FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487918

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487918
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences631944
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA89101.4099350575367438No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN41500.6567037585608851No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT30040.47535857607636123No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT28960.45826845416682493No Hit
GGATAAGGGTATTAAACCTTATTATCCTGAACATCTAGTTAATCATTACT26530.4198156798703683No Hit
CATTTACACCAAGACATTATCAAAAAATGCGAACAATTTGTAGGCCCACT25040.39623764130998945No Hit
GTATACAAGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA24360.38547719418176296No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCAGGAT23610.3736090539668072No Hit
GTATTAAACCTTATTATCCTGAACATCTAGTTAATCATTACTTCCAAACC22470.35556948084007445No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA21330.3375299077133417No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT20690.3274024280632461No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGTAAATATTTGGTTACATT20430.32328813945539475No Hit
GTTTAATACCCTTATCCAATGGTAAATATTTGGTTACATTTGGATAAAAC20140.3186991252389452No Hit
ATATTTACCATTGGATAAGGGTATTAAACCTTATTATCCTGAACATCTAG18060.2857848163761346No Hit
CCCTTATCCAATGGTAAATATTTGGTTACATTTGGATAAAACCTAGCAGG17580.2781892066385629No Hit
TTATTATCCTGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT15830.25049687947033283No Hit
ATCCAAATGTAACCAAATATTTACCATTGGATAAGGGTATTAAACCTTAT15610.24701555834061245No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGATACTCTTTACATTTCAT15560.24622434899294873No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT15420.24400896281949036No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT15330.24258478599369562No Hit
CTCTATGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA14700.23261554821313282No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC13960.2209056498677098No Hit
GTCAATGAGAAAAGAAGACTACAATTGATTATGCCTGCTAGGTTTTATCC13820.2186902636942514No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT13620.2155254263035965No Hit
CTCACAGTCAATGAGAAAAGAAGACTACAATTGATTATGCCTGCTAGGTT13450.21283531452153986No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC13430.2125188307824744No Hit
CTTGTATACAGTCTAAGCAGGCTTTCATTTTTTCGCCAACTTATAAGGCC13240.20951223526135226No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC13200.2088792677832213No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACA12680.20065069056751864No Hit
GGTAAATATTTGGTTACATTTGGATAAAACCTAGCAGGCATAATCAATTG12590.19922651374172395No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGCGAACAATTTGTAGG12420.19653640195966732No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCAGG12370.1957451926120036No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12320.19495398326433988No Hit
CAATAGGCCTGTTAACTGGAAGTTTTCGAAAACATTCTTTGATTTTCTGT11730.18561771296190802No Hit
GTGTTGTTTCTCTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAA11720.1854594710923753No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT11600.18356056865798234No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCAGGATAATAAGGTTTAATAC11290.1786550707024673No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG11190.17707265200713987No Hit
CACCAAGACATTATCAAAAAATGCGAACAATTTGTAGGCCCACTCACAGT11110.17580671705087791No Hit
CTACAATTGATTATGCCTGCTAGGTTTTATCCAAATGTAACCAAATATTT10920.1728001215297558No Hit
ACCAAATATTTACCATTGGATAAGGGTATTAAACCTTATTATCCTGAACA10790.17074297722583015No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCT10790.17074297722583015No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTATAAGTTGGCGA10580.16741989796564252No Hit
GAAAAGAAGACTACAATTGATTATGCCTGCTAGGTTTTATCCAAATGTAA10570.1672616560961098No Hit
GTTCAGGATAATAAGGTTTAATACCCTTATCCAATGGTAAATATTTGGTT10550.16694517235704429No Hit
GTTTTATCCAAATGTAACCAAATATTTACCATTGGATAAGGGTATTAAAC10500.16615396300938057No Hit
ATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAG10480.16583747927031509No Hit
CTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC10350.16378033496638944No Hit
GATTATGCCTGCTAGGTTTTATCCAAATGTAACCAAATATTTACCATTGG10350.16378033496638944No Hit
ATGTAAAGAGTATCCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATAC10180.1610902231843328No Hit
GTATTAAACCTTATTATCCTGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATTTACACACTCTATGGA10090.1596660463585381No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC9960.15760890205461245No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCTTGTATA9390.14858911549124604No Hit
GGGTATTAAACCTTATTATCCTGAACATCTAGTTAATCATTACTTCCAAA9260.1465319711873204No Hit
CTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTA9010.1425759244490018No Hit
CCATTGGATAAGGGTATTAAACCTTATTATCCTGAACATCTAGTTAATCA8940.1414682313622726No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTACCTGTC8900.14083526388414164No Hit
CTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACACC8840.13988581266694516No Hit
TGCTTAGACTGTATACAAGCATACAAGGGCATTAAAGCAGGATAACCACA8770.13877811958021596No Hit
GGCATAATCAATTGTAGTCTTCTTTTCTCATTGACTGTGAGTGGGCCTAC8380.13260668666843897No Hit
GTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACA8270.13086602610357878No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG8240.13039130049498057No Hit
GGTTTAATACCCTTATCCAATGGTAAATATTTGGTTACATTTGGATAAAA8230.13023305862544785No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA8200.1297583330168496No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCAGGATAATAA8170.12928360740825137No Hit
ATGTGTTGTTTCTCTCTTATATAATATACCCGCCTTCCATAGAGTGTGTA8130.12865063993012038No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACTTGATGGGCGTTC8120.12849239806058765No Hit
CTCTTTTCCTAATATACATTTACACCAAGACATTATCAAAAAATGCGAAC8100.12817591432152214No Hit
TTATTATCCTGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATTTACACACTCTATGGA8030.12706822123479294No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC8030.12706822123479294No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT7990.12643525375666198No Hit
ATCCAATGGTAAATATTTGGTTACATTTGGATAAAACCTAGCAGGCATAA7980.12627701188712925No Hit
CCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGAT7600.120263820844885No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC7580.1199473371058195No Hit
ACAATTGATTATGCCTGCTAGGTTTTATCCAAATGTAACCAAATATTTAC7550.11947261149722127No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC7540.11931436962768853No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA7250.11472535541123896No Hit
GCTTAGACTGTATACAAGCATACAAGGGCATTAAAGCAGGATAACCACAT7220.11425062980264074No Hit
ATTCTATGCTGGGGGGAACTAATGACTCTAGCTACCTGGGTGGGTGGTAA7200.11393414606357526No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG7170.11345942045497702No Hit
ATGTAAGACCTTGGGCAAGACTTGATGGGCGTTCACGGTGGTCTCCATGC7120.1126682111073133No Hit
CTAATATACATTTACACCAAGACATTATCAAAAAATGCGAACAATTTGTA7100.11235172736824782No Hit
ATCCAATATCCAGGGACCTAGTAGTCAGTTATGTCAACACTAATATGGGC7030.1112440342815186No Hit
ATCCTGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATTTACAC7010.11092755054245312No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA6970.11029458306432215No Hit
CTCCGAAAGACACCAAATACTCTATGACTGTTTCTCTTCCAAAAGTGAGA6950.10997809932525666No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG6920.10950337371665843No Hit
GTCTAAGCAGGCTTTCATTTTTTCGCCAACTTATAAGGCCTTTCTGTGTA6860.10855392249946197No Hit
GACTACAATTGATTATGCCTGCTAGGTTTTATCCAAATGTAACCAAATAT6840.10823743876039649No Hit
CTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCT6790.10744622941273277No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG6670.10554732697833985No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6650.10523084323927437No Hit
ATATACATTTACACCAAGACATTATCAAAAAATGCGAACAATTTGTAGGC6640.10507260136974161No Hit
CTCTAAGGCTTCCCGATACAAAGCTGAGGCGGTATCTAGAAGATCTCGTA6630.10491435950020889No Hit
TTAATACCCTTATCCAATGGTAAATATTTGGTTACATTTGGATAAAACCT6620.10475611763067613No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGATACT6580.10412315015254517No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT6580.10412315015254517No Hit
GGATAAAACCTAGCAGGCATAATCAATTGTAGTCTTCTTTTCTCATTGAC6530.10333194080488144No Hit
TCCTAATACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGT6520.10317369893534871No Hit
ATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAA6440.10190776397908675No Hit
AACTAGATGTTCAGGATAATAAGGTTTAATACCCTTATCCAATGGTAAAT6430.10174952210955401No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGT6420.10159128024002126No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG6420.10159128024002126No Hit
CCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACT6390.10111655463142305No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6320.10000886154469384No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)