FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487912

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487912
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374634
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC14420.38490900452174653No Hit
CTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGTTT11260.30056001323958853No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11200.2989584501139779No Hit
GTATCTTCCTGCTGGTGGCTCCAGTTCAGGAACACTCAACCCTGTTCCAA10620.2834766732330755No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACACAGTCTTTGA10560.2818751101074649No Hit
ATATGATCCTGTGGCAATGTACCCCAACTTCCAATTACATAACCCATGAA10310.2752019304174207No Hit
CTATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATA10090.26932953229018186No Hit
GTATATGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAG10070.2687956779149783No Hit
GGAAAATACAGGCCCCTGACTCTGGGATCTTGCAGAGCTTGGTGGAAAGC10070.2687956779149783No Hit
GCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGTAAATGAAAAAAG9830.26238942541253596No Hit
GTGTAAATGGAGCGGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT9180.2450391582184212No Hit
CCATTTACACAATGTGGTTACCCTGCATTAATGCCTTTGTATGCATGTAT8790.2346289979019523No Hit
GTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA8400.22421883758548342No Hit
TTATTATCCTGATCATGTAGTTAATCATTACTTTCAAACCCGACATTATT8240.2199480025838552No Hit
TCCTCAGGCCATGCAGTGGAATTCCACAGCTTTCCACCAAGCTCTGCAAG7870.21007169664258984No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC7710.20580086164096156No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCCGTCCG7700.2055339344533598No Hit
CCTATTAGCAATGTCTTCATGTAAATGAATTTTAGGAAAAGAAGGGGTTT7280.19432299257408564No Hit
GGATAATAAGGTTTAATCCCTTTGTCTAAAGGCAAATATTTTGTGCTGTT7250.19352221101128034No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCAGGAACACTCAACCCTGTTCCAA7080.18898444882205032No Hit
GTCTTTGAAGTAAGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7050.18818366725924504No Hit
GGATCATATTATACAAAAAATCAAACACTGTTTTAGAAAACTTCCTGTCA6870.18337897788241322No Hit
CTCTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGCCTTGGATGGC6720.17937507006838674No Hit
TACTAATATGGGCCTAAAGATCAGGCAATTATTGTGGTTTCATATTTCTT6510.17376959912874965No Hit
GTACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA6320.1686979825643161No Hit
GTATACATGCATACAAAGGCATTAATGCAGGGTAACCACATTGTGTAAAT6220.16602871068829844No Hit
GTGCCAGCAGTGCCTCCTCCTGCCTCCACCAATCGGCAGTCAGGAAGGCA6190.16522792912549314No Hit
GTATAATATGATCCTGTGGCAATGTACCCCAACTTCCAATTACATAACCC6150.1641602203750861No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6130.16362636599988256No Hit
ATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATATT6070.16202480287427196No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGATCACACGTG6010.16042323974866135No Hit
ACTAGATACAGCCTCAGCTCTATATCGGGAAGCCTTAGAGTCTCCTGAGC5780.15428391443382075No Hit
GCTCTATATCGGGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCA5620.15001307943219247No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCCCTGAATTTCAT5570.14867844349418366No Hit
CTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGCCTTGGATGGCTT5450.14547531724296248No Hit
CATGTATACAAGCGAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5420.14467453568015717No Hit
ATGTAAGACCTTGGGCAGGACCTGATGGGCGTTCACGGTGGTCTCCATGC5320.1420052638041395No Hit
GGAATTAGAGGACAAACGGGCAACATACCTTGATAATCCAGAAGAACCAA5250.14013677349092715No Hit
TTCTTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACCCCTTCTTTTC5020.13399744817608653No Hit
GCCTAATCATCTCTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGC4980.13292973942567946No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAGTACCACATC4920.13132817630006885No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA4910.13106124911246708No Hit
TCCAAAGAGTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACA4900.13079432192486534No Hit
CACGTGTGATCCCCCTAGAAAACTGAGAGAAGTCCACCACGAGTCTAGAC4730.12625655973563532No Hit
GTATTAACATAATTGACTACTAGATCCCTGGATGCTGGATCTTCCAAATT4730.12625655973563532No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACCGCACTCAGGCA4660.12438806942242295No Hit
CATGAAGACATTGCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGT4650.12412114223482118No Hit
CTACTAGATACAGCCTCAGCTCTATATCGGGAAGCCTTAGAGTCTCCTGA4650.12412114223482118No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGCCT4630.12358728785961766No Hit
GGCTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATACTCAAGGA4570.12198572473400705No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA4570.12198572473400705No Hit
CCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTACGA4570.12198572473400705No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG4560.12171879754640529No Hit
CTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGT4540.12118494317120175No Hit
GTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC4520.12065108879599823No Hit
GGAAGATACAGGCCCCTGACTCTGGGATCTTGCAGAGCTTGGTGGAAAGC4480.11958338004559117No Hit
ATGTGGTACTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACC4340.11584639941916643No Hit
CTCTTTGGAAGGCTGGGATTCTATATAAGAGGGAAACTACACGTAGCGCC4330.11557947223156467No Hit
TCACCCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTC4310.11504561785636114No Hit
CTCCGAAAGAGACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGG4300.11477869066875937No Hit
CTTTCGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC4300.11477869066875937No Hit
GTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACATGATCAGG4280.11424483629355585No Hit
ATTTACATACTCTTTGGAAGGCTGGGATTCTATATAAGAGGGAAACTACA4260.11371098191835231No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCGAAACAGGCTTTTACTTT4240.11317712754314879No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT4220.11264327316794524No Hit
ATATAGAGCTGAGGCTGTATCTAGTAGATCCCGGACGGAAGGAAAGAAGT4190.11184249160513995No Hit
ATACAGCCCCAAGCGGCCTCGCGAGGGGTCGTCCGCGGGATTCAGCGCCG4190.11184249160513995No Hit
GTCTTCATGTAAATGAATTTTAGGAAAAGAAGGGGTTTGCCATTCAGGAT4170.11130863722993642No Hit
CTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCTTG4160.11104171004233465No Hit
ACTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGATGGAAATTGC4150.11077478285473288No Hit
GGCCTAAAGATCAGGCAATTATTGTGGTTTCATATTTCTTGCCTTACTTT4130.11024092847952936No Hit
GCACGGGACCCTGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG4110.10970707410432581No Hit
CTTTAATCCTGAATGGCAAACCCCTTCTTTTCCTAAAATTCATTTACATG4060.10837243816631699No Hit
GGGTAACCACATTGTGTAAATGGAGCGGCAAAGCCCAAAAGACCCACAAT4040.10783858379111347No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC4030.1075716566035117No Hit
ACATAGAGTTGCCTTGAGCAGGAGTCGTGCAGGTTTTGCAGGGTCCCGTG4020.10730472941590993No Hit
GTACGGCAGACGGAGAAGGGGACGATACAGCCCCAAGCGGCCTCGCGAGG3980.10623702066550286No Hit
ATATGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAGTG3960.10570316629029933No Hit
GTCTAAAGGCAAATATTTTGTGCTGTTAGGATAAAACCTAGCAGGCATAA3940.1051693119150958No Hit
GAATACAGGTGCAATTTCCATCCGTAGGTTTTGTACAGCAACATGAGGGA3920.10463545753989227No Hit
CAATAGGACGATTGACAGGAAGTTTTCTAAAACAGTGTTTGATTTTTTGT3890.10383467597708698No Hit
GGTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA3890.10383467597708698No Hit
ATATTATACAAAAAATCAAACACTGTTTTAGAAAACTTCCTGTCAATCGT3880.1035677487894852No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC3860.10303389441428167No Hit
GGGTAAAGGTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA3840.10250004003907814No Hit
ATGATGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC3840.10250004003907814No Hit
CAGTTGGACCCTGCATTCGGAGCCAATTCAAACAATCCAGATTGGGACTT3820.10196618566387461No Hit
ACCTAGCAGCCATGGAAAGGATGTATATTTCCGAGAAAGGACGACAGAAT3800.10143233128867109No Hit
CCTCTAAGAGACAGTCATCCTCAGGCCATGCAGTGGAATTCCACAGCTTT3790.10116540410106932No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT3780.10089847691346755No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG3780.10089847691346755No Hit
GTCCCGTGCTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC3760.100364622538264No Hit
ATACTAATATGGGCCTAAAGATCAGGCAATTATTGTGGTTTCATATTTCT3750.10009769535066224No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)