FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487922

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487922
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461315
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC16780.3637427787954001No Hit
CTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGTGTTT15910.34488364783282577No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTCCG15360.33296120871855456No Hit
GGATAATAAGGTTTAATCCCTTTGTCTAGAGGCAAATATTTAGTGCTGTT14950.3240735722879161No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14340.3108505034520881No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA14190.3075989291481959No Hit
CCTATTAGCAATGTCTTCATGTAAATGAATTTGAGGAAAAGAAGGAGTTT12920.2800689333752425No Hit
TTCTTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACTCCTTCTTTTC12270.2659787780583766No Hit
GTGTAAATGGAGCGGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT11640.25232216598202967No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG11490.2490705916781375No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA11480.24885382005787804No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10920.2367146093233474No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAATTTCAT10570.22912760261426576No Hit
CCATTTACACAATGTGGTTACCCTGCATTAATGCCTTTGTATGCATGTAT10330.22392508372803835No Hit
GTACGGGACCCTGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG10270.22262445400648145No Hit
CTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGTGT10100.2189393364620704No Hit
CTCCGAAAGAGACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGG10050.217855478360773No Hit
ATGTGGTACTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACC10020.21720516349999458No Hit
GCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGTAAATGAAAAGAG10000.2167716202594756No Hit
ATATGATCCTGTGGCAATGTACCCCAACTTCCAATTACATATCCCATGAA9990.21655484863921615No Hit
TTACATATCCCATGAAATTTAGGGAATAACCCCATCTTTTTGTTTTGTTA9850.21352004595558352No Hit
TCCCGATACAGAGCTGAGGCTGTATCTAGTAGATCTCGGACGGAAGGAAA9630.208751070309875No Hit
GTATACATGCATACAAAGGCATTAATGCAGGGTAACCACATTGTGTAAAT9540.20680012572753975No Hit
GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGG9220.19986343387923652No Hit
TTATTATCCTGATCATGTAGTTAATCATTACTTTCAAACACGACATTATT9090.19704540281586333No Hit
GGATCATATTGTACAGAAAATCAAACATTGTTTTAGGAAACTTCCTGTCA8750.18967516772704118No Hit
GTACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCGA8750.18967516772704118No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC8300.17992044481536476No Hit
CATGTATACAAGCGAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC8170.1771024137519916No Hit
CTATATAAGAGGGAATCTACACGTAGCGCCTCATTTTGCGGGTCACCATA8010.17363406782784No Hit
CTCTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGCCTTGGATGGC7980.17298375296706153No Hit
CTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGCCTTGGATGGCTT7610.16496320301746095No Hit
TCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTG7410.16062777061227146No Hit
TCCAAAGAGTATGTAAATAATGTCGTGTTTGAAAGTAATGATTAACTACA7390.16019422737175248No Hit
CTCTAAGGCTTCCCGATACAGAGCTGAGGCTGTATCTAGTAGATCTCGGA7370.15976068413123354No Hit
GGCTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATACTCAAGGA7260.1573761963083793No Hit
CATGAAGACATTGCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGT7160.15520848010578456No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT7150.15499170848552507No Hit
GTGTAGATTCCCTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAA7150.15499170848552507No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAGTACCACATC7060.1530407639031898No Hit
CTTTAATCCTGAATGGCAAACTCCTTCTTTTCCTCAAATTCATTTACATG7000.15174013418163293No Hit
GTATCGGGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACAG6920.15000596121955714No Hit
GGCCTAAAGATCAGGCAACTATTGTGGTTTCATATTTCTTGCCTTACTTT6840.14827178825748133No Hit
CTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTGTCCCTATC6820.14783824501696238No Hit
GTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA6770.146754386915665No Hit
GTATGTAAATAATGTCGTGTTTGAAAGTAATGATTAACTACATGATCAGG6710.14545375719410814No Hit
CTCAAGGACAGTTTCTCTTCCAAAAGTAAGGCAAGAAATATGAAACCACA6660.14436989909281078No Hit
ATACTAACATGGGCCTAAAGATCAGGCAACTATTGTGGTTTCATATTTCT6610.1432860409915134No Hit
GGGTAAAGGTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA6610.1432860409915134No Hit
AAATAATGTCGTGTTTGAAAGTAATGATTAACTACATGATCAGGATAATA6540.14176863964969708No Hit
ATTTAGTGCTGTTAGGATAAAATCTAGCAGGCATAATTAATTTCAATCTT6490.1406847815483997No Hit
ACCTAGCAGCCATGGAAAAGATGTATATTTCCGAGAAAGGACGACAGAAT6430.13938415182684283No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAGCTTTCCACCAAGCTCTGCAAG6420.13916738020658337No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACAGCACTCAGGCA6390.1385170653458049No Hit
ACTAGATACAGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGAGC6210.13461517618113436No Hit
GTCTAGAGGCAAATATTTAGTGCTGTTAGGATAAAATCTAGCAGGCATAA6160.13353131807983698No Hit
GCACTAAATATTTGCCTCTAGACAAAGGGATTAAACCTTATTATCCTGAT6120.1326642315987991No Hit
ATATAAGAGGGAATCTACACGTAGCGCCTCATTTTGCGGGTCACCATATT6110.1324474599785396No Hit
GACCAGGCCGTTGCCGGGCAACGGGGTAAAGGTTCATGTACTGTTTACTT6090.13201391673802065No Hit
GTAGAAGAATAAAGCCCAGTAAAATTTCCCACCTTATGAGTCCAAGGAAT5950.128979114054388No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5940.12876234243412854No Hit
ATACAGCACTCAGGCAAGCCATTCTCTGCTGGGGGGAATTAATGACTCTA5930.12854557081386905No Hit
ATACAGAGCTGAGGCTGTATCTAGTAGATCTCGGACGGAAGGAAAGAAGT5900.12789525595309062No Hit
GTACAATATGATCCTGTGGCAATGTACCCCAACTTCCAATTACATATCCC5810.12594431137075535No Hit
TCACCATACAGCACTCAGGCAAGCCATTCTCTGCTGGGGGGAATTAATGA5670.12290950868712266No Hit
GTGCTGTATGGTGAGGTGAGCAATGCTCAGGAGACTCTAAGGCTTCCCGA5550.12030824924400896No Hit
GGGTAACCACATTGTGTAAATGGAGCGGCAAAGCCCAAAAGACCCACAAT5490.11900761952245212No Hit
GTACGGCAGACGGAGAAGGGGACGATAGAGCCCCAAGCGGCCTCGCGAGG5470.11857407628193316No Hit
ACATTGACCCTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTT5440.11792376142115474No Hit
ATCTAGTAGATCTCGGACGGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA5400.11705667494011683No Hit
GTCCCTATCTTTAATCCTGAATGGCAAACTCCTTCTTTTCCTCAAATTCA5370.11640636007933841No Hit
ATATTGTACAGAAAATCAAACATTGTTTTAGGAAACTTCCTGTCAATCGG5360.11618958845907894No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG5330.1155392735983005No Hit
TACTAACATGGGCCTAAAGATCAGGCAACTATTGTGGTTTCATATTTCTT5300.11488895873752208No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC5290.11467218711726261No Hit
GTATTAACATAATTGACTACTAGATCCCTGGATGCTGGATCTTCCAAATT5280.11445541549700312No Hit
CTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTTTTGCCTTCT5250.1138051006362247No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG5210.1129380141551868No Hit
CTATAGGCTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATACTC5200.11272124253492732No Hit
CTACTAGATACAGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGA5180.11228769929440838No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG5150.11163738443362996No Hit
CTTTAGGCCCATGTTAGTATTAACATAATTGACTACTAGATCCCTGGATG5150.11163738443362996No Hit
CTTTCGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC5120.11098706957285151No Hit
GAATACAGGTGCAATTTCCATCCGTAGGTTTTGTACAGCAACATGAGGGA5010.10860258174999729No Hit
ACTCAACCCTGTTCCAAATATTGCCTCTCACATCTCGTCAATCTCCTCGA5000.1083858101297378No Hit
AATCCACACTCCGAAAGAGACCAAATACTCAAGGACAGTTTCTCTTCCAA4980.10795226688921887No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG4970.10773549526895938No Hit
GATCAGGATAATAAGGTTTAATCCCTTTGTCTAGAGGCAAATATTTAGTG4960.10751872364869991No Hit
CTGTAAATGAAAAGAGAAGATTGAAATTAATTATGCCTGCTAGATTTTAT4900.10621809392714306No Hit
CTACATGATCAGGATAATAAGGTTTAATCCCTTTGTCTAGAGGCAAATAT4810.10426714934480777No Hit
GTACATGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAG4780.10361683448402935No Hit
CCTCTAGACAAAGGGATTAAACCTTATTATCCTGATCATGTAGTTAATCA4780.10361683448402935No Hit
GGGTTGAGTATTCCTGAACTGGAGCCACCAGCAGGAAAATACAGGCCCCT4740.10274974800299146No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC4740.10274974800299146No Hit
GTATGGTGAGGTGAGCAATGCTCAGGAGACTCTAAGGCTTCCCGATACAG4670.10123234666117513No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGCCT4650.10079880342065617No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)