FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences525274
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA35590.6775511447358902No Hit
GTGCTGGTACGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC34650.6596557225371901No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28900.5501890441940778No Hit
GTCCAAGAGTCCTCTTATGTAAGATCTTGGGCAAGATTTGGTGGGCGTTC17850.339822644943401No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCCAACA15680.2985108724208699No Hit
TCCATAGAGTGTGTAAATAGTGTCTGGTTTGAAAGTAATGATTAACTAGA14380.27376188427373144No Hit
CATTATACACAAGCTGAAAGAATGTTTTAGAAAACTCCCTGTTAACAGGC14370.27357150744183034No Hit
GTGTATAATGTGCTCTTGTGGTAAGGATCCCCAACAACCAATGACATAAC13820.2631007816872718No Hit
CTCTATGGAAGGCGGGTATTTTATATAAGAGAGAAACAACACATAGCGCC13070.2488225192946919No Hit
GTCTTTGAAGTATTGCTCAAGATCGGTCGTTGACATTGTGGAGAGTCCAA11720.22312164698804815No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGATTTCTTTCCGTCTGTACG11320.21550657371200554No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11320.21550657371200554No Hit
CTATTTACACACTCTATGGAAGGCGGGTATTTTATATAAGAGAGAAACAA10470.19932454300041502No Hit
TCCTAATACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGT10460.19913416616851395No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG9880.18809230991825218No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC9620.1831425122888245No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCAACTTTTCCCAAT9010.17152952554285952No Hit
GCTCAAGATCGGTCGTTGACATTGTGGAGAGTCCAAGAGTCCTCTTATGT9010.17152952554285952No Hit
GGGTAAAGGTTCATGTATTGTTTACATAGAAAGGCCTTGTAAGTTGGCGA8990.1711487718790574No Hit
ATTTATGCCTACAGCCTCCTAATACAAAGACCTTTAACCTAATCTCCTCC8780.16715085840913504No Hit
GTGTAAATAGTGTCTGGTTTGAAAGTAATGATTAACTAGATGTTCTGGAT8770.16696048157723398No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCAGCTACCAATCGCCAGTC8500.16182030711590523No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT8480.1614395534521031No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA8480.1614395534521031No Hit
GCTCTATATCGGGATGCGTTAGAGTCTCCTGAGCATTGTTCACCTCACCA8380.15953578513309247No Hit
GTATGGATCGGCAGAGGAGCCTGAAAGGTTCCACGCATGCGCTGATGGCC8180.15572824849507114No Hit
CATTTAAACCCTAATAAGACGAAAAGATGGGGTTATTCTCTACATTTCAT8110.1543956106717637No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT8080.15382448017606049No Hit
CATATAAGGGCATTAAAGCAGGATAACCACATTGCGTGAAAGGGGCAGCA8010.15249184235275304No Hit
GCCTAATCATCTTTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7980.15192071185704986No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG7790.14830355205092963No Hit
CTCTTGGACTCTCCACAATGTCAACGACCGATCTTGAGCAATACTTCAAA7740.1473516678914243No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC7690.14639978373191895No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTGTGCCAAGTGTTTGCTG7650.1456382764043147No Hit
ATGTAAGATCTTGGGCAAGATTTGGTGGGCGTTCACGGTGGTCTCCATGC7580.14430563858100726No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA7460.14202111659819447No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA7440.14164036293439233No Hit
CTTCTAGATACCGCCGCAGCTCTATATCGGGATGCGTTAGAGTCTCCTGA7170.13650018847306358No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT7150.13611943480926145No Hit
ACATAGAGGTTCCTTGAGCAGTAGTCATGCAGGTTCTGCATGGTCCCGTG7140.13592905797736038No Hit
CCTAATACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTC7120.13554830431355824No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGAACGG7070.13459642015405293No Hit
GTTTAATACCCTTATCTAAAGGTAAATATTTGGTAAAGTTAGGGTAAAAC7060.1344060433221519No Hit
ATCTAGAAGATCTCGTACAGACGGAAAGAAATCAGAAGGCAAAAATGAGA6980.13288302866694335No Hit
CCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAG6830.13002737618842738No Hit
CATTTACACCAAGACATCATCAACAAATGTGAACAGTTTGTAGGCCCTCT6770.12888511519702098No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT6660.12679097004610929No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG6520.12412569439949436No Hit
ATTGTGGAGAGTCCAAGAGTCCTCTTATGTAAGATCTTGGGCAAGATTTG6520.12412569439949436No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCAGTCCCGATAATGTTT6520.12412569439949436No Hit
CAATAGGCCTGTTAACAGGGAGTTTTCTAAAACATTCTTTCAGCTTGTGT6500.12374494073569223No Hit
GGATAACCACATTGCGTGAAAGGGGCAGCAAAACCCAAAAGACCCACAAT6460.12298343340808797No Hit
ATATAGAGCTGCGGCGGTATCTAGAAGATCTCGTACAGACGGAAAGAAAT6320.12031815776147306No Hit
ACATGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTGTGCCAAGTG6250.1189855199381656No Hit
TCCTCAGGCCATGCAGTGGACCTCCACAACCTTCCACCAAGCTCTGCAAG6210.11822401261056134No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG6180.11765288211485815No Hit
GTCGTTGACATTGTGGAGAGTCCAAGAGTCCTCTTATGTAAGATCTTGGG6120.11651062112345177No Hit
GTATTAAACCTTACTATCCAGAACATCTAGTTAATCATTACTTTCAAACC6100.11612986745964964No Hit
GCACGGGACCATGCAGAACCTGCATGACTACTGCTCAAGGAACCTCTATG6060.11536836013204536No Hit
GGTTTGTAGTATGCCCTGAGCCTGTGGGCTCCACCCCAAAAGGCCTCCGT6020.1146068528044411No Hit
CTTATAAAGAATTTGGAGCGACTGTGGAGTTACTCTCATTTTTGCCTTCT5900.11232233082162832No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA5900.11232233082162832No Hit
GGCCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGGGTTTT5890.11213195398972726No Hit
CTCTAACGCATCCCGATATAGAGCTGCGGCGGTATCTAGAAGATCTCGTA5830.11098969299832087No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA5830.11098969299832087No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCGACCACC5820.11079931616641982No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC5760.10965705517501341No Hit
GCTTTAATGCCCTTATATGCATGTATTCAAGCGAAACAGGCTTTCACTTT5730.10908592467931022No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC5710.10870517101550808No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5690.10832441735170596No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCACCAAATCTTGCCC5670.10794366368790384No Hit
ATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCA5650.10756291002410172No Hit
GTGTAAATGTATATTGGGAAAAGTTGGTGTTTTCCAATGAGGGTTAAAGA5500.10470725754558573No Hit
CTCCAACACCGTTTCTCTTCCAAAAGTGAGACAGGAAATGTGAAACCACA5450.1037553733860804No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC5440.10356499655417935No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG5440.10356499655417935No Hit
CCTTATGAGTCCAGGGAATACTAACATTGAGAGTCCCGAGATTGAGATCT5390.10261311239467402No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA5390.10261311239467402No Hit
GTGTTGGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5350.10185160506706976No Hit
GTAGAGAATAACCCCATCTTTTCGTCTTATTAGGGTTTAAATGTATACCC5280.1005189672437623No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)