FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487904

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487904
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences549750
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC46860.852387448840382No Hit
ATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA37840.6883128694861301No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30200.5493406093678945No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT29610.5386084583901773No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG27020.49149613460663943No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG23940.4354706684856753No Hit
ATTTACAGGCAGTTTACGAAAACATTGCTTGAGTTTTTGTATAATATGAT23470.42692132787630743No Hit
ATATTATACAAAAACTCAAGCAATGTTTTCGTAAACTGCCTGTAAATAGA22980.4180081855388813No Hit
GGATCATATTATACAAAAACTCAAGCAATGTTTTCGTAAACTGCCTGTAA22450.4083674397453388No Hit
TACTCTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG22320.4060027285129605No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTACGAAAAC21850.3974533879035925No Hit
GTCTATTTACAGGCAGTTTACGAAAACATTGCTTGAGTTTTTGTATAATA21850.3974533879035925No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACCACC21460.39035925420645745No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC21000.3819918144611187No Hit
GTATACATGCATATAAAGGCATCAGAGCAGGATAGCCACATTGCGTAAAA20800.37835379718053663No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAATCCTAATAAA19780.359799909049568No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC19760.35943610732150977No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGATTCAAATGTATA18870.34324693042291954No Hit
CATTTGAATCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT18250.33196907685311505No Hit
GGTCTATTTACAGGCAGTTTACGAAAACATTGCTTGAGTTTTTGTATAAT17510.31850841291496135No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG17450.31741700773078674No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA17310.31487039563437924No Hit
CAATAGGTCTATTTACAGGCAGTTTACGAAAACATTGCTTGAGTTTTTGT16740.30450204638472034No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG16180.2943155979990905No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG15980.2906775807185084No Hit
GTATACATTTGAATCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAC15930.2897680763983629No Hit
ATATAGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC15810.28758526603001366No Hit
GTACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG15270.277762619372442No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC15200.27648931332423826No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA15140.2753979081400637No Hit
GTCTTTGGGTATACATTTGAATCCTAATAAAACCAAACGTTGGGGCTACT14460.26302864938608456No Hit
ACCAGAGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACCACC14320.2604820372896771No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA14280.2597544338335607No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG13830.25156889495225104No Hit
TAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATGTAA13600.2473851750795816No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGATCATATTATACAAAAA13580.24702137335152344No Hit
CCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAATCCTAAT13400.24374715779899953No Hit
CTTTACCACAGGATCATATTATACAAAAACTCAAGCAATGTTTTCGTAAA13330.2424738517507958No Hit
GGATAGCCACATTGCGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT12950.23556161891768987No Hit
CATATAAAGGCATCAGAGCAGGATAGCCACATTGCGTAAAAGGGGCAGCA12910.23483401546157343No Hit
TACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAG12720.23137789904502049No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT12350.22464756707594363No Hit
GGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTGCAGTTT12140.22082764893133242No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG11940.21718963165075034No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTTTCGCCAACTTACAAGGCC11880.21609822646657573No Hit
GGATATGTAATTGGAAGTTGGGGTACTTTACCACAGGATCATATTATACA11650.21191450659390632No Hit
GTTTTATTAGGATTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT11490.20900409276944065No Hit
CAATTACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTA11360.20663938153706232No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGATTCAAATGTATAC11290.20536607548885857No Hit
CTTTCCAATCAATAGGTCTATTTACAGGCAGTTTACGAAAACATTGCTTG11140.20263756252842202No Hit
GGGTATACATTTGAATCCTAATAAAACCAAACGTTGGGGCTACTCCCTTA10990.19990904956798547No Hit
GTTTACGAAAACATTGCTTGAGTTTTTGTATAATATGATCCTGTGGTAAA10750.19554342883128695No Hit
CAAGCTTCCTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTA10330.18790359254206457No Hit
GTCCCGTACTGGTGGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC10270.18681218735788996No Hit
GCGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT10270.18681218735788996No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAGAGCAGGATAGCCACAT10270.18681218735788996No Hit
ATTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA10110.18390177353342427No Hit
GATTGTATACATGCATATAAAGGCATCAGAGCAGGATAGCCACATTGCGT9000.1637107776261937No Hit
ATACTCTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAG8830.16061846293769896No Hit
ACGAACCACTGAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGAC8560.15570713960891314No Hit
ATCCTGCTCTGATGCCTTTATATGCATGTATACAATCTAAGCAGGCTTTC8280.15061391541609823No Hit
CCAATCAATAGGTCTATTTACAGGCAGTTTACGAAAACATTGCTTGAGTT8200.1491587085038654No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC8170.1486130059117781No Hit
TTTGAATCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGG8080.14697589813551615No Hit
CTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAGGATCATA8080.14697589813551615No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT8080.14697589813551615No Hit
CCTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA7950.1446111869031378No Hit
GCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA7940.1444292860391087No Hit
GTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTC7870.14315597999090496No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC7830.14242837653478854No Hit
ATACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAG7800.1418826739427012No Hit
AATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGAC7780.14151887221464302No Hit
CCCATAGGAAGCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG7730.14060936789449752No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTACG7710.1402455661664393No Hit
CCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGACTCAAGATG7650.13915416098226466No Hit
CAATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTA7560.13751705320600274No Hit
GTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAAAAAGTGA7500.1364256480218281No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA7390.13442473851750797No Hit
ATCAATAGGTCTATTTACAGGCAGTTTACGAAAACATTGCTTGAGTTTTT7350.13369713506139155No Hit
GAGCAGGATAGCCACATTGCGTAAAAGGGGCAGCAAAGCCCAAAAGACCC7300.13278763074124603No Hit
ATCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAA7250.1318781264211005No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGG7240.1316962255570714No Hit
GTAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTT7240.1316962255570714No Hit
CATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGG7200.13096862210095497No Hit
GTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAGTAGCCCCA7180.13060482037289678No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7130.12969531605275125No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG6970.1267849022282856No Hit
GTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAGTAG6920.12587539790814006No Hit
GTTTTTGTATAATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACA6710.12205547976352887No Hit
GATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAAAAAGTGAAAGCC6700.12187357889949976No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT6610.12023647112323783No Hit
CTCCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCAC6610.12023647112323783No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6610.12023647112323783No Hit
TCCTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTG6550.11914506593906321No Hit
GTGATTCGTAGGGCTTTCCCCCACTGTTTGGCTTTCAGTTATATGGATGA6550.11914506593906321No Hit
GCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGC6550.11914506593906321No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG6400.11641655297862664No Hit
TTACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTA6400.11641655297862664No Hit
TTCGTAAACTGCCTGTAAATAGACCTATTGATTGGAAAGTATGTCAAAGA6260.1138699408822192No Hit
TGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGACTC6260.1138699408822192No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG6220.11314233742610276No Hit
GACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGGGCTTT6190.11259663483401547No Hit
GTGGCTATCCTGCTCTGATGCCTTTATATGCATGTATACAATCTAAGCAG6180.11241473396998636No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAAAAAGTGAAAGCCTGCTT6160.11205093224192816No Hit
TAATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATG6150.11186903137789905No Hit
GCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGCAGAGACCAGACC6100.11095952705775353No Hit
CTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTTTTCGCCAACT6050.11005002273760801No Hit
CCAGAGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACCACCA5980.10877671668940428No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTA5880.10695770804911324No Hit
GGTATACATTTGAATCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAA5850.10641200545702591No Hit
GCCCCAACGTTTGGTTTTATTAGGATTCAAATGTATACCCAAAGACAAAA5790.10532060027285131No Hit
CTTTTACGCAATGTGGCTATCCTGCTCTGATGCCTTTATATGCATGTATA5770.10495679854479309No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT5730.10422919508867667No Hit
CCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGATTCAA5710.10386539336061847No Hit
GCCTGTAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTC5710.10386539336061847No Hit
GGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATT5620.10222828558435652No Hit
ATACAAAAACTCAAGCAATGTTTTCGTAAACTGCCTGTAAATAGACCTAT5610.10204638472032743No Hit
CTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTGCC5540.10077307867212368No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTACTGGTGGTTGATGTTCCTGG5520.10040927694406548No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)