FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences525274
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA36730.6992541035726116No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28870.5496179136983745No Hit
GTGCTGGTACGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC28320.539147187943816No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCCAACA15000.2855652478515974No Hit
GTGTATAATGTGCTCTTGTGGTAAGGATCCCCAACAACCAATGACATAAC14400.2741426379375335No Hit
CATTATACACAAGCTGAAAGAATGTTTTAGAAAACTCCCTGTTAACAGGC14370.27357150744183034No Hit
TCCATAGAGTGTGTAAATAGTGTCTGGTTTGAAAGTAATGATTAACTAGA14320.27261962328232503No Hit
GTCCAAGAGTCCTCTTATGTAAGATCTTGGGCAAGATTTGGTGGGCGTTC13330.2537723169241196No Hit
CTCTATGGAAGGCGGGTATTTTATATAAGAGAGAAACAACACATAGCGCC12440.23682877888492482No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG10470.19932454300041502No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA10340.19684964418570117No Hit
GTCTTTGAAGTATTGCTCAAGATCGGTCGTTGACATTGTGGAGAGTCCAA10330.1966592673538001No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10120.19266135388387773No Hit
CTCTTGGACTCTCCACAATGTCAACGACCGATCTTGAGCAATACTTCAAA10100.19228060022007562No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGATTTCTTTCCGTCTGTACG9940.1892345709096586No Hit
CTATTTACACACTCTATGGAAGGCGGGTATTTTATATAAGAGAGAAACAA9420.1793349756508032No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC9120.17362367069377124No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCAACTTTTCCCAAT8980.17095839504715635No Hit
GGGTAAAGGTTCATGTATTGTTTACATAGAAAGGCCTTGTAAGTTGGCGA8830.16810274256864038No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT8540.16258181444350947No Hit
GTATGGATCGGCAGAGGAGCCTGAAAGGTTCCACGCATGCGCTGATGGCC8510.1620106839478063No Hit
GTGTAAATAGTGTCTGGTTTGAAAGTAATGATTAACTAGATGTTCTGGAT8200.15610900215887327No Hit
CATTTAAACCCTAATAAGACGAAAAGATGGGGTTATTCTCTACATTTCAT8180.15572824849507114No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTGTGCCAAGTGTTTGCTG8140.15496674116746686No Hit
GCTCTATATCGGGATGCGTTAGAGTCTCCTGAGCATTGTTCACCTCACCA8140.15496674116746686No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG8050.1532533496803573No Hit
TCCTAATACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGT7790.14830355205092963No Hit
GCCTAATCATCTTTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7770.1479227983871275No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCAGCTACCAATCGCCAGTC7590.1444960154129083No Hit
CTTCTAGATACCGCCGCAGCTCTATATCGGGATGCGTTAGAGTCTCCTGA7540.14354413125340298No Hit
GTTTAATACCCTTATCTAAAGGTAAATATTTGGTAAAGTTAGGGTAAAAC7490.14259224709389767No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA7490.14259224709389767No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT7430.14144998610249127No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC7430.14144998610249127No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA7390.14068847877488702No Hit
ATCTAGAAGATCTCGTACAGACGGAAAGAAATCAGAAGGCAAAAATGAGA7250.1380232031282721No Hit
GCTCAAGATCGGTCGTTGACATTGTGGAGAGTCCAAGAGTCCTCTTATGT7100.13516755064975614No Hit
CATATAAGGGCATTAAAGCAGGATAACCACATTGCGTGAAAGGGGCAGCA6990.13307340549884442No Hit
GGTTTGTAGTATGCCCTGAGCCTGTGGGCTCCACCCCAAAAGGCCTCCGT6880.1309792603479327No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCACCAAATCTTGCCC6820.1298369993565263No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6660.12679097004610929No Hit
GGCCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGGGTTTT6660.12679097004610929No Hit
ATTTATGCCTACAGCCTCCTAATACAAAGACCTTTAACCTAATCTCCTCC6630.12621983955040605No Hit
GTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG6600.12564870905470288No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT6590.1254583322228018No Hit
CATTTACACCAAGACATCATCAACAAATGTGAACAGTTTGTAGGCCCTCT6580.12526795539090074No Hit
CTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAGACC6580.12526795539090074No Hit
ATATAGAGCTGCGGCGGTATCTAGAAGATCTCGTACAGACGGAAAGAAAT6540.12450644806329648No Hit
CTCTAACGCATCCCGATATAGAGCTGCGGCGGTATCTAGAAGATCTCGTA6530.12431607123139542No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT6370.12127004192097837No Hit
GTATTAAACCTTACTATCCAGAACATCTAGTTAATCATTACTTTCAAACC6340.1206989114252752No Hit
TCCTCAGGCCATGCAGTGGACCTCCACAACCTTCCACCAAGCTCTGCAAG6330.12050853459337413No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG6200.11803363577866027No Hit
ACATGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTGTGCCAAGTG6090.11593949062774855No Hit
ACATAGAGGTTCCTTGAGCAGTAGTCATGCAGGTTCTGCATGGTCCCGTG6090.11593949062774855No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC6060.11536836013204536No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6020.1146068528044411No Hit
GCTTTAATGCCCTTATATGCATGTATTCAAGCGAAACAGGCTTTCACTTT6020.1146068528044411No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC5990.11403572230873792No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5990.11403572230873792No Hit
CAATAGGCCTGTTAACAGGGAGTTTTCTAAAACATTCTTTCAGCTTGTGT5900.11232233082162832No Hit
GCACGGGACCATGCAGAACCTGCATGACTACTGCTCAAGGAACCTCTATG5810.11060893933451876No Hit
GTGTAAATGTATATTGGGAAAAGTTGGTGTTTTCCAATGAGGGTTAAAGA5800.11041856250261768No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCAGTCCCGATAATGTTT5790.11022818567071663No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG5780.11003780883881555No Hit
GGATAACCACATTGCGTGAAAGGGGCAGCAAAACCCAAAAGACCCACAAT5780.11003780883881555No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA5770.10984743200691449No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC5750.10946667834311236No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA5750.10946667834311236No Hit
CTCCAACACCGTTTCTCTTCCAAAAGTGAGACAGGAAATGTGAAACCACA5740.1092763015112113No Hit
CTTATAAAGAATTTGGAGCGACTGTGGAGTTACTCTCATTTTTGCCTTCT5730.10908592467931022No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC5720.10889554784740917No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCGACCACC5700.10851479418360703No Hit
ATGTAAGATCTTGGGCAAGATTTGGTGGGCGTTCACGGTGGTCTCCATGC5650.10756291002410172No Hit
GTGTTGGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5460.10394575021798148No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGAACGG5450.1037553733860804No Hit
CATACTGCGGAACTCCTAGCTGCTTGTTTTGCTCGCAGCAGGTCTGGGGC5340.1016612282351687No Hit
GTAGAGAATAACCCCATCTTTTCGTCTTATTAGGGTTTAAATGTATACCC5310.10109009773946549No Hit
ATCTTGAGCAATACTTCAAAGACTGTTTGTTTAAGGACTGGGAGGAGTTG5260.10013821357996017No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)