FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002487926

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487926
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences507362
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA41300.8140144512202333No Hit
CATTTACACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGTCCACT23530.4637714294724477No Hit
CTCTATGGAAGGCGGGTATATTATATAAAAGAGAAACAACACGTAGCGCC20570.40543044216949636No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC16510.3254086825580158No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGACCACCGTG15750.31042923987212284No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT14220.28027325657025953No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT14090.2777109834792515No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG13820.2723893393671579No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT13060.25740989668126507No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAAC12720.25070856705862876No Hit
GTACCTGTCTTTAACCCTCATTGGAAAACACCTTCTTTTCCTAATATACA12060.23770010367351121No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT11400.2246916402883937No Hit
GGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATT11370.22410034649816107No Hit
ATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTCACGGTGGTCTCCATGC11270.22212936719738569No Hit
GTCCCACGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC11150.21976419203645525No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTC11130.21936999617630015No Hit
CACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGTCCACTCACAGT10510.2071499245114928No Hit
GTCTTTAACCCTCATTGGAAAACACCTTCTTTTCCTAATATACATTTACA9970.19650663628730572No Hit
CCTCCAGCTTATAGACCACCTAATGCCCCTATCTTATCAACACTTCCGGA9800.19315597147598756No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA9790.19295887354591001No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9570.18862271908420417No Hit
GCGTGGGACCATGCAGAACCTGCACGACTACTGTTCAAGGAACCTCTATG9330.18389236876234324No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT9030.1779794308600171No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATT8710.1716722970975359No Hit
AATATACATTTACACCAAGACATCATCAAAAAATGTGAACAATTTGTAGG8620.16989841572683803No Hit
TTCTTCCACTGTACCTGTCTTTAACCCTCATTGGAAAACACCTTCTTTTC8610.16970131779676048No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA8590.1693071219366054No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT8560.1687158281463728No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC8510.16773033849598512No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA8320.16398547782451187No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG8270.1629999881741242No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT8270.1629999881741242No Hit
CATTTGGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTCTCA8260.16280289024404668No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGT8080.15925512750265097No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT7970.15708705027179806No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG7860.15491897304094512No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA7850.15472187511086757No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7490.14762634962807622No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7450.14683795790776605No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCCACTGTACCTGTC7320.14427568481675807No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCCACTG7290.14368439102652544No Hit
GCTTTATTCTTCCACTGTACCTGTCTTTAACCCTCATTGGAAAACACCTT7230.1425018034460602No Hit
ATTTGTAGGTCCACTCACAGTTAATGAGAAACGAAGACTGCAATTAATTA7230.1425018034460602No Hit
ATATAAAAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT7200.14191050965582758No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC7180.1415163137956725No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT7160.14112211793551743No Hit
TCGCAGAAGATCTCAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACT6930.13658886554373406No Hit
GTGTAAATGTATATTAGGAAAAGAAGGTGTTTTCCAATGAGGGTTAAAGA6930.13658886554373406No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA6910.13619466968357896No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6890.13580047382342392No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGCTGGATCTTC6830.13461788624295867No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG6820.13442078831288115No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGACGAAACGG6720.13244980901210573No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTA6700.13205561315195066No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6700.13205561315195066No Hit
GGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTCTCATTAAC6670.13146431936171807No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC6670.13146431936171807No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC6600.1300846338511753No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTC6510.12831075248047744No Hit
GAATACAGGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA6500.1281136545503999No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCATCATACTGCACTCAGGCA6420.1265368711097796No Hit
ATCCAAATGTTACCAAATATTTGCCATTAGATAAGGGTATTAAACCTTAT6410.12633977317970208No Hit
GTTAATGAGAAACGAAGACTGCAATTAATTATGCCTGCTAGGTTTTATCC6350.12515718559923683No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC6310.12436879387892669No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT6260.12338330422853899No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC6250.12318620629846146No Hit
GGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAG6240.12298910836838392No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6220.12259491250822883No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA6050.1192442476969107No Hit
CCTAATGCCCCTATCTTATCAACACTTCCGGAGACTACTGTTGTTAGACG6030.11885005183675561No Hit
ACATAGAGGTTCCTTGAACAGTAGTCGTGCAGGTTCTGCATGGTCCCACG6000.118258758046523No Hit
CCACTGTACCTGTCTTTAACCCTCATTGGAAAACACCTTCTTTTCCTAAT5990.11806166011644546No Hit
CTTTAACCCTCATTGGAAAACACCTTCTTTTCCTAATATACATTTACACC5990.11806166011644546No Hit
GGCATAATTAATTGCAGTCTTCGTTTCTCATTAACTGTGAGTGGACCTAC5960.11747036632621284No Hit
CTTTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT5960.11747036632621284No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAAAAAATCAAAGAATGT5950.11727326839613529No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG5890.11609068081567007No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAAAAA5870.11569648495551499No Hit
ATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAA5870.11569648495551499No Hit
GGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAA5870.11569648495551499No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC5860.11549938702543747No Hit
GGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACA5850.11530228909535992No Hit
GTCTATAAGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATAC5770.11372550565473961No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG5740.11313421186450699No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5680.11195162428404178No Hit
AGATCACATCATACAAAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTA5620.11076903670357655No Hit
GTCGTTGACATTGCAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG5590.11017774291334392No Hit
GTATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCATCATACTG5580.10998064498326637No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC5560.1095864491231113No Hit
TCTAGGGACCTAGTAGTCAGTTATGTCAACACTAATATGGGCCTAAAGTT5560.1095864491231113No Hit
ATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCCATGAA5560.1095864491231113No Hit
CAGTTAATGAGAAACGAAGACTGCAATTAATTATGCCTGCTAGGTTTTAT5560.1095864491231113No Hit
CCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAAGAGTCCTCTTATGT5550.10938935119303377No Hit
GACAGGTACAGTGGAAGAATAAAGCCCCGTAAAGTTTCCCACCTTATGAG5480.108009665682491No Hit
CTTTAAATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAA5330.10505319673132793No Hit
GTTATGTCAACACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTT5270.1038706091508627No Hit
GACATAGCCCATGAAATTTAAAGAGTAACCCCATCTTTTAGTTTTGTTAG5250.10347641329070763No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG5250.10347641329070763No Hit
CTCCGAAAGACACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGA5230.10308221743055253No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG5200.10249092364031993No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5100.10051994433954453No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC5080.10012574847938947No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA5080.10012574847938947No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)