890537532879No Hit
GGACTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGGAGTTCCACTGCAT5950.12944997900517147No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA5910.12857972704547285No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC5860.12749191209584956No Hit
GCGGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAAC5800.1261865341563016No Hit
ATCCCAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT5670.12335821528728105No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG5630.12248796332758242No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT5630.12248796332758242No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG5630.12248796332758242No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5550.12074745940818515No Hit
CTCCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG5540.1205298964182605No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGTGCAGCA5430.11813670352908925No Hit
GCCTTCTTTGATTGAATACATGCATATAAAGGCATCAAGGCAGGATAGCC5430.11813670352908925No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5400.11748401455931529No Hit
GAGACGGGTCGTCCGCGGGATTCAGCGCTGACGGGACGTAGACAAAGGAC5360.11661376259961666No Hit
CAATATTTGTCTGAGTTTTAGGCCCATATTAACATTGACATAGCTGACTA5270.11465569569029473No Hit
GTTCTATCCCAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC5250.1142205697104454No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG5230.1137854437305961No Hit
TCACCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA5220.11356788074067144No Hit
ACCAGGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC5200.11313275476082213No Hit
GGAGGCCCGTCTTCCAAACCTCGACAAGGCATGGGGACGAATCTTTCTGT5180.11269762878097281No Hit
GGAAGATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGT5170.11248006579104815No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5110.11117468785150021No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC5110.11117468785150021No Hit
GGGTAAGGCAGTAGTCGGAACAGGGTTTACTGTTCCGGAACTGGAGCCAC5020.10921662094217828No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT5000.10878149496232896No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC4970.10812880599255499No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC4880.10617073908323307No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA4860.10573561310338377No Hit
ACAAAAATCCTCACAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCT4840.10530048712353444No Hit
CTTCATCTGCCGTTCCGGCCGACCACGGGGCGCACCTCTCTTTACGCGGT4820.10486536114368514No Hit
TCTTACGAAAGCCCAGGATGATGGGATGGGAATACAAGTGCAGTTTCCGT4810.10464779815376048No Hit
AGTTAAGGGAGTAGCCCCAACGTTTAGTTTTATGGGGGTTCAAATGTATA4790.10421267217391116No Hit
ACCAACCTCCCATGCTGTAGCTCTTGTTCCCAAGAATATGGTGACCCACA4750.10334242021421251No Hit
CTATCAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG4730.10290729423436319No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4680.10181947928473992No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG4670.10160191629481526No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4650.10116679031496593No Hit
TACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACACGC4620.10051410134519197No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)