FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002487909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487909
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences499155
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN40830.8179823902395047No Hit
GTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT30250.606024180865663No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT28270.5663571435726378No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG26400.5288938305736695No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT24400.4888261161362703No Hit
GGGTAAAGGTTCAGGTATTGTTTACATAGAAAGGCCTTGTAAGTTGGCGA22430.4493594174154321No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC21950.43974316595045626No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTAC21390.4285242059079845No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTATGT19090.38244633430497543No Hit
CCTTTTACACAATGTGGTTATCCTGCATTAATGCCCTTGTATGCATGTAT17160.3437809898728852No Hit
GGATAACCACATTGTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAAT15820.3169356211998277No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA15760.31573358976670574No Hit
GCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTCTGG13070.26184251384840385No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC12910.2586370966934119No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC12480.250022538089371No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC12460.24962186094499705No Hit
CTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC12270.24581542807344411No Hit
TAACCACATTGTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAATACG12260.24561508950125713No Hit
GAATACATGCATACAAGGGCATTAATGCAGGATAACCACATTGTGTAAAA12110.24261001091845216No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT11930.23900391661908627No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG11840.23720086946940327No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC11690.23419579088659834No Hit
GGGTTACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCAT11690.23419579088659834No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC11440.22918732658192345No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG11410.22858631086536246No Hit
GAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCCAGAGA11360.22758461800442747No Hit
AGATCACATCATACATAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTA11290.22618224799911849No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACATAAA11270.22578157085474454No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG11250.22538089371037054No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT11230.22498021656599654No Hit
GTTTAAATGTATACCCAGAGACAAAAGAAAATTGGTAACAGCGGTAAAAA10820.21676633510632973No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCG10790.21616531938976868No Hit
CATGCATACAAGGGCATTAATGCAGGATAACCACATTGTGTAAAAGGAGC10770.21576464224539474No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA10740.21516362652883375No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT10710.21456261081227274No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC10470.2097544850797848No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC10320.2067494064969799No Hit
ATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATAC10320.2067494064969799No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACATAAAATCAAAGAATGT10170.20374432791417496No Hit
CTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTG10130.202942973625427No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTATGTATGATGTGAT10040.201139926475744No Hit
GTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACAT10020.20073924933137No Hit
ATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCCATGAA9980.19993789504262205No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG9940.19913654075387402No Hit
GGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACA9860.19753383217637807No Hit
CATCATACATAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGGC9680.19392773787701215No Hit
ACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCA9650.19332672216045116No Hit
TGTATACCCAGAGACAAAAGAAAATTGGTAACAGCGGTAAAAAGGGACTC9640.19312638358826417No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG9620.19272570644389017No Hit
GTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCC9600.19232502929951617No Hit
GACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAG9360.18751690356702827No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG9360.18751690356702827No Hit
TTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAAC8820.1766986206689305No Hit
CATTAATGCAGGATAACCACATTGTGTAAAAGGAGCAGCAAAACCCAAAA8720.17469523494706055No Hit
GTCTCTGGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACT8550.1712894792198816No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC8320.1666816920595807No Hit
GCCTATGACCAAGCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGC8310.1664813534873937No Hit
ACCCATAACATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCAT8200.16427762919333674No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC7930.15886848774428786No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC7850.15726577916679188No Hit
ATGTGGTTATCCTGCATTAATGCCCTTGTATGCATGTATTCAATCTAAGC7840.15706544059460487No Hit
GGTATTGTTTACATAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCC7660.15345934629523897No Hit
GGTTCAGGTATTGTTTACATAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG7660.15345934629523897No Hit
ACCACATTGTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAATACGTT7590.15205697628992998No Hit
GAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC7590.15205697628992998No Hit
GTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGT7570.151656299145556No Hit
GCTTAGATTGAATACATGCATACAAGGGCATTAATGCAGGATAACCACAT7560.151455960573369No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA7530.150854944856808No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAT7510.150454267712434No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA7490.15005359056806003No Hit
GATTGAATACATGCATACAAGGGCATTAATGCAGGATAACCACATTGTGT7420.14865122056275104No Hit
GACCCATAACATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCA7390.14805020484619005No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA7380.14784986627400307No Hit
TAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTAGATTGAA7210.14444411054682416No Hit
GCATTAATGCCCTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTT7200.14424377197463714No Hit
GTTTACATAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTA7180.14384309483026314No Hit
ATAACATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTT7060.1414390319640192No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA6950.13923530766996223No Hit
GTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACCGC6900.13823361480902727No Hit
GTTCAGGAACAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCA6900.13823361480902727No Hit
ATACGTTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCT6800.13623022908715732No Hit
CCTGTTAACAGGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCT6750.13522853622622233No Hit
GCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTA6660.13342548907653934No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC6570.13162244192685638No Hit
ACAATACGTTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTT6560.1314221033546694No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA6560.1314221033546694No Hit
GGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCT6550.1312217647824824No Hit
CCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA6500.13022007192154741No Hit
ATCCTGGGCTTTCGGAAAATTCCTATGGGAGTGGGCCTCAGCCCGTTTCT6460.12941871763279944No Hit
GTTCAGGTATTGTTTACATAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA6460.12941871763279944No Hit
CAATGACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTTTG6330.12681431619436848No Hit
GAACTACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC6320.1266139776221815No Hit
CTCCCATAGGAATTTTCCGAAAGCCCAGGATGATGGGATGGGAATACAGG6310.1264136390499945No Hit
TGTTTACATAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT6310.1264136390499945No Hit
GGATGTTATGGGTCATTGCCACAAGATCACATCATACATAAAATCAAAGA6090.12200619046188058No Hit
ACCCAAAAGACCCACAATACGTTGACAGACTTTCCAATCAATAGGCCTGT6090.12200619046188058No Hit
CATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACATAAAATCA6080.12180585188969358No Hit
GGTTTAAATGTATACCCAGAGACAAAAGAAAATTGGTAACAGCGGTAAAA6040.12100449760094559No Hit
CTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAA5960.11940178902344963No Hit
GGATGATGGGATGGGAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTAC5890.11799941901814066No Hit
TTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCATGG5810.11639671044064469No Hit
CTCACATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAACATG5810.11639671044064469No Hit
CCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAA5730.11479400186314873No Hit
CCACTGAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGG5720.11459366329096173No Hit
GCCCAGGATGATGGGATGGGAATACAGGTGCAATTTCCGTCCGAAGGTTT5710.11439332471877472No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5700.11419298614658772No Hit
CAATACGTTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTT5700.11419298614658772No Hit
AGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAATTT5700.11419298614658772No Hit
GCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTATCAAGGTATGTTGC5630.11279061614127876No Hit
ACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC5610.11238993899690478No Hit
GCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGCACAGACCTGGCC5570.11158858470815679No Hit
GTCTCTCACATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAA5540.11098756899159579No Hit
CATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5520.11058689184722181No Hit
ACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCGAGGATT5430.10878384469753884No Hit
CTCTCACATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAACA5430.10878384469753884No Hit
GCAACAGGAGGGATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTC5340.10698079754785587No Hit
CACTTGGCACAGACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGT5300.10617944325910789No Hit
GCATACAAGGGCATTAATGCAGGATAACCACATTGTGTAAAAGGAGCAGC5240.10497741182598593No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG5170.10357504182067694No Hit
GTCTGTCAACGTATTGTGGGTCTTTTGGGTTTTGCTGCTCCTTTTACACA5170.10357504182067694No Hit
ATGTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGC5150.10317436467630295No Hit
GTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACATAAAAT5140.10297402610411595No Hit
GTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAAACCCTAACAAAAC5130.10277368753192895No Hit
ATGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG5120.10257334895974198No Hit
GTGGTTATCCTGCATTAATGCCCTTGTATGCATGTATTCAATCTAAGCAG5070.10157165609880699No Hit
GTTAACAGGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTT5060.10137131752661999No Hit
CCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT5020.100569963237872No Hit
CATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGT5010.100369624665685No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)