FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002487896

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487896
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences560972
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA28790.513216345913878No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24580.43816803690736794No Hit
TAGCCACACTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT18900.3369152114544042No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT17740.3162368175238693No Hit
GTGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATACAAGCTAAGC16250.28967577704413056No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT16010.28539748864470954No Hit
GGTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT14600.26026254429811113No Hit
CTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA14160.2524190155658393No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCT13620.24279286666714206No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC13030.23227540768523208No Hit
CCCAATAAGGTTTGCTCCAGACCGGCTGCGAGCAAAACAAGCTGCTAGGA12470.2222927347532497No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC12310.21944054248696906No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATATC11420.20357522300578282No Hit
GCTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTG11160.1989404105730767No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA11070.19733605242329388No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG10950.19519690822358335No Hit
CCTTATAGGGACTGACAACTCTGTTGTCCTCTCTCGGAAATACACCTCCT10880.19394907410708556No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA10740.19145340587409No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG10140.18075768487553745No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACACTGTGTAAAA10080.1796881127756822No Hit
GATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC9800.17469677630969105No Hit
CTTCATCTGCCGTTCCGGCCGACCACGGGGCGCACCTCTCTTTACGCGGT9740.1736272042098358No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC9560.17041848791027003No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG9310.16596193749420649No Hit
GCAGACGAAGAAGGGGACGGTAGAGGCCCAAACGGCCCCGAGACGGGTCG9280.16542715144427886No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9270.16524888942763633No Hit
GTCCCAATAAGGTTTGCTCCAGACCGGCTGCGAGCAAAACAAGCTGCTAG9250.16489236539435123No Hit
GTAAAAGCCTGCTTAGCTTGTATACATGCATATAAAGGCATCAAGGCAGG9190.163822793294496No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCCGGTC9190.163822793294496No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTT9140.16293148321128328No Hit
ATATAAGAGAGAAACCACACGCAGTGCTTCATTTTGTGGGTCACCATATT9130.16275322119464072No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGACCG9120.1625749591779982No Hit
CTATATAAGAGAGAAACCACACGCAGTGCTTCATTTTGTGGGTCACCATA9100.1622184351447131No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG8880.1582966707785772No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8640.1540183823791562No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA8520.15187923817944568No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG8470.15098792809623296No Hit
CCCTATAAGGTTTGCTCCAGACCGGCTGCGAGCAAAACAAGCTGCTAGGA8390.14956183196309264No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG8350.14884878389652248No Hit
GTACTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT8350.14884878389652248No Hit
CCTTATTGGGACTGACAACTCTGTTGTCCTCTCTCGGAAATACACCTCCT8320.14831399784659482No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7970.14207482726410586No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT7960.14189656524746333No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7910.1410052551642506No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCT7830.1395791590311103No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTCACTTCAT7750.13815306289796994No Hit
GGATTAAAGATAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC7680.13690522878147215No Hit
GTATGTAAATAATGTCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG7620.1358356566816169No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTG7520.1340530365151915No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA7480.13333998844862133No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACCACA7470.1331617264319788No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG7430.13244867836540863No Hit
CTTTTACACAGTGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA7250.12923996206584284No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC7190.1281703899659876No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA7090.12638776979956218No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCCGGTCTCTGCCAAGTGTTTGCTG7030.12531819769970692No Hit
CTATCAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG6930.12353557753328151No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCAC6930.12353557753328151No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC6790.12103990930028594No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC6780.12086164728364339No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6780.12086164728364339No Hit
CTCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAG6760.1205051232503583No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACACTGTGTAAAAGGGGCAGCA6700.11943555115050307No Hit
GTTTTAGTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA6700.11943555115050307No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA6660.11872250308393288No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC6650.11854424106729035No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG6620.11800945501736272No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6560.11693988291750747No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG6480.11551378678436713No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC6430.11462247670115443No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG6290.11212680846815885No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG6230.1110572363683036No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGTCTAGT6170.10998766426844833No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6160.1098094022518058No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC6160.1098094022518058No Hit
ATCTTAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG6090.108561568135308No Hit
TATAGGGACTGACAACTCTGTTGTCCTCTCTCGGAAATACACCTCCTTCC6070.10820504410202292No Hit
GATTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA6040.10767025805209528No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTC6000.10695720998552512No Hit
GGAACATATTGTACTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAA6000.10695720998552512No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG5920.1055311138523848No Hit
GGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATATC5800.10339196965267429No Hit
CCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG5790.10321370763603174No Hit
AAGTAAAAGCCTGCTTAGCTTGTATACATGCATATAAAGGCATCAAGGCA5780.1030354456193892No Hit
CTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA5750.10250065956946158No Hit
CCCTCATAAAACCAAACGTTGGGGCTACTCCCTTCACTTCATGGGATATG5730.10214413553617649No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG5670.10107456343632125No Hit
CCTATAAGGTTTGCTCCAGACCGGCTGCGAGCAAAACAAGCTGCTAGGAG5660.1008963014196787No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACCACC5630.10036151536975108No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)