FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002487931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences570045
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGTGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA27980.4908384425790946No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT24170.424001613907674No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21790.3822505240814322No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGGTAAAACCTAGCAGG17000.29822207018744135No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGGTAAAAC16610.2913805050478471No Hit
CTCTTATATAATATACCCGCCTTCCATAGTGTGTGTAAATAATGTCTGGT15190.2664701909498373No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT14650.2569972546027068No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT14170.2485768667385908No Hit
CTTATATAATATACCCGCCTTCCATAGTGTGTGTAAATAATGTCTGGTTT13990.2454192212895473No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT13220.23191151575752794No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT13020.22840302081414626No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT12740.22349112789341194No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC12570.2205089071915375No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC12470.21875465971984667No Hit
ATATAATATACCCGCCTTCCATAGTGTGTGTAAATAATGTCTGGTTTGGA12150.213141067810436No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT11820.2073520511538563No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA11680.2048961046934891No Hit
TTACAGGGCTTTATTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAA11620.20384355621047462No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT11570.2029664324746292No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT11500.2017384592444456No Hit
CTCACAGTCAATGAAAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTT11010.19314264663316055No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTAC10730.1882307537124262No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG9900.17367049969739232No Hit
CTTCCATAGTGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTA9800.17191625222570148No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9470.16612723556912173No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG9010.1580576971993439No Hit
TAATATACCCGCCTTCCATAGTGTGTGTAAATAATGTCTGGTTTGGAAGT8980.15753142295783668No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA8770.1538475032672859No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA8590.15068985781824243No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG8550.1499881588295661No Hit
TTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC8050.14121692147111192No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC7920.13893639975791383No Hit
ACCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT7800.13683130279188485No Hit
GGCATAATCAATTGCAATCTTCTTTTTTCATTGACTGTGAGTGGGCCTAC7730.13560332956170126No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT7340.12876176442210702No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGGTAAAA7330.12858633967493793No Hit
GATTATGCCTGCTAGGTTTTACCCAAATGTTACCAAATATTTGCCATTGG7220.12665666745607804No Hit
ATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTACTGAAGGAAAGAAGT7170.12577954372023262No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7160.12560411897306353No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC6920.12139392504100553No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT6810.11946425282214562No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6760.11858712908630022No Hit
CTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC6670.11700830636177846No Hit
CCTTCCATAGTGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACT6580.1154294836372567No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA6520.11437693515424222No Hit
GGCAAATATTTGGTAACATTTGGGTAAAACCTAGCAGGCATAATCAATTG6460.11332438667122771No Hit
GTTATGTTAACACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTT6440.11297353717688954No Hit
ATCCAATATCCAGGGACCTAGTAGTCAGTTATGTTAACACTAATATGGGC6430.11279811242972046No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA6390.11209641344104412No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA6340.1112192897051987No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC6270.10999131647501513No Hit
CACTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC6230.1092896174863388No Hit
CATTTGGGTAAAACCTAGCAGGCATAATCAATTGCAATCTTCTTTTTTCA6210.10893876799200064No Hit
ATAATATACCCGCCTTCCATAGTGTGTGTAAATAATGTCTGGTTTGGAAG6070.10648282153163348No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGATGTGAT6060.1063073967844644No Hit
CTCCGAAAGACACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGA6040.10595654729012621No Hit
GCTTTATTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCT6030.10578112254295713No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG6000.10525484830144988No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC5990.1050794235542808No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC5990.1050794235542808No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG5980.10490399880711172No Hit
GTGTTGTTTCTCTCTTATATAATATACCCGCCTTCCATAGTGTGTGTAAA5980.10490399880711172No Hit
TCCTAATACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGT5970.10472857405994264No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG5950.10437772456560446No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG5840.10244805234674455No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC5740.10069380487505372No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)