FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002487929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences566497
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA41870.7391036492691047No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19170.33839543722208587No Hit
CATTTACACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGTCCACT18040.3184482883404502No Hit
CTTTAGGACATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGGA16440.2902045377115854No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC13550.23918926313819844No Hit
GGAAAACACCTTCTTTTCCTAATATACATTTACACCAAGACATCATCAAA13090.2310691848323998No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT12110.21376988757222018No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT11910.21023941874361204No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC11500.20300195764496545No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT11400.2012367232306614No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT11210.19788277784348374No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC11040.19488187933916684No Hit
CCTCCAGCTTATAGACCACCTAATGCCCCTATCTTATCAACACTTCCGGA10930.1929401214834324No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10800.19064531674483715No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGACCACCGTG10350.1827017618804689No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT10230.18058348058330406No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA10200.18005391025901285No Hit
GTGTAAAAGGGGCAGCGAAACCCAAAAGACCCACAATACGTTGACAGACT10100.17828867584470878No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCTTCTTTTCCTAAT10030.17705301175469595No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG9540.16840336312460613No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA9350.16504941773742846No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT9150.16151894890882035No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATT8940.15781195663878184No Hit
CAATAGGCCTATTAACAGGAAGTTTTCTAAAACATTCTCTGATTTTCTGT8370.14775012047724878No Hit
GTCTTTAACCCTCATTGGAAAACACCTTCTTTTCCTAATATACATTTACA8310.14669097982866636No Hit
CCCTCACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCATGGGCTATG8070.14245441723433663No Hit
CACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGTCCACTCACAGT7880.13910047184715896No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCTTCTTTTC7260.12815601847847385No Hit
CCCTTATCTAATGGCAAATATTTGGTACCATTTGGGTAAAACCTAGCAGG7210.12727340127132183No Hit
GTCCCACGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC7210.12727340127132183No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTA7020.12391945588414413No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTACCATTTGGGTAAAAC6950.1226837917941313No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCTT6740.1189767995240928No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC6660.11756461199264956No Hit
GGTACCATTTGGGTAAAACCTAGCAGGCATAATTAATTGCAGTCTTCGTT6550.11562285413691512No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG6460.11403414316404147No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT6440.11368109628118067No Hit
TAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT6420.11332804939831985No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6330.11173933842544621No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA6310.1113862915425854No Hit
CATATAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT6300.11120976810115499No Hit
AATATACATTTACACCAAGACATCATCAAAAAATGTGAACAATTTGTAGG6290.11103324465972458No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT6280.11085672121829418No Hit
ATTTGTAGGTCCACTCACAGTCAATGAGAAACGAAGACTGCAATTAATTA6260.11050367433543339No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA6230.10997410401114216No Hit
CTCATTGACTGTGAGTGGACCTACAAATTGTTCACATTTTTTGATGATGT6160.10873843992112932No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTACCTGTC6160.10873843992112932No Hit
GCTTTAGGACATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGG6150.10856191647969891No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCATCTACCAATCGCCAGTC5990.10573754141681244No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT5960.10520797109252124No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5890.1039723070025084No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA5860.10344273667821718No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5830.10291316635392597No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)