FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487921

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487921
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences462786
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA15660.3383853444140488No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT14650.31656100227751055No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT13750.2971135686904962No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13650.29495274273638356No Hit
GTCTATAAGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATAC12850.2776661351034819No Hit
CCTCCAGCTTACAGACCACCTAATGCCCCTATCTTATCAACACTTCCGGA12830.2772339699126594No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG12640.2731284005998453No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAAC12550.27118365724114385No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTATGTAAATAATGTCTGGT12260.26491726197421706No Hit
CATTTACACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGTCCACT11870.2564900407531775No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACA11600.2506558106770732No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT11500.24849498472296053No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATT10790.23315312044876035No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10750.23228879006711528No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT10670.23056012930382508No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG10420.22515806441854333No Hit
CTTATATAATATACCCGCCTTCCATAGAGTATGTAAATAATGTCTGGTTT10420.22515806441854333No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG10120.21867558655620525No Hit
ATCTTAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG10020.21651476060209254No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATCACAGAGAGTCCAA10010.2162986780066813No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA9280.20052464854165855No Hit
GAAACGGGCTGAGGCCCACTCCCATAGGAATTTTCCGAAAGCCCAGGATG9090.19641907922884444No Hit
GGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATT8910.19252959251144156No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT8420.18194154533628934No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA8390.18129329755005552No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG8260.17848422380970902No Hit
CGTCCTTTGTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCTTCT8210.17740381083265266No Hit
GCGTGGGACCATGCAGAACCTGCACGACCACTGTTCAAGGAACCTCTATG8180.17675556304641885No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCATCATACTGCACTCAGGCA7970.1722178285427822No Hit
GCCCATTTCTCCTGGCTCAGTTTACTAGTGCCATTTGTTCAGTGGTTCGT7910.17092133297031456No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC7780.16811225922996806No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGAACACCGTG7570.16357452472633138No Hit
CTCACCAACCTCCTGTCCTCCAACTTGTCCTGGTTATCGCTGGATGTGTC7550.16314235953550885No Hit
GCTAACGACTCTAGCTACCTGGGTGGGTGTTAATTTGGAAGATCCAGCAT7540.1629262769400976No Hit
GCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTC7520.16249411174927506No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCTTCTTTTCCTAAT7380.15946895541351727No Hit
GTTCAAGGAACCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGA7150.15449905571905806No Hit
ATATAATATACCCGCCTTCCATAGAGTATGTAAATAATGTCTGGTTTGGA7140.15428297312364678No Hit
GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGG7050.15233822976494535No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT7000.151257816787889No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6820.14736833007048614No Hit
GTCTTTAACCCTCATTGGAAAACACCTTCTTTTCCTAATATACATTTACA6750.14585575190260724No Hit
TTCCCTGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCT6740.145639669307196No Hit
GTCCCACGCTGGTGGTTGAGGATCCTGGAATTAGAGGACAAACGGGCAAC6720.14520750411637345No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG6710.14499142152096217No Hit
CTCTAAGGCTTCCCGATACAGAGCTGAGGCGGTATCAAGAAGATCTCGTA6600.1426145129714382No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA6560.14175018258979313No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC6490.14023760442191424No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6420.13872502625403535No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC6360.13742853068156774No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT6350.13721244808615646No Hit
TCCTTGAACAGTGGTCGTGCAGGTTCTGCATGGTCCCACGCTGGTGGTTG6330.13678028289533392No Hit
CTCTCGTCCCCTTCTCCGTCTGCCGTTTCGTCCGACCACGGGGCGCACCT6200.13397120915498742No Hit
GCTTGGGACTCTCTCGTCCCCTTCTCCGTCTGCCGTTTCGTCCGACCACG6200.13397120915498742No Hit
GAGAAACACTCATCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACC6120.13224254839169725No Hit
GTGTTCCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTG6110.132026465796286No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTGATGTCAACGACCGACCT6110.132026465796286No Hit
CTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTTTTGCCTTCT6030.13029780503299582No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT6030.13029780503299582No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5990.12943347465135074No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC5950.12856914426970567No Hit
CATTTGGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTCTCA5940.1283530616742944No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCATCTACCAATCGCCAGTC5930.1281369790788831No Hit
CTCTTGGACTCTCTGTGATGTCAACGACCGACCTTGAGGCATACTTCAAA5880.12705656610182678No Hit
CCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGACCCTTAT5810.1255439879339479No Hit
GTCTGTAAGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATAC5770.1246796575523028No Hit
TAATATACCCGCCTTCCATAGAGTATGTAAATAATGTCTGGTTTGGAAGT5750.12424749236148026No Hit
GGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAA5710.12338316197983516No Hit
CTTCTTGCCCAAGGTCTTACATAAGAGGACTCTTGGACTCTCTGTGATGT5700.12316707938442391No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCTTCTTTTC5690.12295099678901263No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCTTTTGTTTA5680.12273491419360137No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC5660.12230274900277883No Hit
CTCACAAACCTCCTGTCCTCCAACTTGTCCTGGTTATCGCTGGATGTGTC5630.12165450121654502No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA5600.1210062534303112No Hit
CTTTAACCCTCATTGGAAAACACCTTCTTTTCCTAATATACATTTACACC5550.11992584045325484No Hit
ACATTGACCCTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTT5530.1194936752624323No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC5530.1194936752624323No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC5510.11906151007160977No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5480.11841326228537595No Hit
GTATCGGGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCATCATACTG5410.11690068411749709No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG5410.11690068411749709No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC5400.1166846015220858No Hit
GTGTAAATGTATATTAGGAAAAGAAGGTGTTTTCCAATGAGGGTTAAAGA5370.11603635373585199No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTA5360.11582027114044073No Hit
TCTCGGGGCCGCTTGGGACTCTCTCGTCCCCTTCTCCGTCTGCCGTTTCG5340.1153881059496182No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCTT5330.11517202335420691No Hit
ATCCAAATGTTACCAAATATTTGCCATTAGATAAGGGTATTAAACCTTAT5260.11365944518632802No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG5250.11344336259091677No Hit
CAAAAGACGTCCCGCGCAGGATCCAGTTGGCAGCACAGCCTAGCAGCCAT5220.11279511480468295No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT5220.11279511480468295No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5210.11257903220927166No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5200.11236294961386041No Hit
ACATAGAGGTTCCTTGAACAGTGGTCGTGCAGGTTCTGCATGGTCCCACG5190.11214686701844913No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA5160.11149861923221534No Hit
GGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCAGACA5080.10976995846892516No Hit
CTCCGAAAGACACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGA5060.10933779327810263No Hit
CACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGTCCACTCACAGT5030.10868954549186881No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5000.108041297705635No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT5000.108041297705635No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCAGACAGAAA4980.10760913251481247No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG4940.10674480213316738No Hit
GCTTTAATGCCCTTGTATGCCTGTATTCAATCTAAGCAGGCTTTCACTTT4910.10609655434693357No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC4880.10544830656069977No Hit
TCCCGATACAGAGCTGAGGCGGTATCAAGAAGATCTCGTACTGAAGGAAA4860.10501614136987722No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT4850.10480005877446595No Hit
ATTTGTAGGTCCACTCACAGTCAATGAGAAACGAAGACTGCAATTAATTA4830.10436789358364341No Hit
CTCTTTAAATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC4810.10393572839282088No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4810.10393572839282088No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT4760.10285531541576452No Hit
GTTATGGGTCATTGCCACAAGATCACATCAGACAGAAAATCAAAGAATGT4750.10263923282035325No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4750.10263923282035325No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4750.10263923282035325No Hit
GGCCAGGTCTGTGCCAAGTGTTTGCTGACGCAACCCCCACTGGCTGGGGC4700.1015588198432969No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA4700.1015588198432969No Hit
GTATCTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA4680.10112665465247436No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTC4660.10069448946165183No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTACCTGTC4640.10026232427082928No Hit
GATCCATACTGCGGAACTCCTAGCCGCTTGTTTTGCTCGCAGCAGGTCTG4630.10004624167541801No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)