FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487932

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487932
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437033
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA30680.7020064846361717No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG23500.5377168314520872No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT22980.5258184164582538No Hit
CAATTCGTTGGCACACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTT19920.4558008205330032No Hit
CATACAAGGGCATTAAAGCAGGATAACCACATTGAGTAAAAGGGGCAGCA19680.4503092443820032No Hit
GTACCGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC16510.377774676054211No Hit
CCTCCAGCTTATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA15140.3464269288589191No Hit
ATCTAAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG14390.329265753387044No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT13080.2992909002295021No Hit
CATATAAGGGCATTAAAGCAGGATAACCACATTGAGTAAAAGGGGCAGCA12460.28510432850608536No Hit
CCTTATATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACT11790.2697736784178769No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA11740.2686296000530852No Hit
CTCTATGGCAGGCGGGTATCTTATATAAGAGAGAAACAACACATAGCGCC11390.2606210514995435No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC11230.25696000073221015No Hit
CCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG11120.2544430283296685No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC10410.2381971155496267No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTACTCTCTGAATTTCAT9970.22812922593945995No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAAC9620.22012067738591823No Hit
CATTTAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT9480.2169172579645015No Hit
GTGTGTAGCATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTG9350.2139426542160432No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9310.21302739152420985No Hit
GAAAAGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTAGATTGAATA9120.20867989373800147No Hit
ATCTTAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG9100.2082222623920848No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTT8450.19334924364979306No Hit
GCTACACACTGTGCCAGCAAATCCGCCTCCTGCCTCCACCAATCGCCAGT8410.1924339809579597No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGGACAACAGAGT8160.18671358913400132No Hit
CTATTTACACACTCTATGGCAGGCGGGTATCTTATATAAGAGAGAAACAA7940.18167964432891795No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTC7910.18099319731004296No Hit
GCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGT7910.18099319731004296No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAAC7900.1807643816370846No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGT7840.1793914875993346No Hit
GTATATGGTGACCCACAAAATGAGGCGCTATGTGTTGTTTCTCTCTTATA7810.1787050405804596No Hit
CTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAACATACATTTACACC7750.1773321465427096No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCT7630.17458635846720957No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTCTTGTGGTTTCACATTTCTT7490.17138293904579288No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC7400.16932359798916785No Hit
CTGTCTGGCTTTTAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTG7340.16795070395141787No Hit
AGTATATGGTGACCCACAAAATGAGGCGCTATGTGTTGTTTCTCTCTTAT7290.16680662558662618No Hit
AGTCTGTACCGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT7210.1649761002029595No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAGTAAT7070.1617726807815428No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG6980.1597133397249178No Hit
CTCTTATATAAGATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGT6950.15902689270604278No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA6870.1571963673223761No Hit
AACGCATTGTGGGTCTTTTGGGTTTTGCTGCCCCTTTTACTCAATGTGGT6860.15696755164941778No Hit
CCATATACTTGGGAACAAGAGCTACAGCATGGGGCAGAATCTTTCCACCA6850.15673873597645943No Hit
CTGTATCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAAGAGTTGC6840.1565099203035011No Hit
CTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAAGAGTTGC6760.15467939491983443No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC6680.15284886953616775No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC6660.15239123819025108No Hit
CCCAAAGTGAGAGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGAAC6640.1519336068443344No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTACCTGTC6550.1498742657877094No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT6530.14941663444179273No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATATTCTATTA6400.14644203069333436No Hit
CCCTAACAAAACAAAGAGATGGGGTTACTCTCTGAATTTCATGGGCTATG6290.1439250582907927No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGCTGGATCTTC6220.14232334858008433No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCTTTTCTGTGTAAACAATACC6190.14163690156120934No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG6070.13889111348570932No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG6050.13843348213979265No Hit
GTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGG5990.13706058810204264No Hit
GGATAACCACATTGAGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT5970.13660295675612596No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC5940.13591650973725097No Hit
GTCGTTGACATTGCAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG5940.13591650973725097No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAAGCCTTGTAAGTTGGCGA5910.13523006271837595No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5870.1343148000265426No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5860.13408598435358426No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAGACTACTGTTGTTAG5720.1308825649321676No Hit
ATTTTGTGGTCTATAAGCTGGAGGAGTGCGAATCCACACTCCGAAAGACA5700.13042493358625093No Hit
ATGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG5520.1263062514730009No Hit
CTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTG5450.12470454176229254No Hit
GAACCACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC5410.12378927907045921No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT5380.1231028320515842No Hit
GAGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCGTTGGCACACT5370.12287401637862587No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG5370.12287401637862587No Hit
GGGTTACTCTCTGAATTTCATGGGCTATGTCATTGGATGTTATGGGTCAT5310.12150112234087584No Hit
GTATGGATCGGCAGAGGAGCCGTAAAGGTTCCACGCATGCGCTGATGGCC5310.12150112234087584No Hit
GTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAACATACATTTACA5300.12127230666791752No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC5280.12081467532200085No Hit
GTGTTGTTTCTCTCTTATATAAGATACCCGCCTGCCATAGAGTGTGTAAA5230.11967059695720918No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA5220.11944178128425086No Hit
GCGTTCTATCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGT5130.11738244022762583No Hit
CTTATATAAGATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTT5080.11623836186283416No Hit
AGGCCTTTCTGTGTAAACAATACCTGAACCTTTACCCCGTTGTCCGGCAA5070.11600954618987583No Hit
CACTCTGGGATCTTGCAGAGTTTGGTGGAAGGTTGTGGAGTTCCACTGCA5050.11555191484395916No Hit
GAGATACAGTAATAGAATATTTGGTGTCTTTCGGAGTGTGGATTCGCACT5050.11555191484395916No Hit
TCCGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG5020.11486546782508414No Hit
GGGTCCTAGGAATCCTGATGTGATGTTCTCCATATTCAGCGCAGGGTCCC5010.1146366521521258No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGACTTAATGACTCTA4970.11372138946029248No Hit
GTGTAAATGTATGTTAGGAAAAGAGGGTGTTTTCCAATGAGGATTAAAGA4970.11372138946029248No Hit
GAGAAACAGTAATAGAATATTTGGTGTCTTTCGGAGTGTGGATTCGCACT4930.11280612676845914No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG4910.11234849542254247No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCGAGGGGGAACCACCGTG4900.11211967974958413No Hit
CCTTTACCCCGTTGTCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4900.11211967974958413No Hit
ACCATATACTTGGGAACAAGAGCTACAGCATGGGGCAGAATCTTTCCACC4780.10937389167408411No Hit
CCTTTGGATAAAACCTAGCAGGCATAATCAATTGCAATCTTCTTTTCTCA4770.10914507600112577No Hit
CCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAAGAGTCCTCTTATGT4760.10891626032816745No Hit
CAATGCGTTGGCACACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTT4760.10891626032816745No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT4750.10868744465520912No Hit
GTATCGGGATGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTG4700.10754336629041743No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT4690.10731455061745909No Hit
CTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCT4670.10685691927154242No Hit
ACTAAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACAAATGG4640.10617047225266743No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4630.1059416565797091No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGTCCGGCAACGGCCAGGTC4600.10525520956083406No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA4600.10525520956083406No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4590.10502639388787574No Hit
GTCCCGTGCTGGTGGTTGAGGATCCTGGAAGTAGAGGACAAACGGGCAAC4570.10456876254195907No Hit
GGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGAACACATCATACA4550.1041111311960424No Hit
CCTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACT4540.10388231552308407No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAA4500.10296705283125074No Hit
GCCGTAAAGGTTCCACGCATGCGCTGATGGCCCATGACCAAGCCCCAGCC4500.10296705283125074No Hit
TCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGACTTAATGA4490.1027382371582924No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG4470.10228060581237573No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACT4460.10205179013941738No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)