FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002487911

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487911
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences561443
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22760.40538398377039164No Hit
TCCATAGAGTGTGCAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA15590.2776773421344642No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT12600.22442171333510258No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA12080.21515986484825708No Hit
GTGCTGGTGCGCACACCAATTTATGCCTACAGCCTCCTAATACAAACAAC11210.19966407987988097No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT9960.17740002101727156No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC9430.16796006005952518No Hit
GTGTGCAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG9300.1656445979378138No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA9140.16279479840339983No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9020.1606574487525893No Hit
CTCCAAAAGACACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGA9000.16030122381078757No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG8290.14765523837682543No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT8270.1472990134350237No Hit
CTTTTACACAATGTGGTTATCCTGCCTTAATGCCCTTGTATGCATGTATT7960.14177752683709655No Hit
CACTAATATGGGCCTAAAGTTCAGGCAGCTATTGTGGTTTCACATTTCTT7860.13999640212808778No Hit
CATTTAAACCCTAACAAAACAAAACGATGGGGTTACTCTTTACATTTCAT7390.1316251159957467No Hit
GTTATGTTAACACTAATATGGGCCTAAAGTTCAGGCAGCTATTGTGGTTT7350.13091266611214317No Hit
GTATAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC6910.12307571739250467No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA6880.12254137997980204No Hit
GTGTAAAAGGGGCGGCAAAGCCCAAAAGACCCACAATACGTTGACAGACT6790.12093836774169417No Hit
GGTAAAACCTAGCAGGCATAATCAATTGCAATCTTCTTTTCTCATTGACT6730.11986969291628892No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT6610.11773234326547842No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6570.11701989338187492No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC6560.11684178091097405No Hit
ATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTACTGAAGGAAAGAAGT6440.11470443126016354No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG6370.11345764396385742No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT6110.10882671972043466No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC6100.10864860724953379No Hit
TTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC5870.10455202041881366No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC5830.10383957053521017No Hit
GGGTATACATTTAAACCCTAACAAAACAAAACGATGGGGTTACTCTTTAC5740.10223655829710229No Hit
CCCTTATCCAATGGCAAATATTTGGTAACTTTTGGGTAGAACCTAGCAGG5640.10045543358809354No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)