FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences570045
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN87271.5309317685445885No Hit
TCCATAGTGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA28190.49452236226964535No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT24390.4278609583453938No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGGTAAAACCTAGCAGG15370.26962783639888077No Hit
CTTATATAATATACCCGCCTTCCATAGTGTGTGTAAATAATGTCTGGTTT15150.2657684919611609No Hit
CTCTTATATAATATACCCGCCTTCCATAGTGTGTGTAAATAATGTCTGGT14770.2591023515687358No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGGTAAAAC14540.2550675823838469No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT14240.2498048399687744No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT13730.24085817786315117No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT13570.23805138190844582No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT13240.23226236525186608No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT13150.23068354252734433No Hit
TTACAGGGCTTTATTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAA13080.22945556929716074No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC11770.20647492741801085No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT11620.20384355621047462No Hit
ATATAATATACCCGCCTTCCATAGTGTGTGTAAATAATGTCTGGTTTGGA11560.20279100772746012No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT11280.1978791148067258No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC11270.1977036900595567No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT11180.19612486733503495No Hit
CTCACAGTCAATGAAAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTT10760.18875702795393348No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10320.18103833907849382No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTAC10120.17752984413511214No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA10050.17630187090492855No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG9570.16788148304081257No Hit
CTTCCATAGTGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTA9180.16103991790121833No Hit
TAATATACCCGCCTTCCATAGTGTGTGTAAATAATGTCTGGTTTGGAAGT8830.15490005175030042No Hit
ACCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT8180.14349744318431001No Hit
ATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTACTGAAGGAAAGAAGT8010.1405152224824356No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG7940.13928724925225203No Hit
GGCATAATCAATTGCAATCTTCTTTTTTCATTGACTGTGAGTGGGCCTAC7930.13911182450508291No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA7890.1384101255164066No Hit
TTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC7820.13718215228622302No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC7550.13244568411265778No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC7480.1312177108824742No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA7120.1249024199843872No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG7110.12472699523721811No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC7080.12420072099571086No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT7050.12367444675420362No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA7010.12297274776552729No Hit
TCCTAATACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGT6980.12244647352402004No Hit
GATTATGCCTGCTAGGTTTTACCCAAATGTTACCAAATATTTGCCATTGG6960.12209562402968187No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC6880.1206922260523292No Hit
ATCCAATATCCAGGGACCTAGTAGTCAGTTATGTTAACACTAATATGGGC6720.11788543009762387No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6720.11788543009762387No Hit
GTTATGTTAACACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTT6630.11630660737310212No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6520.11437693515424222No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA6450.11314896192405861No Hit
ATAATATACCCGCCTTCCATAGTGTGTGTAAATAATGTCTGGTTTGGAAG6450.11314896192405861No Hit
CACTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC6430.11279811242972046No Hit
CTCTAAGGCTTCCCGATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTA6300.11051759071652237No Hit
GCTTTATTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCT6210.10893876799200064No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC6200.10876334324483154No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGGTAAAA6110.10718452052030981No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT6100.1070090957731407No Hit
TAACCACATTGTGTGAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG6080.10665824627880255No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGATGTGAT6020.10560569779578806No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG6020.10560569779578806No Hit
TTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAAC6010.10543027304861897No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA5940.10420229981843539No Hit
GGCAAATATTTGGTAACATTTGGGTAAAACCTAGCAGGCATAATCAATTG5940.10420229981843539No Hit
CCTTCCATAGTGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACT5920.10385145032409723No Hit
CTAATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTA5890.10332517608258997No Hit
CTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC5870.10297432658825181No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT5870.10297432658825181No Hit
ACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCA5770.10122007911656097No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)