FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487902

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487902
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446150
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC33990.7618513952706488No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGACGTTGACATTGCTGAGAGTCCAA20390.45702118121707946No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19150.42922783817101867No Hit
ATGTAAGACCTTGGGCAAGACCTGATGGGCGTTCACGGGGGTCTCCATGC17670.3960551384063655No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG15030.3368822145018492No Hit
TCCTAGTACAAAGACCTTTAACCTCATCTCCTCCCCCAACTCCTCCCAGT13540.3034853748739213No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGTCCGACCT13100.2936232208898353No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTT12220.27389891292166313No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTCATCTCCTCC12010.2691919757928948No Hit
CCTTTAACCTCATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG10770.241398632746834No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGATGGGCGTTC10410.2333295976689454No Hit
CCCTAGCAGCCATGGGAATGAGGTGTATTTCCGAGAGAGGACAACCGAGT10270.23019163958309985No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGACGTTGACA10100.22638126190743021No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGGCCGTGTG9800.21965706600918974No Hit
GACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTCATCT9550.21405356942732265No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA9510.21315700997422393No Hit
CTTCAAAGACTGTTTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATGA9340.20934663229855427No Hit
GTACAAAGACCTTTAACCTCATCTCCTCCCCCAACTCCTCCCAGTCTTTA9130.20463969516978597No Hit
TTATTAATAGATGTCAACAATTCGTGGGCCCTCTTACAGTTAATGAAAAA8670.19432926145915053No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT8600.19276028241622772No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT8330.18670850610781128No Hit
CGGGGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGCCCG8240.18469124733833914No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG8240.18469124733833914No Hit
CCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGAAGTATGCCTCA8130.1822257088423176No Hit
CCTCTGCACGTCGCATGGAGACCCCCGTGAACGCCCATCAGGTCTTGCCC8050.18043258993612016No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGCCCGGCAGATGAGAAGGCACAG7540.16900145690911128No Hit
CTCTTATGTAAGACCTTGGGCAAGACCTGATGGGCGTTCACGGGGGTCTC7470.1674324778661885No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGGCCGTGTGCACTTCGCTTCAC7270.16294968060069484No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT7050.1580186036086518No Hit
CGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGGCAGATGAAGAAGG6820.15286338675333408No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG6500.1456909111285442No Hit
GGCCTCTACCGTCCCCTTCTTCATCTGCCGTTCCGGCCGACCACGGGGCG6370.14277709290597332No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGG6160.13807015577720497No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAACCACATTGTGTAAAA5970.133811498374986No Hit
ACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGTCCGACCTTGAGG5930.13291493892188727No Hit
CTACCGTCCCCTTCTTCATCTGCCGTTCCGGCCGACCACGGGGCGCACCT5930.13291493892188727No Hit
CTCTTGGACTCTCAGCAATGTCAACGTCCGACCTTGAGGCATACTTCAAA5880.13179423960551384No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5880.13179423960551384No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG5830.13067354028914044No Hit
CCTCAAGGTCGGACGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT5630.12619074302364675No Hit
GGTGAAGCGAAGTGCACACGGCCCGGCAGATGAGAAGGCACAGACGGGGA5420.1214838058948784No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT5410.12125966603160371No Hit
GTAGAGGCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG5390.12081138630505436No Hit
GTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG5370.12036310657850499No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA5340.11969068698868095No Hit
ATACTGCGGAGCTCCTAGCAGCTTGTTTTGCTCGCAGCCGGTCTGGAGCG5300.1187941275355822No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA5240.1174492883559341No Hit
ACCTTGGGCAAGACCTGATGGGCGTTCACGGGGGTCTCCATGCGACGTGC5230.11722514849265941No Hit
CTGTAAGAGGGCCCACGAATTGTTGACATCTATTAATAATGTCCTCCTGT5140.11520788972318727No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5120.1147596099966379No Hit
GACCTGATGGGCGTTCACGGGGGTCTCCATGCGACGTGCAGAGGTGAAGC5030.11274235122716576No Hit
TCCCGATACAGAGCAGAAGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA4970.11139751204751766No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC4940.1107250924576936No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC4930.11050095259441892No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT4920.11027681273114423No Hit
CTTTTACACAATGTGGTTATCCTGCCTTGATGCCTTTATATGCATGTATA4910.11005267286786954No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAAGCGGTGTCGAGGAGATCTCGAA4830.10825955396167208No Hit
CGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGGCA4750.10646643505547461No Hit
GACGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG4720.10579401546565058No Hit
GTCTCCCCGTCTGTGCCTTCTCATCTGCCGGGCCGTGTGCACTTCGCTTC4710.10556987560237589No Hit
GAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGATGGGCGT4670.10467331614927713No Hit
CCTCTTATGTAAGACCTTGGGCAAGACCTGATGGGCGTTCACGGGGGTCT4650.10422503642272779No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGATTTCTTTCCTTCTATTCG4590.10288019724307969No Hit
CCTGATGGGCGTTCACGGGGGTCTCCATGCGACGTGCAGAGGTGAAGCGA4570.10243191751653032No Hit
CCTCATTCCCATGGCTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGG4570.10243191751653032No Hit
ACCTTGAGGCATACTTCAAAGACTGTTTGTTTAAAGACTGGGAGGAGTTG4550.10198363778998094No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT4530.10153535806343159No Hit
ATATTGTACTAAAGCTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGA4500.10086293847360753No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)