FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487910

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487910
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences561443
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN91461.6290166588594033No Hit
TCCATAGAGTGTGCAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA15450.275183767541852No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA12940.23047753734573234No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT12400.22085946391708508No Hit
GTGCTGGTGCGCACACCAATTTATGCCTACAGCCTCCTAATACAAACAAC10640.18951166903853106No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC9400.16742572264682257No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC9220.1642196981706068No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8940.1592325489853823No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT8940.1592325489853823No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC8450.15050503791123943No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA8290.14765523837682543No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA8260.1471209009641228No Hit
CTCCAAAAGACACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGA8120.14462732637151057No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT7940.1414213018952948No Hit
CACTAATATGGGCCTAAAGTTCAGGCAGCTATTGTGGTTTCACATTTCTT7920.14106507695349307No Hit
ATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTACTGAAGGAAAGAAGT7800.13892772730268255No Hit
GTGTGCAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG7660.13643415271007028No Hit
CATTTAAACCCTAACAAAACAAAACGATGGGGTTACTCTTTACATTTCAT7380.13144700352484578No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG7210.12841909151953093No Hit
GTTATGTTAACACTAATATGGGCCTAAAGTTCAGGCAGCTATTGTGGTTT7180.12788475410682829No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA6850.12200704256709943No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT6840.12182893009619854No Hit
CTTTTACACAATGTGGTTATCCTGCCTTAATGCCCTTGTATGCATGTATT6560.11684178091097405No Hit
GGTAAAACCTAGCAGGCATAATCAATTGCAATCTTCTTTTCTCATTGACT6190.11025161948764167No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC5910.10526447030241716No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)