FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487895

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487895
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459637
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAAAAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAA15050.3274322998366102No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC14800.32199322508849376No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14710.32003515817917183No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCGCCGTG14370.31263801652173345No Hit
TACCCTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG13560.2950154143378362No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC12970.28217919793228136No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG12210.2656444106980073No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA11620.2528081942924525No Hit
CAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGT11600.2523730683126032No Hit
ATTAACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT11170.2430178597458429No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA10860.2362734070581785No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG10820.2354031550984799No Hit
GATTTGCCTCTGGCCAGTGATCCTTGTTGGGGTTGAAGTCCCAATCTGGA10770.2343153401488566No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT10580.23018164334028807No Hit
GTGTAAAAGGTGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT10520.22887626540074013No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT9740.21190635218661683No Hit
ATATCTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA9690.21081853723699356No Hit
CTGTAAGAGGGCCCACAAATTGTTGACATCTATCAATAATGTCCTCCTGT9640.20973072228737027No Hit
TATTAACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAG9420.20494433650902777No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9390.2042916475392538No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG9370.20385652155940448No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT8330.18122997060724008No Hit
GTATCGGGAAGCCTTAGAGTCCCCGGAACATTGTTCACCTCACCATACAG8160.17753139977852087No Hit
GATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACGAGCAGGGGTCCTA7820.17013425812108252No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7670.16687081327221265No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC7640.16621812430243865No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG7590.16513030935281536No Hit
ATATTTGTCTGAGTTTTAGGCCCATATTAACATTGACATAGCTGACTACT7310.15903854563492495No Hit
GGAAAATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGT6800.1479428331487674No Hit
CCTTCTTTGATTGAATACATGCATATAAAGGCATCAAGGCAGGATAGCCA6650.14467938829989752No Hit
GACAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACT6580.14315644737042493No Hit
GTTGTTAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCGCCGTG6470.14076325448125368No Hit
CTTCATCTGCCGTTCCGGCCGACCACGGGGCGCACCTCTCTTTACGCGGT6410.13945787654170574No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6380.13880518757193175No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6300.1370646836525345No Hit
CCTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTAT6220.13532417973313723No Hit
CCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGAAGTATGCCTCA6130.1333661128238153No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6110.13293098684396598No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG6080.132278297874192No Hit
CAATATTTGTCTGAGTTTTAGGCCCATATTAACATTGACATAGCTGACTA6000.13053779395479476No Hit
GCCTTAGAGTCCCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA5920.1287972900353975No Hit
ATATTGTACTTAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAAATCGA5880.12792703807569888No Hit
GCGGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAAC5800.1261865341563016No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA5790.12596897116637695No Hit
CTCCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG5780.12575140817645228No Hit
CCTAATCTCCTCCCCCGACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA5760.12531628219660296No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5710.12422846724697968No Hit
ATCCCAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT5640.12270552631750709No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC5570.12118258538803446No Hit
GCCTTCTTTGATTGAATACATGCATATAAAGGCATCAAGGCAGGATAGCC5540.1205298964182605No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5400.11748401455931529No Hit
GGGTAAGGCAGTAGTCGGAACAGGGTTTACTGTTCCGGAACTGGAGCCAC5390.11726645156939064No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5360.11661376259961666No Hit
ATGTAAGACCTTAGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC5330.11596107362984269No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG5310.11552594764999335No Hit
ACCAGGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC5260.11443813270037007No Hit
GGACTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGGAGTTCCACTGCAT5150.11204493981119884No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5120.11139225084142487No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5050.10986930991195226No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5040.10965174692202759No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGTGCAGCA5040.10965174692202759No Hit
CATTTGAACCCCCATAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT5040.10965174692202759No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG5030.10943418393210293No Hit
CTATCAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG5030.10943418393210293No Hit
GTTCTATCCCAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC5000.10878149496232896No Hit
GGAGGCCCGTCTTCCAAACCTCGACAAGGCATGGGGACGAATCTTTCTGT5000.10878149496232896No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC4980.10834636898247965No Hit
GGAAGATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGT4980.10834636898247965No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT4880.10617073908323307No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA4860.10573561310338377No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4830.10508292413360978No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG4760.10355998320413717No Hit
TCTTACGAAAGCCCAGGATGATGGGATGGGAATACAAGTGCAGTTTCCGT4720.10268973124443855No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4700.10225460526458922No Hit
GAGACGGGTCGTCCGCGGGATTCAGCGCTGACGGGACGTAGACAAAGGAC4680.10181947928473992No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC4660.1013843533048906No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG4630.10073166433511663No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)