FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002487927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences507362
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA41730.8224896622135673No Hit
CATTTACACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGTCCACT20280.3997146021972477No Hit
CTCTATGGAAGGCGGGTATATTATATAAAAGAGAAACAACACGTAGCGCC19110.37665414437817574No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGACCACCGTG16260.32048123430607733No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG16090.31713056949475915No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAAC15950.3143711984736736No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT14320.28224423587103487No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC13900.27396612280777827No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT13680.26962996834607245No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT12720.25070856705862876No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT12230.24105076848482937No Hit
GGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATT11940.23533492851258078No Hit
GTACCTGTCTTTAACCCTCATTGGAAAACACCTTCTTTTCCTAATATACA11670.23001328440048724No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA10570.208332512091958No Hit
GTCCCACGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC9980.19670373421738324No Hit
CCTCCAGCTTATAGACCACCTAATGCCCCTATCTTATCAACACTTCCGGA9900.19512695077676295No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATT9450.18625754392327373No Hit
GTCTTTAACCCTCATTGGAAAACACCTTCTTTTCCTAATATACATTTACA9190.18113299774125774No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA9060.17857072465024973No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9040.17817652879009463No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG8810.17364327639831126No Hit
CATTTGGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTCTCA8650.17048970951707065No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT8510.16773033849598512No Hit
GCGTGGGACCATGCAGAACCTGCACGACTACTGTTCAAGGAACCTCTATG8400.16556226126513218No Hit
CACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGTCCACTCACAGT8370.16497096747489956No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA8220.1620144985237365No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7960.1568899523417205No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT7810.15393348339055743No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGT7780.15334218960032484No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT7730.15235669994993714No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTA7620.1501886227190842No Hit
GGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAA7420.14624666411753343No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT7380.14545827239722328No Hit
AATATACATTTACACCAAGACATCATCAAAAAATGTGAACAATTTGTAGG7260.14309309723629282No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7260.14309309723629282No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA7220.14230470551598268No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC7160.14112211793551743No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT7100.13993953035505222No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTC7080.13954533449489714No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG7030.13855984484450945No Hit
GTGTAAATGTATATTAGGAAAAGAAGGTGTTTTCCAATGAGGGTTAAAGA7020.1383627469144319No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG6940.13678596347381158No Hit
TTCTTCCACTGTACCTGTCTTTAACCCTCATTGGAAAACACCTTCTTTTC6910.13619466968357896No Hit
CTTTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT6860.1352091800331913No Hit
ATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTCACGGTGGTCTCCATGC6850.13501208210311377No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG6820.13442078831288115No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCCACTG6740.13284400487226083No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC6700.13205561315195066No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGCTGGATCTTC6670.13146431936171807No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC6660.13126722143164052No Hit
ATTTGTAGGTCCACTCACAGTTAATGAGAAACGAAGACTGCAATTAATTA6620.13047882971133037No Hit
GGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACA6490.12791655662032236No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6390.125945577319547No Hit
GTCTATAAGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATAC6370.12555138145939193No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC6270.12358040215861651No Hit
TCGCAGAAGATCTCAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACT6250.12318620629846146No Hit
GGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTCTCATTAAC6240.12298910836838392No Hit
CTTTAACCCTCATTGGAAAACACCTTCTTTTCCTAATATACATTTACACC6240.12298910836838392No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC6150.12121522699768607No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAAAAAATCAAAGAATGT6140.12101812906760853No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCCACTGTACCTGTC6060.11944134562698822No Hit
GTTAATGAGAAACGAAGACTGCAATTAATTATGCCTGCTAGGTTTTATCC6020.11865295390667807No Hit
ATCCAAATGTTACCAAATATTTGCCATTAGATAAGGGTATTAAACCTTAT6010.11845585597660054No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCATCATACTGCACTCAGGCA6010.11845585597660054No Hit
ATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAA5990.11806166011644546No Hit
GAATACAGGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA5970.11766746425629039No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG5920.1166819746059027No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5870.11569648495551499No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAAAAA5860.11549938702543747No Hit
GCTTTATTCTTCCACTGTACCTGTCTTTAACCCTCATTGGAAAACACCTT5790.11411970151489469No Hit
ATATAAAAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT5790.11411970151489469No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG5760.11352840772466208No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC5730.11293711393442947No Hit
GGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAG5670.11175452635396423No Hit
ATTAATTGCAGTCTTCGTTTCTCATTAACTGTGAGTGGACCTACAAATTG5660.11155742842388669No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5640.11116323256373162No Hit
GGCATAATTAATTGCAGTCTTCGTTTCTCATTAACTGTGAGTGGACCTAC5630.11096613463365407No Hit
CTCCGAAAGACACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGA5620.11076903670357655No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC5570.10978354705318885No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTC5570.10978354705318885No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA5560.1095864491231113No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA5500.10840386154264607No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC5460.10761546982233593No Hit
CTTTAAATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAA5360.10564449052156055No Hit
CCACTGTACCTGTCTTTAACCCTCATTGGAAAACACCTTCTTTTCCTAAT5350.105447392591483No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5350.105447392591483No Hit
ATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCCATGAA5250.10347641329070763No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT5230.10308221743055253No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT5160.10170253192000978No Hit
GTATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCATCATACTG5150.10150543398993223No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG5090.10032284640946701No Hit
AGATCACATCATACAAAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTA5080.10012574847938947No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)