FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences549750
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC45280.8236471123237835No Hit
ATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA36050.6557526148249204No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN29810.5422464756707595No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT28040.510050022737608No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG26480.4816734879490678No Hit
ATATTATACAAAAACTCAAGCAATGTTTTCGTAAACTGCCTGTAAATAGA23430.426193724420191No Hit
ATTTACAGGCAGTTTACGAAAACATTGCTTGAGTTTTTGTATAATATGAT23100.4201909959072305No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC22490.4090950432014552No Hit
GTATACATGCATATAAAGGCATCAGAGCAGGATAGCCACATTGCGTAAAA22100.40200090950432016No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG22040.40090950432014555No Hit
GGATCATATTATACAAAAACTCAAGCAATGTTTTCGTAAACTGCCTGTAA21270.38690313778990454No Hit
GTCTATTTACAGGCAGTTTACGAAAACATTGCTTGAGTTTTTGTATAATA21150.38472032742155526No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC20550.373806275579809No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG19770.3596180081855389No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTACGAAAAC19250.3501591632560255No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAATCCTAATAAA18870.34324693042291954No Hit
TACTCTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG17660.3212369258753979No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGATTCAAATGTATA17400.3165075034106412No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACCACC17190.31268758526603No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA17120.3114142792178263No Hit
GTCTTTGGGTATACATTTGAATCCTAATAAAACCAAACGTTGGGGCTACT16130.293406093678945No Hit
GGTCTATTTACAGGCAGTTTACGAAAACATTGCTTGAGTTTTTGTATAAT16000.2910413824465666No Hit
ATATAGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC15700.2855843565256935No Hit
GTACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG15620.28412914961346064No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG15580.28340154615734425No Hit
CATTTGAATCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT15440.2808549340609368No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG15410.2803092314688495No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC14890.2708503865393361No Hit
CAATAGGTCTATTTACAGGCAGTTTACGAAAACATTGCTTGAGTTTTTGT14640.26630286493860844No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA14450.2628467485220555No Hit
GTATACATTTGAATCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAC14420.26230104592996817No Hit
CTTTACCACAGGATCATATTATACAAAAACTCAAGCAATGTTTTCGTAAA13750.25011368804001816No Hit
GGATAGCCACATTGCGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT12920.23501591632560256No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGATCATATTATACAAAAA12660.23028649386084582No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAGAGCAGGATAGCCACAT12200.22191905411550705No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA12040.21900864029104136No Hit
TAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATGTAA12040.21900864029104136No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT11860.2157344247385175No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG11680.21246020918599365No Hit
CCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAATCCTAAT11680.21246020918599365No Hit
GTTTTATTAGGATTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT11670.2122783083219645No Hit
GGATATGTAATTGGAAGTTGGGGTACTTTACCACAGGATCATATTATACA11620.211368804001819No Hit
GGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTGCAGTTT11530.2097316962255571No Hit
ACCAGAGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACCACC11320.20591177808094588No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGATTCAAATGTATAC11170.20318326512050933No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG10830.19699863574351978No Hit
GCGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT10550.19190541155070487No Hit
GTTTACGAAAACATTGCTTGAGTTTTTGTATAATATGATCCTGTGGTAAA10530.19154160982264665No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTTTCGCCAACTTACAAGGCC10470.19045020463847204No Hit
CATATAAAGGCATCAGAGCAGGATAGCCACATTGCGTAAAAGGGGCAGCA10410.1893587994542974No Hit
CTTTCCAATCAATAGGTCTATTTACAGGCAGTTTACGAAAACATTGCTTG10170.1849931787175989No Hit
TACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAG10030.18244656662119146No Hit
GTCCCGTACTGGTGGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC9880.17971805366075488No Hit
GGGTATACATTTGAATCCTAATAAAACCAAACGTTGGGGCTACTCCCTTA9560.17389722601182356No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT9260.16844020009095043No Hit
CAATTACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTA9220.167712596634834No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC9050.16462028194633924No Hit
GTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAGTAGCCCCA8610.15661664392905866No Hit
CAAGCTTCCTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTA8440.1535243292405639No Hit
ATTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA8420.15316052751250567No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC8410.15297862664847658No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT8330.15152341973624375No Hit
GATTGTATACATGCATATAAAGGCATCAGAGCAGGATAGCCACATTGCGT8240.1498863119599818No Hit
GTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTC8220.1495225102319236No Hit
ATCCTGCTCTGATGCCTTTATATGCATGTATACAATCTAAGCAGGCTTTC7740.1407912687585266No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTACG7710.1402455661664393No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGG7650.13915416098226466No Hit
CCTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA7530.1369713506139154No Hit
ATCAATAGGTCTATTTACAGGCAGTTTACGAAAACATTGCTTGAGTTTTT7490.136243747157799No Hit
CCAATCAATAGGTCTATTTACAGGCAGTTTACGAAAACATTGCTTGAGTT7390.13442473851750797No Hit
CCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGACTCAAGATG7350.13369713506139155No Hit
TTTGAATCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGG7340.13351523419736244No Hit
CTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAGGATCATA7340.13351523419736244No Hit
CAATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTA7340.13351523419736244No Hit
CATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGG7270.1322419281491587No Hit
CTCCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCAC7260.1320600272851296No Hit
ATACTCTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAG7230.13151432469304228No Hit
CCCATAGGAAGCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG7180.13060482037289678No Hit
ACGAACCACTGAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGAC7170.13042291950886767No Hit
GTTTTTGTATAATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACA7110.12933151432469303No Hit
GCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA7100.12914961346066395No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA7050.12824010914051842No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG7000.1273306048203729No Hit
GTAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTT6860.12478399272396544No Hit
TCCTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTG6840.12442019099590725No Hit
GTGATTCGTAGGGCTTTCCCCCACTGTTTGGCTTTCAGTTATATGGATGA6820.12405638926784902No Hit
GAGCAGGATAGCCACATTGCGTAAAAGGGGCAGCAAAGCCCAAAAGACCC6810.12387448840381991No Hit
GTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAAAAAGTGA6800.1236925875397908No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6650.12096407457935425No Hit
AATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGAC6570.11950886766712143No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6480.11787175989085948No Hit
GCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGC6480.11787175989085948No Hit
CTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTTTTCGCCAACT6460.11750795816280128No Hit
ATACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAG6370.11587085038653935No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG6100.11095952705775353No Hit
GACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGGGCTTT6060.1102319236016371No Hit
ATCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAA6020.10950432014552068No Hit
GCCTGTAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTC5960.10841291496134606No Hit
GTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAGTAG5900.10732150977717143No Hit
GCCCCAACGTTTGGTTTTATTAGGATTCAAATGTATACCCAAAGACAAAA5870.10677580718508413No Hit
CTTTTACGCAATGTGGCTATCCTGCTCTGATGCCTTTATATGCATGTATA5850.10641200545702591No Hit
GGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATT5820.1058663028649386No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG5800.1055025011368804No Hit
TTACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTA5760.10477489768076398No Hit
GCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGCAGAGACCAGACC5720.10404729422464756No Hit
GTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTT5670.10313778990450205No Hit
TTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAATC5650.10277398817644383No Hit
GATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAAAAAGTGAAAGCC5540.10077307867212368No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAAAAAGTGAAAGCCTGCTT5520.10040927694406548No Hit
CCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGATTCAA5510.10022737608003639No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)