FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374341
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG24350.6504764372590766No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22380.5978506228278495No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT18460.49313326619312337No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA15080.40284125970705853No Hit
GTATAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC14870.3972314013159125No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT14520.3878816373306691No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAGATTAATTATGCCTGCTAGG12820.34246849797377255No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC11670.3117478448794014No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG10520.2810271917850302No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT10510.28076005567116613No Hit
CTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA10240.2735473805968355No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT10170.2716774277997868No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA9800.26179339158681525No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACTCATAAGGTG9310.24870372200747445No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA8690.23214128294790043No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT8570.22893564958153126No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG8320.22225724673492886No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA7800.20836616881399578No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTATAAGTTGGCGA7790.20809903270013172No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT7350.1963450436901114No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT7300.19500936312079095No Hit
ATATTATACTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGA7190.19207086586828587No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA6810.18191969354145018No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG6790.18138542131372198No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC6770.18085114908599378No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATCTTAATCTCCTTTTTTCA6730.1797826046305374No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6620.17684410737803233No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA6590.17604269903644004No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC6570.17550842680871184No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG6540.17470701846711956No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC6390.17069997675915807No Hit
CTATCAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG5990.16001453220459422No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC5890.15734317106595325No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC5850.15627462661049685No Hit
GATTAATTATGCCTGCTAGGTTTTATCCTAACCTTACCAAATATTTGCCC5790.15467180992731225No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5790.15467180992731225No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5780.15440467381344816No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA5740.1533361293579918No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT5650.1509319043332149No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAGTATAATA5650.1509319043332149No Hit
CTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5640.15066476821935082No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC5450.14558918205593296No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG5350.14291782091729197No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA5260.1405135958925151No Hit
ATCTAAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACTCATAAGGTG5180.13837650698160234No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA5050.13490373750136905No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAGTATAAT5040.13463660138750497No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA5010.13383519304591268No Hit
CAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAGT4990.13330092081818448No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4940.131965240248864No Hit
TCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTG4910.1311638319072717No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4910.1311638319072717No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAGTATAATATGTT4900.13089669579340762No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACTGGG4860.12982815133795123No Hit
AATTATGCCTGCTAGGTTTTATCCTAACCTTACCAAATATTTGCCCTTGG4810.12849247076863074No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA4800.12822533465476663No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTATAAGGCC4740.12662251797158205No Hit
GATTAAGATTAATTATGCCTGCTAGGTTTTATCCTAACCTTACCAAATAT4730.12635538185771797No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA4680.12501970128839746No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG4620.12341688460521288No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA4580.1223483401497565No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT4550.12154693180816423No Hit
CCCTAGCAGCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACCGAGT4530.12101265958043603No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT4490.11994411512497964No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACATCCGACCT4430.11834129844179504No Hit
ATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGG4430.11834129844179504No Hit
GGATAAAACCTAGCAGGCATAATTAATCTTAATCTCCTTTTTTCATTAAC4420.11807416232793096No Hit
CCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAACCTAGCAGG4380.11700561787247456No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA4330.11566993730315407No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT4320.11540280118928997No Hit
GTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAC4300.1148685289615618No Hit
GAATTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACA4290.11460139284769769No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT4280.11433425673383359No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAATTTCCCAC4280.11433425673383359No Hit
TAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT4210.11246430393678492No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG4210.11246430393678492No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4130.11032721502587213No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC4080.10899153445655164No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT4080.10899153445655164No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCT4070.10872439834268754No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC4050.10819012611495936No Hit
GTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAA3990.10658730943177477No Hit
GGATAAAGGCATTAAACCTTATTATCCTGAACATGCAGTTAATCATTACT3970.10605303720404657No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTG3940.1052516288624543No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCAGTGGAGTTACTCTCTTTT3910.10445022052086199No Hit
ATGTAAGACCTTGGGCAAGACCTGATGGGCGTTCACGGTGGTCTCCATGC3840.10258026772381332No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA3840.10258026772381332No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAGATTAATTATGCCTGCTAGGTT3820.1020459954960851No Hit
GGAACATATTATACTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAA3790.10124458715449283No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)