FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487917

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487917
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420186
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACTCATAAGGTG15530.36959822554773364No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT14140.33651763742723456No Hit
GCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA13580.32319020624199757No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG13230.31486056175122445No Hit
CCTTTATCCAATGGTAAATATTTGGTAACATTTGGGTAAAACCTAGCAGG12880.30653091726045134No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12220.2908235876492791No Hit
GTTTAATACCTTTATCCAATGGTAAATATTTGGTAACATTTGGGTAAAAC11960.2846358517418477No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCGTTGACACACT11730.2791620853621967No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTCTGAATTTCAT11060.26321676590843107No Hit
GGTAAATATTTGGTAACATTTGGGTAAAACCTAGCAGGCATAATCAATTG10580.25179325346394216No Hit
GGATAATAAGGTTTAATACCTTTATCCAATGGTAAATATTTGGTAACATT8900.2118109599082311No Hit
CATCTACACCAAGACATTATCAACAAATGTGAACAGTTTGTAGGCCCACT8740.20800312242673483No Hit
CTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTG8300.19753156935262006No Hit
ATCTAAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACTCATAAGGTG8230.1958656404544654No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT8200.19515167092668484No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT8130.19348574202853022No Hit
GTGTAGATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGATTAAAGA7850.18682202643591173No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAAT7550.17968233115810617No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACT7510.1787303717877321No Hit
ATATACATCTACACCAAGACATTATCAACAAATGTGAACAGTTTGTAGGC7450.177302432732171No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTC7390.17587449367660987No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC7330.17444655462104877No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGT7290.1734945952506747No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7220.1718286663525201No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTCTTGTGGTTTCACATTTCTT7070.16825881871361728No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGATCTTCGTCTGCGAGGCGA7010.1668308796580562No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6830.16254706249137288No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6620.15754927579690897No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA6570.1563593265839414No Hit
ACCGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG6440.15326545863022567No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTACAAG6330.15064757036169696No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACT6310.15017159067650993No Hit
CCTACAAACTGTTCACATTTGTTGATAATGTCTTGGTGTAGATGTATATT6290.1496956109913229No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTCTGTC6260.14898164146354234No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC6250.1487436516209488No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC6160.14660174303760715No Hit
TTCTATTACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA6140.14612576335242014No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG6090.14493581413945253No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6070.1444598344542655No Hit
GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACCGTGG6020.1432698852412979No Hit
CCATATACTTGGGAACAAGAGCTACAGCATGGGGCAGAATCTTTCCACCA5960.14184194618573678No Hit
GGCATAATCAATTGCAATCTTCTTTTCTCATTAACTGTGAGTGGGCCTAC5960.14184194618573678No Hit
GGATAAAGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT5950.1416039563431433No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCT5820.13851008838942755No Hit
CTCCGAAAGACACCAAATATTCTATTACTGTTTCTCTTCCAAAAGTGAGA5810.13827209854683403No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5770.13732013917645994No Hit
GTATATGGTGACCCACAAAATGAGGCGCTATGTGTTGTTTCTCTCTTATA5700.13565421027830532No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGGACAACAGAGT5590.1330363220097766No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGCTGGATCTTC5550.13208436263940254No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG5520.131370393111622No Hit
CTCTTATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGT5470.1301804438986544No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTACCTGTC5440.12946647437087386No Hit
CATTTGGGTAAAACCTAGCAGGCATAATCAATTGCAATCTTCTTTTCTCA5410.1287525048430933No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA5390.12827652515790627No Hit
GTTCTGGATAATAAGGTTTAATACCTTTATCCAATGGTAAATATTTGGTA5390.12827652515790627No Hit
CTTATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTT5270.12542064704678405No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATATTCTATTA5260.12518265720419053No Hit
GTATCGGGATGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTG5250.12494466736159701No Hit
GTCCCGTGCTGGTGGTTGAGGATCCTGGAATTAGAGGACAAACGGGCAAC5240.12470667751900348No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG5210.12399270799122294No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCG5170.12304074862084886No Hit
CTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAATATACATCTACACC5170.12304074862084886No Hit
ATCCAATGGTAAATATTTGGTAACATTTGGGTAAAACCTAGCAGGCATAA5120.12185079940788127No Hit
GGTTTAATACCTTTATCCAATGGTAAATATTTGGTAACATTTGGGTAAAA5120.12185079940788127No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG5090.12113682988010072No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT5080.12089884003750719No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG5040.11994688066713313No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA4960.11804296192638498No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGACTCTA4850.11542507365785629No Hit
CATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC4800.11423512444488869No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT4760.11328316507451464No Hit
GTCTTGGTGTAGATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGAT4750.1130451752319211No Hit
GTTATGGGTCATTGCCACAAGAACACATCACACAAAAAATCAAAGAATGT4710.11209321586154702No Hit
GATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGATTAAAGACAGGT4640.11042728696339241No Hit
GGGTAAAACCTAGCAGGCATAATCAATTGCAATCTTCTTTTCTCATTAAC4610.10971331743561184No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATA4610.10971331743561184No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTC4570.10876135806523778No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGAACACATCACACAAAAA4520.10757140885227019No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC4520.10757140885227019No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC4520.10757140885227019No Hit
TAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGT4450.10590547995411555No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4440.10566749011152204No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4370.10400156121336741No Hit
GTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAATATACATCTACA4350.10352558152818038No Hit
AGTATATGGTGACCCACAAAATGAGGCGCTATGTGTTGTTTCTCTCTTAT4350.10352558152818038No Hit
GGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGAACACATCACACA4320.10281161200039982No Hit
CACCAAGACATTATCAACAAATGTGAACAGTTTGTAGGCCCACTCACAGT4300.10233563231521278No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4300.10233563231521278No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)