FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002487924

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487924
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences645948
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN48280.7474285855827404No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA41930.6491234588542731No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT19550.30265594134512375No Hit
CACTAATATGGGCCTAAAGTTTAGGCAACTATTGTGGTTTCACATTTCTT18260.28268529355304145No Hit
GTGCTGGTGCGCACACCAATTTATGCCTACAGCCTCCTAATACAAACAGC16540.2560577631635983No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT14610.22617919708707201No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA14220.22014155938248897No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT13610.21069807476762833No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT12820.19846798813526784No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12500.1935140289930459No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT12340.1910370494219349No Hit
GGATAAGGGCATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT11960.18515422294054631No Hit
ATATTAGTGTTAACATAACTAACTACTAGGTCCCTGGATATTGGATCTTC11860.18360611070860192No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA11760.18205799847665757No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT11380.17617517199526897No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG11060.171221212853047No Hit
ATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTCACGGTGGTCTCCATGC11010.1704471567370748No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTGAAACATTCTTTGATTTTCTGT10700.16564800881804728No Hit
CACTCATATGGGCCTAAAGTTTAGGCAACTATTGTGGTTTCACATTTCTT10210.15806225888151987No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTC9960.15419197830165896No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA9950.15403716707846454No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT9780.1514053762841591No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAG9720.15047650894499248No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG9350.14474849368679832No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG9350.14474849368679832No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA9340.14459368246360388No Hit
CTCTACGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA9240.1430455702316595No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9240.1430455702316595No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT9200.14242632533888175No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT8940.13840123353582642No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC8780.13592425396471544No Hit
GGATAATAAGGTTTAATGCCCTTATCCAATGGCAAATATTTGGTAACATT8770.135769442741521No Hit
TTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC8660.1340665192863822No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGCATTAAACCTTAT8540.13220878460804894No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC8140.12601633568027146No Hit
CTCACAGTCAATGAAAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT8100.12539709078749373No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT7770.12028832042207732No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7610.11781134085096634No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG7530.11657285106541083No Hit
GATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTGG7520.11641803984221641No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT7220.1117737031463833No Hit
CATATAAGGGCTTTAAAGCAGGATAACCACATTGCGTAAAAGGGGCAGCA7110.11007077969124449No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA6990.10821304501291125No Hit
GCGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT6900.10681974400416133No Hit
GCTTTATTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCT6810.1054264429954114No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC6710.10387833076346703No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA6620.1024850297547171No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG6600.10217540730832822No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT6500.10062729507638386No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT6490.10047248385318941No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6460.10000805018360612No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)