FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487903

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487903
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446150
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC29970.6717471702342261No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGACGTTGACATTGCTGAGAGTCCAA19750.4426762299674997No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19080.427658859128096No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG17710.3969516978594643No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGTCCGACCT16730.3749859912585453No Hit
ATGTAAGACCTTGGGCAAGACCTGATGGGCGTTCACGGGGGTCTCCATGC14730.33015801860360866No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGGCCGTGTG11320.2537263252269416No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGACGTTGACA11070.24812282864507454No Hit
CTTCAAAGACTGTTTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATGA10200.22862266054017705No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGATGGGCGTTC10050.2252605625910568No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA9910.22212260450521124No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTCATCTCCTCC9900.22189846464193658No Hit
TTATTAATAGATGTCAACAATTCGTGGGCCCTCTTACAGTTAATGAAAAA9360.20979491202510364No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTT9270.2077776532556315No Hit
TCCTAGTACAAAGACCTTTAACCTCATCTCCTCCCCCAACTCCTCCCAGT9180.20576039448615938No Hit
CCCTAGCAGCCATGGGAATGAGGTGTATTTCCGAGAGAGGACAACCGAGT9010.20195001681048977No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT8630.19343270200605178No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGGCCGTGTGCACTTCGCTTCAC8540.19141544323657964No Hit
CGGGGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGCCCG8050.18043258993612016No Hit
CCTCTGCACGTCGCATGGAGACCCCCGTGAACGCCCATCAGGTCTTGCCC7940.1779670514400986No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT7490.16788075759273788No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG7450.16698419813963913No Hit
CCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGAAGTATGCCTCA7450.16698419813963913No Hit
CTCTTGGACTCTCAGCAATGTCAACGTCCGACCTTGAGGCATACTTCAAA7160.16048414210467332No Hit
GACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTCATCT7130.15981172251484926No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG7010.15712204415555306No Hit
ACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGTCCGACCTTGAGG6960.15600134483917963No Hit
GGCCTCTACCGTCCCCTTCTTCATCTGCCGTTCCGGCCGACCACGGGGCG6680.1497254286674885No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGCCCGGCAGATGAGAAGGCACAG6470.14501849153872015No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT6360.14255295304269863No Hit
GTCTCCCCGTCTGTGCCTTCTCATCTGCCGGGCCGTGTGCACTTCGCTTC6300.14120811386305054No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6170.13829429564047965No Hit
GTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG6000.13448391796481005No Hit
CTACCGTCCCCTTCTTCATCTGCCGTTCCGGCCGACCACGGGGCGCACCT5880.13179423960551384No Hit
CTCTTATGTAAGACCTTGGGCAAGACCTGATGGGCGTTCACGGGGGTCTC5740.12865628151966826No Hit
CGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGGCAGATGAAGAAGG5540.1241734842541746No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAACCACATTGTGTAAAA5500.12327692480107587No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT5490.12305278493780118No Hit
CCTCAAGGTCGGACGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT5490.12305278493780118No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC5340.11969068698868095No Hit
GTACAAAGACCTTTAACCTCATCTCCTCCCCCAACTCCTCCCAGTCTTTA5290.11856998767230752No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA5240.1174492883559341No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG5200.11655272890283537No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT5160.11565616944973663No Hit
ATATTGTACTAAAGCTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGA5160.11565616944973663No Hit
CCTTTAACCTCATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG5110.11453547013336321No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGG5010.11229407150061639No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG4940.1107250924576936No Hit
CCTCATTCCCATGGCTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGG4900.10982853300459487No Hit
GGATGGGGCTTGGCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGC4730.10601815532892524No Hit
ATACTGCGGAGCTCCTAGCAGCTTGTTTTGCTCGCAGCCGGTCTGGAGCG4730.10601815532892524No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA4710.10556987560237589No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGATTTCTTTCCTTCTATTCG4710.10556987560237589No Hit
GTAGAGGCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG4680.10489745601255182No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG4670.10467331614927713No Hit
CTTTTACACAATGTGGTTATCCTGCCTTGATGCCTTTATATGCATGTATA4660.10444917628600248No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4620.10355261683290373No Hit
ACCTTGAGGCATACTTCAAAGACTGTTTGTTTAAAGACTGGGAGGAGTTG4610.10332847696962905No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA4550.10198363778998094No Hit
TCTCGGGGCCGTTTGGGCCTCTACCGTCCCCTTCTTCATCTGCCGTTCCG4510.10108707833688221No Hit
GTTTGGGCCTCTACCGTCCCCTTCTTCATCTGCCGTTCCGGCCGACCACG4510.10108707833688221No Hit
GTCAACGTCCGACCTTGAGGCATACTTCAAAGACTGTTTGTTTAAAGACT4470.10019051888378348No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)