FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487916

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487916
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420186
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACTCATAAGGTG15070.35865069278843176No Hit
GCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA14370.34199140380688553No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT12700.30224710009376804No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12670.30153313056598746No Hit
CCTTTATCCAATGGTAAATATTTGGTAACATTTGGGTAAAACCTAGCAGG12330.29344147591780784No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCGTTGACACACT12210.2905855978066856No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG11080.26369274559361805No Hit
GTTTAATACCTTTATCCAATGGTAAATATTTGGTAACATTTGGGTAAAAC10910.25964691826952824No Hit
GGTAAATATTTGGTAACATTTGGGTAAAACCTAGCAGGCATAATCAATTG10640.25322119251950326No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTCTGAATTTCAT10260.2441775785009496No Hit
CATCTACACCAAGACATTATCAACAAATGTGAACAGTTTGTAGGCCCACT10080.23989376133426626No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC8910.21204894975082467No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT8520.2027673458896774No Hit
CTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTG8210.19538966076927838No Hit
GGATAATAAGGTTTAATACCTTTATCCAATGGTAAATATTTGGTAACATT8150.19396172171371726No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC8030.19110584360259503No Hit
ATATACATCTACACCAAGACATTATCAACAAATGTGAACAGTTTGTAGGC7920.18848795533406634No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7880.18753599596369228No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGT7690.18301418895441543No Hit
ATCTAAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACTCATAAGGTG7530.17920635147291913No Hit
GTGTAGATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGATTAAAGA7410.17635047336179693No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC7360.17516052414882935No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7290.1734945952506747No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTCTTGTGGTTTCACATTTCTT7280.1732566054080812No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAAT7280.1732566054080812No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACT7270.17301861556548767No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGATCTTCGTCTGCGAGGCGA7140.16992474761177193No Hit
CTCTATGGCAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC7100.16897278824139786No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA7050.16778283902843028No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTC6910.164450981232121No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT6700.15945319453765713No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6690.15921520469506362No Hit
GGATAAAGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT6650.15826324532468955No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA6360.1513615398894775No Hit
CCTACAAACTGTTCACATTTGTTGATAATGTCTTGGTGTAGATGTATATT6290.1496956109913229No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG6210.14779169225057476No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGCTGGATCTTC6160.14660174303760715No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6130.1458877735098266No Hit
CCATATACTTGGGAACAAGAGCTACAGCATGGGGCAGAATCTTTCCACCA6010.14303189539870437No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTCTGTC6010.14303189539870437No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTACAAG5990.14255591571351733No Hit
CATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC5980.14231792587092384No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA5970.1420799360283303No Hit
GGCATAATCAATTGCAATCTTCTTTTCTCATTAACTGTGAGTGGGCCTAC5950.1416039563431433No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCG5940.14136596650054975No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACT5910.1406519969727692No Hit
TTCTATTACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA5910.1406519969727692No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGGACAACAGAGT5820.13851008838942755No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGACTCTA5780.13755812901905345No Hit
CTATTTACACACTCTATGGCAGGCGGGTATATTATATAAGAGAGAAACAA5760.13708214933386642No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTACCTGTC5730.13636817980608587No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCT5690.1354162204357118No Hit
GTCCCGTGCTGGTGGTTGAGGATCCTGGAATTAGAGGACAAACGGGCAAC5620.1337502915375572No Hit
CTCCGAAAGACACCAAATATTCTATTACTGTTTCTCTTCCAAAAGTGAGA5570.13256034232458958No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG5520.131370393111622No Hit
CTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAATATACATCTACACC5500.13089441342643496No Hit
GTATATGGTGACCCACAAAATGAGGCGCTATGTGTTGTTTCTCTCTTATA5440.12946647437087386No Hit
CTTATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTT5410.1287525048430933No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC5340.1270865759449387No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT5290.12589662673197108No Hit
CATTTGGGTAAAACCTAGCAGGCATAATCAATTGCAATCTTCTTTTCTCA5230.12446868767640998No Hit
ACCGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG5220.12423069783381645No Hit
GGTTTAATACCTTTATCCAATGGTAAATATTTGGTAACATTTGGGTAAAA5210.12399270799122294No Hit
GTATCGGGATGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTG5140.1223267790930683No Hit
GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACCGTGG5120.12185079940788127No Hit
GTTCTGGATAATAAGGTTTAATACCTTTATCCAATGGTAAATATTTGGTA5050.12018487050972665No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATATTCTATTA5000.11899492129675904No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4890.11637703302823037No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG4870.1159010533430433No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG4820.11471110413007572No Hit
CTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGAA4740.11280718538932757No Hit
TCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGA4700.11185522601895352No Hit
GTCGTTGACATTGCAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG4630.11018929712079888No Hit
GATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGATTAAAGACAGGT4620.10995130727820537No Hit
CTCTTATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGT4610.10971331743561184No Hit
GTTATGGGTCATTGCCACAAGAACACATCACACAAAAAATCAAAGAATGT4570.10876135806523778No Hit
ATCCAATGGTAAATATTTGGTAACATTTGGGTAAAACCTAGCAGGCATAA4550.10828537838005073No Hit
GTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAATATACATCTACA4550.10828537838005073No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC4550.10828537838005073No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTC4500.10709542916708314No Hit
GTCTTGGTGTAGATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGAT4460.10614346979670908No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCTTGCTT4450.10590547995411555No Hit
GGGTAAAACCTAGCAGGCATAATCAATTGCAATCTTCTTTTCTCATTAAC4420.105191510426335No Hit
AGTATATGGTGACCCACAAAATGAGGCGCTATGTGTTGTTTCTCTCTTAT4390.10447754089855443No Hit
TCTAGGGACCTAGTAGTCAGTTATGTCAACACTAATATGGGCCTAAAGTT4350.10352558152818038No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4350.10352558152818038No Hit
CACCAAGACATTATCAACAAATGTGAACAGTTTGTAGGCCCACTCACAGT4330.10304960184299335No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4320.10281161200039982No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATA4270.10162166278743223No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4260.1013836729448387No Hit
ACCATATACTTGGGAACAAGAGCTACAGCATGGGGCAGAATCTTTCCACC4250.1011456831022452No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4240.10090769325965168No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4240.10090769325965168No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGAACACATCACACAAAAA4230.10066970341705817No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)