FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487920

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487920
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences462786
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA16000.345732152658032No Hit
CATTTACACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGTCCACT14370.310510689605995No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT13990.3022995509803667No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13670.29538490792720606No Hit
CCTCCAGCTTACAGACCACCTAATGCCCCTATCTTATCAACACTTCCGGA13650.29495274273638356No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT13420.28998284304192434No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG12820.27701788731724813No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT12610.2724801528136115No Hit
GTCTATAAGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATAC12330.2664298401420959No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACA12300.26578159235586213No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11160.24114817647897732No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATCACAGAGAGTCCAA10780.23293703785334904No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAAC10740.23207270747170397No Hit
GAAACGGGCTGAGGCCCACTCCCATAGGAATTTTCCGAAAGCCCAGGATG10590.22883146854053493No Hit
ATCTTAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG10490.22667064258642222No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTATGTAAATAATGTCTGGT10120.21867558655620525No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT10030.2167308431975038No Hit
CTTATATAATATACCCGCCTTCCATAGAGTATGTAAATAATGTCTGGTTT9640.2083036219764643No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATT9450.20419805266365015No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA9280.20052464854165855No Hit
GCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTC9000.19447433587014298No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT8960.1936100054884979No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG8860.1914491795343852No Hit
CTCTAAGGCTTCCCGATACAGAGCTGAGGCGGTATCAAGAAGATCTCGTA8500.18367020609957949No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG8430.1821576279317006No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCTTCTTTTCCTAAT8400.1815093801454668No Hit
GCTAACGACTCTAGCTACCTGGGTGGGTGTTAATTTGGAAGATCCAGCAT8280.17891638900053156No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCATCATACTGCACTCAGGCA8220.17761989342806395No Hit
GCCCATTTCTCCTGGCTCAGTTTACTAGTGCCATTTGTTCAGTGGTTCGT8050.17394648930607234No Hit
GGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATT7950.17178566335195966No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7950.17178566335195966No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC7870.1700570025886695No Hit
GCGTGGGACCATGCAGAACCTGCACGACCACTGTTCAAGGAACCTCTATG7760.16768009403914552No Hit
GGCAATAAGTGGTGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGGT7760.16768009403914552No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG7730.1670318462529117No Hit
GTCCCACGCTGGTGGTTGAGGATCCTGGAATTAGAGGACAAACGGGCAAC7690.16616751587126663No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7520.16249411174927506No Hit
GTTCAAGGAACCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGA7440.16076545098598488No Hit
GTCTTTAACCCTCATTGGAAAACACCTTCTTTTCCTAATATACATTTACA7410.16011720319975106No Hit
TCCTTGAACAGTGGTCGTGCAGGTTCTGCATGGTCCCACGCTGGTGGTTG7340.15860462503187217No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT7250.15665988167317074No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGAACACCGTG6980.15082565159706646No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG6960.15039348640624392No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC6950.15017740381083267No Hit
CTCACCAACCTCCTGTCCTCCAACTTGTCCTGGTTATCGCTGGATGTGTC6910.14931307342918756No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA6790.14672008228425235No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTC6620.14304667816226074No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT6610.1428305955668495No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCATCTACCAATCGCCAGTC6550.14153409999438185No Hit
GAGAAACACTCATCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACC6430.13894110884944663No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCTT6420.13872502625403535No Hit
CGTCCTTTGTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCTTCT6380.13786069587239028No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC6360.13742853068156774No Hit
TCCCGATACAGAGCTGAGGCGGTATCAAGAAGATCTCGTACTGAAGGAAA6360.13742853068156774No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCTTCTTTTC6300.1361320351091001No Hit
CACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGTCCACTCACAGT6200.13397120915498742No Hit
ATCCAAATGTTACCAAATATTTGCCATTAGATAAGGGTATTAAACCTTAT6130.13245863098710853No Hit
ATATAATATACCCGCCTTCCATAGAGTATGTAAATAATGTCTGGTTTGGA6130.13245863098710853No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGACGAAACGG6110.132026465796286No Hit
CTTCTTGCCCAAGGTCTTACATAAGAGGACTCTTGGACTCTCTGTGATGT6100.1318103832008747No Hit
GTGTTCCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTG6090.13159430060546345No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTGATGTCAACGACCGACCT6090.13159430060546345No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT6060.13094605281922964No Hit
TAATATACCCGCCTTCCATAGAGTATGTAAATAATGTCTGGTTTGGAAGT6020.13008172243758456No Hit
CAAAAGACGTCCCGCGCAGGATCCAGTTGGCAGCACAGCCTAGCAGCCAT5940.1283530616742944No Hit
ATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTCACGGTGGTCTCCATGC5840.12619223572018168No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5810.1255439879339479No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC5800.1253279053385366No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG5780.12489574014771405No Hit
GTATCGGGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCATCATACTG5720.12359924457524644No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC5720.12359924457524644No Hit
GAATACAGGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA5650.12208666640736755No Hit
ACATAGAGGTTCCTTGAACAGTGGTCGTGCAGGTTCTGCATGGTCCCACG5650.12208666640736755No Hit
GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGG5630.12165450121654502No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC5590.12079017083489994No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT5570.12035800564407738No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC5560.12014192304866612No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5530.1194936752624323No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG5390.11646851892667452No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA5390.11646851892667452No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG5370.11603635373585199No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC5340.1153881059496182No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAATAAGTGGTGGGCGTTC5300.1145237755679731No Hit
CTTTAACCCTCATTGGAAAACACCTTCTTTTCCTAATATACATTTACACC5290.11430769297256184No Hit
ATGATGAGGTGAGCAATGCTCAGGAGACTCTAAGGCTTCCCGATACAGAG5260.11365944518632802No Hit
GTCTGTAAGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATAC5250.11344336259091677No Hit
AATATACATTTACACCAAGACATCATCAAAAAATGTGAACAATTTGTAGG5220.11279511480468295No Hit
GTGTAAATGTATATTAGGAAAAGAAGGTGTTTTCCAATGAGGGTTAAAGA5210.11257903220927166No Hit
CTCTTGGACTCTCTGTGATGTCAACGACCGACCTTGAGGCATACTTCAAA5190.11214686701844913No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC5190.11214686701844913No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCAGACAGAAA5140.11106645404139277No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACAATGATTAATCTAATCTCC5110.11041820625515898No Hit
CTCACAAACCTCCTGTCCTCCAACTTGTCCTGGTTATCGCTGGATGTGTC5110.11041820625515898No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC5100.1102021236597477No Hit
ATACAGAGCTGAGGCGGTATCAAGAAGATCTCGTACTGAAGGAAAGAAGT5080.10976995846892516No Hit
GGGAAATACAGGCCTCTCACCCTGGGATCTTGCAGAGTTTGGTGGAAGGT5060.10933779327810263No Hit
GGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCAGACA5050.10912171068269134No Hit
GGGCAATAAGTGGTGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGG5020.10847346289645755No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT5020.10847346289645755No Hit
CCTCAAGGTCGGTCGTTGACATCACAGAGAGTCCAAGAGTCCTCTTATGT5020.10847346289645755No Hit
ACATTGACCCTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTT5010.10825738030104627No Hit
GTCAATGAGAAACGAAGACTGCAATTAATTATGCCTGCTAGGTTTTATCC5000.108041297705635No Hit
TCCTAGTACAAAGACAATGATTAATCTAATCTCCTCCCCCAACTCCTCCC4970.1073930499194012No Hit
GTACAAAGACAATGATTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCT4970.1073930499194012No Hit
CCTCCAGCTTATAGACCACCTAATGCCCCTATCTTATCAACACTTCCGGA4970.1073930499194012No Hit
ATTTGTAGGTCCACTCACAGTCAATGAGAAACGAAGACTGCAATTAATTA4940.10674480213316738No Hit
GCTTACAGACCACCTAATGCCCCTATCTTATCAACACTTCCGGAGACTAC4940.10674480213316738No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT4920.10631263694234484No Hit
TTCCCTGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCT4920.10631263694234484No Hit
GGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAA4830.10436789358364341No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA4800.1037196457974096No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG4780.10328748060658706No Hit
GATCCATACTGCGGAACTCCTAGCCGCTTGTTTTGCTCGCAGCAGGTCTG4770.10307139801117579No Hit
GGCCAGGTCTGTGCCAAGTGTTTGCTGACGCAACCCCCACTGGCTGGGGC4740.10242315022494199No Hit
CATTTGGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTCTCA4740.10242315022494199No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG4730.10220706762953072No Hit
CCTACAAATTGTTCACATTTTTTGATGATGTCTTGGTGTAAATGTATATT4680.10112665465247436No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4650.10047840686624054No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)